Gordonia phage Fairfaxidum
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6T6G6|A0A4D6T6G6_9CAUD Uncharacterized protein OS=Gordonia phage Fairfaxidum OX=2572526 GN=38 PE=4 SV=1
MM1 pKa = 7.45 FIEE4 pKa = 4.8 FVLRR8 pKa = 11.84 NAGFCFVLACCIGAGGYY25 pKa = 7.17 TLYY28 pKa = 10.87 DD29 pKa = 3.79 LSRR32 pKa = 11.84 GAFARR37 pKa = 11.84 DD38 pKa = 4.15 DD39 pKa = 3.57 IDD41 pKa = 4.1 ALTVHH46 pKa = 6.67 KK47 pKa = 11.05 GEE49 pKa = 4.29 IVEE52 pKa = 4.1 VDD54 pKa = 3.1 QQ55 pKa = 5.45
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.14
IPC2_protein 4.533
IPC_protein 4.304
Toseland 4.126
ProMoST 4.469
Dawson 4.291
Bjellqvist 4.457
Wikipedia 4.228
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.24
Nozaki 4.431
DTASelect 4.622
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.431
Patrickios 2.015
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.348
Protein with the highest isoelectric point:
>tr|A0A4D6T7N7|A0A4D6T7N7_9CAUD Lysin A OS=Gordonia phage Fairfaxidum OX=2572526 GN=20 PE=4 SV=1
MM1 pKa = 6.8 TTPSPAMTIAAAAAEE16 pKa = 4.29 PKK18 pKa = 10.0 KK19 pKa = 11.06 SKK21 pKa = 10.46 ARR23 pKa = 11.84 PDD25 pKa = 3.46 RR26 pKa = 11.84 DD27 pKa = 3.22 LVVYY31 pKa = 7.56 STKK34 pKa = 10.3 SGRR37 pKa = 11.84 WHH39 pKa = 6.39 WKK41 pKa = 8.26 VLSPNGQVVQARR53 pKa = 11.84 SGPDD57 pKa = 3.13 GYY59 pKa = 10.77 RR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 DD63 pKa = 3.69 DD64 pKa = 3.31 ATVAARR70 pKa = 11.84 RR71 pKa = 11.84 FLTSIGSSAVRR82 pKa = 11.84 LIVHH86 pKa = 6.86 KK87 pKa = 10.85 ADD89 pKa = 3.25 GTIDD93 pKa = 3.85 DD94 pKa = 4.71 RR95 pKa = 11.84 GQII98 pKa = 3.71
Molecular weight: 10.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.706
IPC_protein 10.555
Toseland 10.877
ProMoST 10.599
Dawson 10.935
Bjellqvist 10.643
Wikipedia 11.155
Rodwell 11.14
Grimsley 10.965
Solomon 11.096
Lehninger 11.067
Nozaki 10.833
DTASelect 10.643
Thurlkill 10.862
EMBOSS 11.272
Sillero 10.877
Patrickios 10.906
IPC_peptide 11.111
IPC2_peptide 9.399
IPC2.peptide.svr19 8.714
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
81
0
81
16210
42
1873
200.1
21.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.017 ± 0.538
0.802 ± 0.168
7.033 ± 0.314
5.645 ± 0.248
2.677 ± 0.185
8.476 ± 0.411
2.067 ± 0.186
4.503 ± 0.201
3.362 ± 0.319
7.736 ± 0.242
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.054 ± 0.193
2.684 ± 0.141
5.731 ± 0.253
3.455 ± 0.156
7.452 ± 0.421
5.423 ± 0.244
6.595 ± 0.389
7.958 ± 0.256
2.11 ± 0.16
2.221 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here