Amino acid dipepetide frequency for Ralstonia phage RSL2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.277AlaAla: 8.277 ± 1.112
0.794AlaCys: 0.794 ± 0.136
4.984AlaAsp: 4.984 ± 0.375
4.293AlaGlu: 4.293 ± 0.359
2.896AlaPhe: 2.896 ± 0.204
5.528AlaGly: 5.528 ± 0.478
1.235AlaHis: 1.235 ± 0.129
4.543AlaIle: 4.543 ± 0.26
4.219AlaLys: 4.219 ± 0.293
6.542AlaLeu: 6.542 ± 0.32
2.926AlaMet: 2.926 ± 0.189
3.617AlaAsn: 3.617 ± 0.244
2.882AlaPro: 2.882 ± 0.39
3.587AlaGln: 3.587 ± 0.335
3.764AlaArg: 3.764 ± 0.246
4.514AlaSer: 4.514 ± 0.266
4.661AlaThr: 4.661 ± 0.471
5.16AlaVal: 5.16 ± 0.351
1.044AlaTrp: 1.044 ± 0.131
3.102AlaTyr: 3.102 ± 0.235
0.0AlaXaa: 0.0 ± 0.0
Cys
0.603CysAla: 0.603 ± 0.089
0.176CysCys: 0.176 ± 0.05
0.426CysAsp: 0.426 ± 0.074
0.588CysGlu: 0.588 ± 0.101
0.485CysPhe: 0.485 ± 0.103
0.456CysGly: 0.456 ± 0.077
0.338CysHis: 0.338 ± 0.082
0.397CysIle: 0.397 ± 0.074
0.559CysLys: 0.559 ± 0.102
0.617CysLeu: 0.617 ± 0.095
0.265CysMet: 0.265 ± 0.058
0.412CysAsn: 0.412 ± 0.078
0.353CysPro: 0.353 ± 0.084
0.353CysGln: 0.353 ± 0.079
0.412CysArg: 0.412 ± 0.082
0.603CysSer: 0.603 ± 0.097
0.441CysThr: 0.441 ± 0.069
0.5CysVal: 0.5 ± 0.089
0.162CysTrp: 0.162 ± 0.042
0.294CysTyr: 0.294 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
5.352AspAla: 5.352 ± 0.427
0.5AspCys: 0.5 ± 0.084
3.823AspAsp: 3.823 ± 0.329
4.469AspGlu: 4.469 ± 0.296
3.279AspPhe: 3.279 ± 0.207
4.219AspGly: 4.219 ± 0.252
1.411AspHis: 1.411 ± 0.143
4.087AspIle: 4.087 ± 0.235
3.72AspLys: 3.72 ± 0.276
6.278AspLeu: 6.278 ± 0.298
2.338AspMet: 2.338 ± 0.169
2.793AspAsn: 2.793 ± 0.212
3.528AspPro: 3.528 ± 0.185
2.676AspGln: 2.676 ± 0.216
3.631AspArg: 3.631 ± 0.498
3.47AspSer: 3.47 ± 0.242
3.661AspThr: 3.661 ± 0.246
4.175AspVal: 4.175 ± 0.284
1.117AspTrp: 1.117 ± 0.139
2.646AspTyr: 2.646 ± 0.214
0.0AspXaa: 0.0 ± 0.0
Glu
4.484GluAla: 4.484 ± 0.259
0.515GluCys: 0.515 ± 0.084
4.308GluAsp: 4.308 ± 0.25
4.631GluGlu: 4.631 ± 0.347
2.926GluPhe: 2.926 ± 0.25
3.646GluGly: 3.646 ± 0.239
1.588GluHis: 1.588 ± 0.191
4.161GluIle: 4.161 ± 0.285
4.219GluLys: 4.219 ± 0.307
5.719GluLeu: 5.719 ± 0.3
2.014GluMet: 2.014 ± 0.221
2.485GluAsn: 2.485 ± 0.182
2.102GluPro: 2.102 ± 0.216
2.735GluGln: 2.735 ± 0.255
3.146GluArg: 3.146 ± 0.279
3.308GluSer: 3.308 ± 0.215
2.999GluThr: 2.999 ± 0.213
4.234GluVal: 4.234 ± 0.315
0.853GluTrp: 0.853 ± 0.124
2.558GluTyr: 2.558 ± 0.27
0.0GluXaa: 0.0 ± 0.0
Phe
3.102PheAla: 3.102 ± 0.224
0.382PheCys: 0.382 ± 0.07
3.543PheAsp: 3.543 ± 0.222
2.985PheGlu: 2.985 ± 0.217
1.882PhePhe: 1.882 ± 0.169
3.087PheGly: 3.087 ± 0.206
0.794PheHis: 0.794 ± 0.116
2.249PheIle: 2.249 ± 0.201
2.632PheLys: 2.632 ± 0.21
3.014PheLeu: 3.014 ± 0.216
1.529PheMet: 1.529 ± 0.149
2.441PheAsn: 2.441 ± 0.223
1.764PhePro: 1.764 ± 0.16
1.603PheGln: 1.603 ± 0.155
2.073PheArg: 2.073 ± 0.183
2.632PheSer: 2.632 ± 0.206
2.896PheThr: 2.896 ± 0.281
3.132PheVal: 3.132 ± 0.237
0.456PheTrp: 0.456 ± 0.084
1.882PheTyr: 1.882 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
4.616GlyAla: 4.616 ± 0.45
0.382GlyCys: 0.382 ± 0.07
4.278GlyAsp: 4.278 ± 0.307
3.97GlyGlu: 3.97 ± 0.226
3.132GlyPhe: 3.132 ± 0.225
4.969GlyGly: 4.969 ± 0.537
1.514GlyHis: 1.514 ± 0.138
3.837GlyIle: 3.837 ± 0.243
4.219GlyLys: 4.219 ± 0.388
4.852GlyLeu: 4.852 ± 0.293
1.955GlyMet: 1.955 ± 0.162
3.22GlyAsn: 3.22 ± 0.289
2.132GlyPro: 2.132 ± 0.399
2.837GlyGln: 2.837 ± 0.258
3.043GlyArg: 3.043 ± 0.242
4.175GlySer: 4.175 ± 0.286
4.293GlyThr: 4.293 ± 0.42
4.264GlyVal: 4.264 ± 0.229
0.823GlyTrp: 0.823 ± 0.121
2.602GlyTyr: 2.602 ± 0.213
0.0GlyXaa: 0.0 ± 0.0
His
1.176HisAla: 1.176 ± 0.153
0.221HisCys: 0.221 ± 0.059
1.235HisAsp: 1.235 ± 0.128
1.5HisGlu: 1.5 ± 0.138
0.956HisPhe: 0.956 ± 0.107
1.044HisGly: 1.044 ± 0.098
0.632HisHis: 0.632 ± 0.108
1.397HisIle: 1.397 ± 0.154
1.117HisLys: 1.117 ± 0.151
1.882HisLeu: 1.882 ± 0.179
0.735HisMet: 0.735 ± 0.115
1.014HisAsn: 1.014 ± 0.12
1.294HisPro: 1.294 ± 0.136
0.706HisGln: 0.706 ± 0.095
1.264HisArg: 1.264 ± 0.135
0.912HisSer: 0.912 ± 0.122
0.97HisThr: 0.97 ± 0.12
1.5HisVal: 1.5 ± 0.165
0.279HisTrp: 0.279 ± 0.054
0.941HisTyr: 0.941 ± 0.107
0.0HisXaa: 0.0 ± 0.0
Ile
4.308IleAla: 4.308 ± 0.273
0.485IleCys: 0.485 ± 0.075
4.572IleAsp: 4.572 ± 0.24
3.999IleGlu: 3.999 ± 0.243
2.485IlePhe: 2.485 ± 0.209
3.573IleGly: 3.573 ± 0.221
1.235IleHis: 1.235 ± 0.125
3.249IleIle: 3.249 ± 0.252
3.279IleLys: 3.279 ± 0.258
4.087IleLeu: 4.087 ± 0.226
1.47IleMet: 1.47 ± 0.151
3.22IleAsn: 3.22 ± 0.259
2.999IlePro: 2.999 ± 0.224
2.529IleGln: 2.529 ± 0.206
2.985IleArg: 2.985 ± 0.246
3.69IleSer: 3.69 ± 0.242
2.852IleThr: 2.852 ± 0.207
3.911IleVal: 3.911 ± 0.231
0.573IleTrp: 0.573 ± 0.085
2.411IleTyr: 2.411 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
4.455LysAla: 4.455 ± 0.369
0.338LysCys: 0.338 ± 0.077
3.999LysAsp: 3.999 ± 0.314
3.925LysGlu: 3.925 ± 0.335
2.588LysPhe: 2.588 ± 0.194
3.455LysGly: 3.455 ± 0.341
1.294LysHis: 1.294 ± 0.142
3.867LysIle: 3.867 ± 0.207
4.808LysLys: 4.808 ± 0.347
5.234LysLeu: 5.234 ± 0.33
1.676LysMet: 1.676 ± 0.152
3.249LysAsn: 3.249 ± 0.206
2.617LysPro: 2.617 ± 0.22
2.749LysGln: 2.749 ± 0.241
2.837LysArg: 2.837 ± 0.232
3.146LysSer: 3.146 ± 0.244
3.94LysThr: 3.94 ± 0.265
3.528LysVal: 3.528 ± 0.244
0.617LysTrp: 0.617 ± 0.093
2.411LysTyr: 2.411 ± 0.195
0.0LysXaa: 0.0 ± 0.0
Leu
6.116LeuAla: 6.116 ± 0.306
0.75LeuCys: 0.75 ± 0.107
5.352LeuAsp: 5.352 ± 0.246
5.102LeuGlu: 5.102 ± 0.337
3.323LeuPhe: 3.323 ± 0.241
4.984LeuGly: 4.984 ± 0.265
1.5LeuHis: 1.5 ± 0.147
3.734LeuIle: 3.734 ± 0.234
5.484LeuLys: 5.484 ± 0.284
6.395LeuLeu: 6.395 ± 0.333
2.396LeuMet: 2.396 ± 0.214
4.44LeuAsn: 4.44 ± 0.267
3.984LeuPro: 3.984 ± 0.254
3.411LeuGln: 3.411 ± 0.219
4.293LeuArg: 4.293 ± 0.225
5.734LeuSer: 5.734 ± 0.292
5.337LeuThr: 5.337 ± 0.261
4.999LeuVal: 4.999 ± 0.271
1.103LeuTrp: 1.103 ± 0.13
3.058LeuTyr: 3.058 ± 0.217
0.0LeuXaa: 0.0 ± 0.0
Met
2.573MetAla: 2.573 ± 0.206
0.235MetCys: 0.235 ± 0.061
2.146MetAsp: 2.146 ± 0.169
1.411MetGlu: 1.411 ± 0.161
1.411MetPhe: 1.411 ± 0.129
1.691MetGly: 1.691 ± 0.165
0.691MetHis: 0.691 ± 0.1
1.72MetIle: 1.72 ± 0.151
1.955MetLys: 1.955 ± 0.207
2.323MetLeu: 2.323 ± 0.197
1.088MetMet: 1.088 ± 0.142
1.764MetAsn: 1.764 ± 0.191
1.426MetPro: 1.426 ± 0.138
1.47MetGln: 1.47 ± 0.168
1.764MetArg: 1.764 ± 0.172
2.396MetSer: 2.396 ± 0.172
1.941MetThr: 1.941 ± 0.153
1.985MetVal: 1.985 ± 0.185
0.338MetTrp: 0.338 ± 0.076
1.059MetTyr: 1.059 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
4.602AsnAla: 4.602 ± 0.317
0.309AsnCys: 0.309 ± 0.071
2.705AsnAsp: 2.705 ± 0.185
2.955AsnGlu: 2.955 ± 0.247
2.161AsnPhe: 2.161 ± 0.177
4.028AsnGly: 4.028 ± 0.297
0.912AsnHis: 0.912 ± 0.107
2.837AsnIle: 2.837 ± 0.213
2.72AsnLys: 2.72 ± 0.239
4.352AsnLeu: 4.352 ± 0.298
1.397AsnMet: 1.397 ± 0.15
2.705AsnAsn: 2.705 ± 0.253
2.661AsnPro: 2.661 ± 0.192
2.396AsnGln: 2.396 ± 0.187
2.308AsnArg: 2.308 ± 0.184
2.705AsnSer: 2.705 ± 0.189
3.176AsnThr: 3.176 ± 0.249
3.19AsnVal: 3.19 ± 0.206
0.779AsnTrp: 0.779 ± 0.111
1.558AsnTyr: 1.558 ± 0.125
0.0AsnXaa: 0.0 ± 0.0
Pro
3.823ProAla: 3.823 ± 0.468
0.279ProCys: 0.279 ± 0.065
3.293ProAsp: 3.293 ± 0.223
3.426ProGlu: 3.426 ± 0.246
1.72ProPhe: 1.72 ± 0.142
2.573ProGly: 2.573 ± 0.177
0.867ProHis: 0.867 ± 0.143
2.602ProIle: 2.602 ± 0.233
2.646ProLys: 2.646 ± 0.196
3.381ProLeu: 3.381 ± 0.272
1.161ProMet: 1.161 ± 0.12
2.323ProAsn: 2.323 ± 0.194
1.455ProPro: 1.455 ± 0.193
1.823ProGln: 1.823 ± 0.218
1.676ProArg: 1.676 ± 0.172
2.205ProSer: 2.205 ± 0.201
3.073ProThr: 3.073 ± 0.228
2.97ProVal: 2.97 ± 0.205
0.632ProTrp: 0.632 ± 0.105
1.426ProTyr: 1.426 ± 0.126
0.0ProXaa: 0.0 ± 0.0
Gln
3.999GlnAla: 3.999 ± 0.328
0.368GlnCys: 0.368 ± 0.064
2.426GlnAsp: 2.426 ± 0.2
2.22GlnGlu: 2.22 ± 0.191
1.911GlnPhe: 1.911 ± 0.16
2.911GlnGly: 2.911 ± 0.484
1.029GlnHis: 1.029 ± 0.129
2.514GlnIle: 2.514 ± 0.204
2.338GlnLys: 2.338 ± 0.223
3.528GlnLeu: 3.528 ± 0.245
1.426GlnMet: 1.426 ± 0.116
2.161GlnAsn: 2.161 ± 0.203
1.72GlnPro: 1.72 ± 0.219
2.029GlnGln: 2.029 ± 0.164
1.911GlnArg: 1.911 ± 0.173
2.279GlnSer: 2.279 ± 0.207
2.426GlnThr: 2.426 ± 0.221
2.793GlnVal: 2.793 ± 0.215
0.573GlnTrp: 0.573 ± 0.11
2.249GlnTyr: 2.249 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
3.543ArgAla: 3.543 ± 0.249
0.588ArgCys: 0.588 ± 0.1
3.911ArgAsp: 3.911 ± 0.471
3.396ArgGlu: 3.396 ± 0.386
2.323ArgPhe: 2.323 ± 0.184
2.94ArgGly: 2.94 ± 0.242
1.117ArgHis: 1.117 ± 0.135
3.029ArgIle: 3.029 ± 0.186
2.749ArgLys: 2.749 ± 0.219
4.087ArgLeu: 4.087 ± 0.269
1.529ArgMet: 1.529 ± 0.138
2.749ArgAsn: 2.749 ± 0.202
1.691ArgPro: 1.691 ± 0.165
2.088ArgGln: 2.088 ± 0.209
2.999ArgArg: 2.999 ± 0.37
2.602ArgSer: 2.602 ± 0.181
2.294ArgThr: 2.294 ± 0.197
3.176ArgVal: 3.176 ± 0.217
0.529ArgTrp: 0.529 ± 0.089
2.044ArgTyr: 2.044 ± 0.19
0.0ArgXaa: 0.0 ± 0.0
Ser
4.455SerAla: 4.455 ± 0.308
0.617SerCys: 0.617 ± 0.109
3.69SerAsp: 3.69 ± 0.222
3.279SerGlu: 3.279 ± 0.251
2.852SerPhe: 2.852 ± 0.224
4.366SerGly: 4.366 ± 0.283
1.308SerHis: 1.308 ± 0.157
3.808SerIle: 3.808 ± 0.228
3.279SerLys: 3.279 ± 0.249
4.808SerLeu: 4.808 ± 0.321
2.044SerMet: 2.044 ± 0.188
2.793SerAsn: 2.793 ± 0.274
2.323SerPro: 2.323 ± 0.19
2.338SerGln: 2.338 ± 0.185
2.69SerArg: 2.69 ± 0.214
3.705SerSer: 3.705 ± 0.284
3.44SerThr: 3.44 ± 0.226
3.764SerVal: 3.764 ± 0.224
0.72SerTrp: 0.72 ± 0.09
2.279SerTyr: 2.279 ± 0.163
0.0SerXaa: 0.0 ± 0.0
Thr
4.705ThrAla: 4.705 ± 0.368
0.441ThrCys: 0.441 ± 0.083
3.999ThrAsp: 3.999 ± 0.298
3.132ThrGlu: 3.132 ± 0.207
2.602ThrPhe: 2.602 ± 0.203
4.234ThrGly: 4.234 ± 0.541
1.014ThrHis: 1.014 ± 0.119
3.543ThrIle: 3.543 ± 0.249
3.499ThrLys: 3.499 ± 0.239
4.528ThrLeu: 4.528 ± 0.281
1.808ThrMet: 1.808 ± 0.167
3.176ThrAsn: 3.176 ± 0.283
3.132ThrPro: 3.132 ± 0.223
2.485ThrGln: 2.485 ± 0.207
2.661ThrArg: 2.661 ± 0.219
3.72ThrSer: 3.72 ± 0.296
3.925ThrThr: 3.925 ± 0.373
3.955ThrVal: 3.955 ± 0.32
0.765ThrTrp: 0.765 ± 0.137
2.249ThrTyr: 2.249 ± 0.187
0.0ThrXaa: 0.0 ± 0.0
Val
4.822ValAla: 4.822 ± 0.334
0.5ValCys: 0.5 ± 0.093
4.793ValAsp: 4.793 ± 0.28
4.484ValGlu: 4.484 ± 0.27
2.632ValPhe: 2.632 ± 0.205
4.146ValGly: 4.146 ± 0.325
1.176ValHis: 1.176 ± 0.132
3.484ValIle: 3.484 ± 0.265
4.425ValLys: 4.425 ± 0.238
5.19ValLeu: 5.19 ± 0.231
1.867ValMet: 1.867 ± 0.169
3.381ValAsn: 3.381 ± 0.201
2.837ValPro: 2.837 ± 0.248
2.837ValGln: 2.837 ± 0.195
3.029ValArg: 3.029 ± 0.23
3.778ValSer: 3.778 ± 0.259
4.161ValThr: 4.161 ± 0.319
4.205ValVal: 4.205 ± 0.283
0.735ValTrp: 0.735 ± 0.12
2.514ValTyr: 2.514 ± 0.171
0.0ValXaa: 0.0 ± 0.0
Trp
0.794TrpAla: 0.794 ± 0.126
0.176TrpCys: 0.176 ± 0.06
0.853TrpAsp: 0.853 ± 0.124
0.647TrpGlu: 0.647 ± 0.093
0.676TrpPhe: 0.676 ± 0.133
0.47TrpGly: 0.47 ± 0.083
0.279TrpHis: 0.279 ± 0.067
0.867TrpIle: 0.867 ± 0.11
0.882TrpLys: 0.882 ± 0.111
1.411TrpLeu: 1.411 ± 0.172
0.426TrpMet: 0.426 ± 0.078
0.573TrpAsn: 0.573 ± 0.095
0.456TrpPro: 0.456 ± 0.077
0.588TrpGln: 0.588 ± 0.098
0.617TrpArg: 0.617 ± 0.091
0.735TrpSer: 0.735 ± 0.129
0.867TrpThr: 0.867 ± 0.113
0.823TrpVal: 0.823 ± 0.106
0.103TrpTrp: 0.103 ± 0.036
0.603TrpTyr: 0.603 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.764TyrAla: 2.764 ± 0.21
0.382TyrCys: 0.382 ± 0.08
2.837TyrAsp: 2.837 ± 0.282
2.22TyrGlu: 2.22 ± 0.191
1.838TyrPhe: 1.838 ± 0.165
2.793TyrGly: 2.793 ± 0.229
0.838TyrHis: 0.838 ± 0.109
1.985TyrIle: 1.985 ± 0.194
1.985TyrLys: 1.985 ± 0.184
3.264TyrLeu: 3.264 ± 0.216
1.323TyrMet: 1.323 ± 0.153
1.941TyrAsn: 1.941 ± 0.161
1.999TyrPro: 1.999 ± 0.206
1.588TyrGln: 1.588 ± 0.147
2.279TyrArg: 2.279 ± 0.184
2.249TyrSer: 2.249 ± 0.203
2.22TyrThr: 2.22 ± 0.178
2.735TyrVal: 2.735 ± 0.22
0.647TyrTrp: 0.647 ± 0.093
1.823TyrTyr: 1.823 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 237 proteins (68019 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski