Ancylomarina euxinus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinifilaceae; Ancylomarina

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A425Y4A8|A0A425Y4A8_9BACT ATP phosphoribosyltransferase OS=Ancylomarina euxinus OX=2283627 GN=hisG PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.33KK3 pKa = 9.48ILYY6 pKa = 9.54FILLLVVAVSCSPDD20 pKa = 3.32EE21 pKa = 4.22YY22 pKa = 10.68STPEE26 pKa = 4.01NLTAEE31 pKa = 4.33QIDD34 pKa = 3.65WGYY37 pKa = 7.84TATDD41 pKa = 3.09ITNEE45 pKa = 3.67YY46 pKa = 8.73TLYY49 pKa = 10.98NNTPGVSSTWDD60 pKa = 3.23FGNGVTQKK68 pKa = 10.41GSSVTAQYY76 pKa = 10.31TFAGTYY82 pKa = 6.88TVKK85 pKa = 10.02LTVISQGGVIILEE98 pKa = 4.27DD99 pKa = 4.47EE100 pKa = 4.22IVTTVDD106 pKa = 3.05NPSFLSGYY114 pKa = 9.86PYY116 pKa = 10.66DD117 pKa = 3.84QLVGAGEE124 pKa = 4.26QVWAVDD130 pKa = 4.86GYY132 pKa = 11.32SKK134 pKa = 11.13GAFGLGPTLANPTEE148 pKa = 3.88WHH150 pKa = 6.79ADD152 pKa = 3.2AKK154 pKa = 10.59GARR157 pKa = 11.84LDD159 pKa = 3.4KK160 pKa = 11.05GLYY163 pKa = 9.86DD164 pKa = 4.67DD165 pKa = 5.65RR166 pKa = 11.84FTFKK170 pKa = 9.53ITEE173 pKa = 4.21SGLTLTQLTNGDD185 pKa = 3.65VYY187 pKa = 11.76ANGGWAADD195 pKa = 3.85LGSTDD200 pKa = 3.3GHH202 pKa = 5.81QEE204 pKa = 3.55PDD206 pKa = 3.17GGDD209 pKa = 3.56FIMPHH214 pKa = 5.68TGGDD218 pKa = 4.14FVCTIAGEE226 pKa = 4.29KK227 pKa = 9.49LTVTGGGFLGYY238 pKa = 10.14YY239 pKa = 10.18AGAHH243 pKa = 5.77EE244 pKa = 4.49YY245 pKa = 10.59EE246 pKa = 5.21IITLTEE252 pKa = 4.38DD253 pKa = 3.41LLEE256 pKa = 4.19VAFWDD261 pKa = 4.19TNANFYY267 pKa = 10.22WFTRR271 pKa = 11.84FAPVDD276 pKa = 3.46KK277 pKa = 9.73LTPEE281 pKa = 4.18PEE283 pKa = 4.11PVVKK287 pKa = 10.25EE288 pKa = 4.21LEE290 pKa = 4.45SNDD293 pKa = 3.32IADD296 pKa = 4.08DD297 pKa = 3.99FEE299 pKa = 6.54GNGNIIWDD307 pKa = 3.71TAAIDD312 pKa = 3.93GFDD315 pKa = 4.45IIDD318 pKa = 3.85NFAPSDD324 pKa = 3.89VNSSDD329 pKa = 5.57NIIKK333 pKa = 8.28YY334 pKa = 10.15QKK336 pKa = 10.76GAGEE340 pKa = 4.19WSSVLTVLDD349 pKa = 4.15YY350 pKa = 10.96IIDD353 pKa = 4.24LSTRR357 pKa = 11.84NQFTMKK363 pKa = 10.29VFVPAFNDD371 pKa = 3.33YY372 pKa = 7.83TTVCDD377 pKa = 5.58PGTPWIADD385 pKa = 3.64HH386 pKa = 6.42NLKK389 pKa = 9.9PQIDD393 pKa = 4.01VKK395 pKa = 11.17LQDD398 pKa = 3.48SSLGGNAWQTQQVRR412 pKa = 11.84SHH414 pKa = 6.49TLTEE418 pKa = 4.12AQFGTWVEE426 pKa = 4.1LTFDD430 pKa = 3.69YY431 pKa = 11.26SDD433 pKa = 3.23VSDD436 pKa = 5.05RR437 pKa = 11.84VDD439 pKa = 3.89FDD441 pKa = 4.08QIVIQLGAEE450 pKa = 4.28GHH452 pKa = 6.42CNSGLFYY459 pKa = 10.34IDD461 pKa = 5.64DD462 pKa = 4.1FQLLPP467 pKa = 4.16

Molecular weight: 51.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A425Y5P2|A0A425Y5P2_9BACT Type IX secretion system membrane protein PorP/SprF OS=Ancylomarina euxinus OX=2283627 GN=DWB61_04540 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 10.23HH16 pKa = 3.88GFRR19 pKa = 11.84DD20 pKa = 3.68KK21 pKa = 10.42MASANGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.62LSVSSEE47 pKa = 3.87RR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 5.76KK51 pKa = 10.9AA52 pKa = 2.86

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3457

0

3457

1249026

23

3318

361.3

40.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.308 ± 0.039

0.988 ± 0.017

5.708 ± 0.031

6.75 ± 0.036

5.061 ± 0.03

6.311 ± 0.042

1.84 ± 0.016

8.113 ± 0.036

7.878 ± 0.042

9.622 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.022

5.764 ± 0.035

3.21 ± 0.021

3.366 ± 0.021

3.699 ± 0.025

6.856 ± 0.036

5.003 ± 0.033

5.961 ± 0.036

1.049 ± 0.012

4.03 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski