Thermobacillus composti (strain DSM 18247 / JCM 13945 / KWC4)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Thermobacillus; Thermobacillus composti

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3802 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0E9H3|L0E9H3_THECK Nucleoside-diphosphate-sugar epimerase OS=Thermobacillus composti (strain DSM 18247 / JCM 13945 / KWC4) OX=717605 GN=Theco_0170 PE=4 SV=1
MM1 pKa = 6.98YY2 pKa = 9.6CVCKK6 pKa = 10.35EE7 pKa = 4.08HH8 pKa = 7.52VEE10 pKa = 3.94LAIDD14 pKa = 3.97RR15 pKa = 11.84FVDD18 pKa = 4.09EE19 pKa = 5.56YY20 pKa = 11.33EE21 pKa = 4.46DD22 pKa = 5.15APDD25 pKa = 5.09LVDD28 pKa = 4.4LNKK31 pKa = 9.79TAFAEE36 pKa = 4.3WEE38 pKa = 4.24PPATCDD44 pKa = 2.9MCEE47 pKa = 4.13RR48 pKa = 11.84PPEE51 pKa = 4.1VLVVV55 pKa = 3.57

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0EJA0|L0EJA0_THECK 50S ribosomal protein L9 OS=Thermobacillus composti (strain DSM 18247 / JCM 13945 / KWC4) OX=717605 GN=rplI PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 8.7PTFKK6 pKa = 10.81PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 9.11VLAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.23KK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3802

0

3802

1222205

26

2828

321.5

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.133 ± 0.063

0.806 ± 0.011

5.345 ± 0.029

7.037 ± 0.042

3.874 ± 0.029

8.074 ± 0.041

2.05 ± 0.019

5.957 ± 0.033

4.266 ± 0.034

10.102 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.018

3.009 ± 0.028

4.553 ± 0.025

3.105 ± 0.023

7.233 ± 0.05

5.186 ± 0.03

4.901 ± 0.034

7.159 ± 0.029

1.373 ± 0.018

3.184 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski