Tortoise microvirus 37
Average proteome isoelectric point is 7.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5X0|A0A4P8W5X0_9VIRU Uncharacterized protein OS=Tortoise microvirus 37 OX=2583140 PE=4 SV=1
MM1 pKa = 7.6 NYY3 pKa = 9.55 VQLEE7 pKa = 4.22 HH8 pKa = 7.48 LSDD11 pKa = 3.34 WVKK14 pKa = 10.42 TMGADD19 pKa = 3.18 QFFNVGDD26 pKa = 3.59 QPRR29 pKa = 11.84 AVVFRR34 pKa = 11.84 VNNPLYY40 pKa = 10.41 PMALEE45 pKa = 4.0 ITADD49 pKa = 3.44 SNYY52 pKa = 9.13 WLLPFGYY59 pKa = 9.71 DD60 pKa = 5.02 DD61 pKa = 4.77 NGQPLPDD68 pKa = 4.76 PEE70 pKa = 4.76 SGEE73 pKa = 4.19 IPSLPGEE80 pKa = 4.73 GPTEE84 pKa = 3.65 RR85 pKa = 11.84 ALARR89 pKa = 11.84 LEE91 pKa = 4.11 PGMNEE96 pKa = 4.03 VAFTLPYY103 pKa = 8.45 PALVKK108 pKa = 9.62 FYY110 pKa = 10.58 RR111 pKa = 11.84 IGPDD115 pKa = 3.42 GSVQPPGSQFQVRR128 pKa = 11.84 RR129 pKa = 11.84 YY130 pKa = 10.14 DD131 pKa = 3.71 LSQLVQEE138 pKa = 4.79 SGEE141 pKa = 3.87 PSYY144 pKa = 11.46 VQIGSRR150 pKa = 11.84 PTLDD154 pKa = 3.4 PNYY157 pKa = 10.38 QRR159 pKa = 11.84 MEE161 pKa = 3.64 MLMRR165 pKa = 11.84 WNEE168 pKa = 3.91 VQRR171 pKa = 11.84 NRR173 pKa = 11.84 AFQEE177 pKa = 3.56 EE178 pKa = 4.45 LAALRR183 pKa = 11.84 DD184 pKa = 3.81 EE185 pKa = 5.1 FGNPTGTSTDD195 pKa = 2.62 VDD197 pKa = 3.6 IPVVEE202 pKa = 4.29 DD203 pKa = 3.6 TVPGGGAA210 pKa = 3.07
Molecular weight: 23.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.362
IPC2_protein 4.291
IPC_protein 4.228
Toseland 4.05
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.05
Grimsley 3.961
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.457
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.329
Patrickios 3.668
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.264
Protein with the highest isoelectric point:
>tr|A0A4P8W685|A0A4P8W685_9VIRU Replication initiation protein OS=Tortoise microvirus 37 OX=2583140 PE=4 SV=1
MM1 pKa = 7.34 EE2 pKa = 5.42 AVSPPPLPKK11 pKa = 10.04 KK12 pKa = 10.65 ALTHH16 pKa = 6.56 ADD18 pKa = 3.32 AAAGLSDD25 pKa = 3.41 TVYY28 pKa = 10.37 IASADD33 pKa = 3.68 YY34 pKa = 10.49 RR35 pKa = 11.84 RR36 pKa = 11.84 LAIRR40 pKa = 11.84 TPRR43 pKa = 11.84 EE44 pKa = 3.5 GAEE47 pKa = 3.61 PDD49 pKa = 2.95 IVRR52 pKa = 11.84 FYY54 pKa = 11.51 LAFQKK59 pKa = 11.01 DD60 pKa = 3.43 LVARR64 pKa = 11.84 GFPFFATEE72 pKa = 3.97 FLRR75 pKa = 11.84 GRR77 pKa = 11.84 DD78 pKa = 3.62 RR79 pKa = 11.84 QQALYY84 pKa = 10.68 NAGNSKK90 pKa = 10.73 AVFGRR95 pKa = 11.84 SPHH98 pKa = 5.44 NFGMAVDD105 pKa = 4.09 IVHH108 pKa = 7.33 CKK110 pKa = 10.3 RR111 pKa = 11.84 LWDD114 pKa = 4.38 LKK116 pKa = 8.2 PQEE119 pKa = 3.93 WALIGLLGKK128 pKa = 9.8 EE129 pKa = 4.28 AARR132 pKa = 11.84 KK133 pKa = 9.39 QSIKK137 pKa = 10.45 IEE139 pKa = 3.96 WGGDD143 pKa = 2.46 WFGRR147 pKa = 11.84 GKK149 pKa = 10.56 RR150 pKa = 11.84 RR151 pKa = 11.84 TSPGQVGWDD160 pKa = 3.49 PAHH163 pKa = 7.09 WEE165 pKa = 3.78 LADD168 pKa = 3.21 WAKK171 pKa = 10.59 RR172 pKa = 11.84 IRR174 pKa = 11.84 GLL176 pKa = 3.91
Molecular weight: 19.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.129
IPC2_protein 9.238
IPC_protein 9.326
Toseland 10.16
ProMoST 9.75
Dawson 10.292
Bjellqvist 9.94
Wikipedia 10.438
Rodwell 10.687
Grimsley 10.35
Solomon 10.335
Lehninger 10.321
Nozaki 10.131
DTASelect 9.926
Thurlkill 10.175
EMBOSS 10.54
Sillero 10.204
Patrickios 10.409
IPC_peptide 10.35
IPC2_peptide 8.492
IPC2.peptide.svr19 8.314
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1789
68
542
255.6
28.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.496 ± 0.677
0.894 ± 0.419
6.26 ± 0.405
5.59 ± 0.512
3.969 ± 0.28
8.44 ± 1.057
2.124 ± 0.408
4.472 ± 0.527
4.863 ± 0.586
7.826 ± 0.36
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.851 ± 0.468
3.801 ± 0.841
6.372 ± 0.657
3.913 ± 0.482
7.211 ± 0.577
4.975 ± 0.242
5.646 ± 0.582
6.875 ± 0.6
2.18 ± 0.407
3.242 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here