Ralstonia phage RSL2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Chiangmaivirus; Ralstonia virus RSL2

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8JBF8|A0A0A8JBF8_9CAUD Uncharacterized protein OS=Ralstonia phage RSL2 OX=1585840 PE=4 SV=1
MM1 pKa = 7.47LVFLLVEE8 pKa = 4.31KK9 pKa = 10.85ADD11 pKa = 3.87DD12 pKa = 3.9AFDD15 pKa = 3.8YY16 pKa = 11.44VFGQCCEE23 pKa = 4.39GFDD26 pKa = 3.86SQHH29 pKa = 6.68LGFEE33 pKa = 4.41FVEE36 pKa = 4.31NFVDD40 pKa = 4.21DD41 pKa = 3.96AHH43 pKa = 6.96DD44 pKa = 3.92FSLKK48 pKa = 10.64GVFF51 pKa = 4.14

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8J9C6|A0A0A8J9C6_9CAUD Uncharacterized protein OS=Ralstonia phage RSL2 OX=1585840 PE=4 SV=2
MM1 pKa = 7.36QSAPQTNMAILANGAEE17 pKa = 4.43LTRR20 pKa = 11.84NTVASMMTNEE30 pKa = 3.8NVRR33 pKa = 11.84NFFGQPAARR42 pKa = 11.84DD43 pKa = 3.29TVYY46 pKa = 10.49RR47 pKa = 11.84VFMMKK52 pKa = 8.65VHH54 pKa = 6.65KK55 pKa = 10.15KK56 pKa = 10.88LEE58 pKa = 4.22GALRR62 pKa = 11.84NMPSGQFNKK71 pKa = 9.99SVRR74 pKa = 11.84TGEE77 pKa = 4.24HH78 pKa = 4.72QSGAVNPNVIAKK90 pKa = 9.77

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

68019

31

2039

287.0

32.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.857 ± 0.303

0.844 ± 0.052

6.717 ± 0.136

6.263 ± 0.204

4.408 ± 0.105

6.511 ± 0.229

2.077 ± 0.087

5.791 ± 0.126

6.073 ± 0.227

8.149 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.139 ± 0.093

5.046 ± 0.124

4.336 ± 0.107

4.283 ± 0.129

4.938 ± 0.186

5.856 ± 0.143

5.957 ± 0.192

6.5 ± 0.124

1.285 ± 0.06

3.971 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski