[Pantoea] beijingensis
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3521 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A443I8S4|A0A443I8S4_9GAMM Pyruvate dehydrogenase E1 component OS=[Pantoea] beijingensis OX=1324864 GN=aceE PE=4 SV=1
MM1 pKa = 7.37 QKK3 pKa = 10.35 YY4 pKa = 9.92 CDD6 pKa = 3.6 LVRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.59 YY12 pKa = 11.11 AEE14 pKa = 4.18 IGSGDD19 pKa = 3.94 LGYY22 pKa = 11.08 VPDD25 pKa = 6.31 AIGCVLNALDD35 pKa = 5.26 RR36 pKa = 11.84 IAANSEE42 pKa = 3.83 LDD44 pKa = 3.45 SSIRR48 pKa = 11.84 EE49 pKa = 3.88 QAAYY53 pKa = 9.92 AAANLLVSDD62 pKa = 4.7 YY63 pKa = 11.37 VDD65 pKa = 3.35 EE66 pKa = 4.49
Molecular weight: 7.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.955
IPC2_protein 4.291
IPC_protein 4.164
Toseland 3.961
ProMoST 4.24
Dawson 4.139
Bjellqvist 4.393
Wikipedia 4.088
Rodwell 3.986
Grimsley 3.872
Solomon 4.126
Lehninger 4.088
Nozaki 4.266
DTASelect 4.482
Thurlkill 4.024
EMBOSS 4.088
Sillero 4.279
Patrickios 1.977
IPC_peptide 4.126
IPC2_peptide 4.253
IPC2.peptide.svr19 4.211
Protein with the highest isoelectric point:
>tr|A0A443IH45|A0A443IH45_9GAMM Uncharacterized protein OS=[Pantoea] beijingensis OX=1324864 GN=ED28_03215 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LTVSKK46 pKa = 11.03
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3521
0
3521
1141336
35
2613
324.2
35.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.601 ± 0.046
1.024 ± 0.013
5.249 ± 0.035
5.527 ± 0.041
3.864 ± 0.028
7.375 ± 0.041
2.342 ± 0.021
6.019 ± 0.035
4.127 ± 0.035
11.037 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.744 ± 0.021
3.811 ± 0.03
4.416 ± 0.027
4.64 ± 0.045
5.772 ± 0.034
6.151 ± 0.03
5.253 ± 0.026
6.851 ± 0.029
1.463 ± 0.019
2.734 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here