Klebsiella phage vB_KpnM_KpV79

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; Klebsiella virus KpV52

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291LBI7|A0A291LBI7_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_KpV79 OX=2041212 GN=kpv79_38 PE=4 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.83YY4 pKa = 10.02IWTTSIPVEE13 pKa = 3.95RR14 pKa = 11.84SIGGVVIDD22 pKa = 4.01TEE24 pKa = 4.46YY25 pKa = 11.4VDD27 pKa = 4.86NEE29 pKa = 4.2VEE31 pKa = 4.12LFIPEE36 pKa = 3.88VTVRR40 pKa = 11.84ANGVAVCPVYY50 pKa = 10.24MSYY53 pKa = 11.67NNTQQLIKK61 pKa = 10.71FMEE64 pKa = 4.89FSSGGNDD71 pKa = 3.5PFASAYY77 pKa = 9.39EE78 pKa = 4.22HH79 pKa = 6.88LLSDD83 pKa = 3.75PQFEE87 pKa = 4.5GVRR90 pKa = 11.84VEE92 pKa = 4.91AEE94 pKa = 3.7

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291LBG7|A0A291LBG7_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_KpV79 OX=2041212 GN=kpv79_19 PE=4 SV=1
MM1 pKa = 7.49GSLVEE6 pKa = 5.26DD7 pKa = 4.22ILRR10 pKa = 11.84HH11 pKa = 5.25VPTKK15 pKa = 8.54QWRR18 pKa = 11.84KK19 pKa = 8.12KK20 pKa = 9.44CDD22 pKa = 3.71RR23 pKa = 11.84IASRR27 pKa = 11.84EE28 pKa = 3.89LHH30 pKa = 6.12LARR33 pKa = 11.84VPGIHH38 pKa = 6.4HH39 pKa = 7.56RR40 pKa = 11.84YY41 pKa = 8.84GKK43 pKa = 9.13WEE45 pKa = 3.77AVVFYY50 pKa = 11.0RR51 pKa = 11.84NVTLTIGCFNTPHH64 pKa = 7.12RR65 pKa = 11.84ALLARR70 pKa = 11.84KK71 pKa = 8.75LWYY74 pKa = 9.31FWRR77 pKa = 11.84EE78 pKa = 3.56RR79 pKa = 11.84GFTPQEE85 pKa = 3.75IPKK88 pKa = 9.88GPKK91 pKa = 8.95RR92 pKa = 11.84EE93 pKa = 4.08PYY95 pKa = 10.14SRR97 pKa = 11.84SS98 pKa = 3.19

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14722

31

772

196.3

21.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.362 ± 0.384

1.474 ± 0.177

6.215 ± 0.215

5.726 ± 0.307

3.423 ± 0.193

7.377 ± 0.235

1.895 ± 0.174

5.869 ± 0.172

5.448 ± 0.335

7.356 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.166

4.694 ± 0.227

4.123 ± 0.228

4.035 ± 0.23

5.353 ± 0.237

6.392 ± 0.31

6.915 ± 0.318

7.411 ± 0.238

1.589 ± 0.101

3.702 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski