Fowl aviadenovirus E
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E9KLB4|E9KLB4_9ADEN ORF32 OS=Fowl aviadenovirus E OX=190065 PE=4 SV=1
MM1 pKa = 7.12 ATSTPHH7 pKa = 6.67 AFSFGQIGSRR17 pKa = 11.84 KK18 pKa = 9.34 RR19 pKa = 11.84 PAGGDD24 pKa = 3.67 GEE26 pKa = 4.79 RR27 pKa = 11.84 DD28 pKa = 3.22 ASKK31 pKa = 10.42 VPKK34 pKa = 8.97 MQTPAPSATANGNDD48 pKa = 4.13 EE49 pKa = 5.04 LDD51 pKa = 3.4 LVYY54 pKa = 10.27 PFWLQNGSTGGGGGGGGSGGNPSLNPPFLDD84 pKa = 3.61 PNGPLAVQNNLLKK97 pKa = 11.07 VNTAAPITVANKK109 pKa = 10.35 ALTLAYY115 pKa = 9.96 EE116 pKa = 4.36 PDD118 pKa = 3.78 SLEE121 pKa = 4.31 LTNQQQLAVKK131 pKa = 9.52 IDD133 pKa = 3.54 PEE135 pKa = 5.08 GPLKK139 pKa = 10.43 ATTEE143 pKa = 4.8 GIQLSVDD150 pKa = 3.45 PTTLEE155 pKa = 3.71 VDD157 pKa = 3.65 DD158 pKa = 5.05 VDD160 pKa = 3.82 WEE162 pKa = 4.5 LTVKK166 pKa = 10.64 LDD168 pKa = 3.77 PDD170 pKa = 4.22 GPLDD174 pKa = 3.87 SSATGITVRR183 pKa = 11.84 VDD185 pKa = 3.12 EE186 pKa = 4.58 TLLIEE191 pKa = 5.21 DD192 pKa = 4.14 VGSGQGKK199 pKa = 9.0 EE200 pKa = 3.96 LGVNLNPTGPITADD214 pKa = 3.45 DD215 pKa = 3.66 QGLDD219 pKa = 4.5 LEE221 pKa = 5.33 IDD223 pKa = 3.64 NQTLKK228 pKa = 10.94 VNSVTGGGVLAVQLKK243 pKa = 9.59 SQGGLTAQTDD253 pKa = 5.39 GIQVNTQNSITVTNGALDD271 pKa = 3.61 VKK273 pKa = 10.68 VAANGPLEE281 pKa = 4.23 STDD284 pKa = 3.14 TGLTLNYY291 pKa = 10.33 DD292 pKa = 3.79 PGDD295 pKa = 3.62 FTVNAGTLSIIRR307 pKa = 11.84 DD308 pKa = 3.59 PALVANAYY316 pKa = 7.16 LTSGASTLQQFTAKK330 pKa = 10.14 SEE332 pKa = 4.06 NSSQFSFPCAYY343 pKa = 10.45 YY344 pKa = 9.99 LQQWLSDD351 pKa = 3.64 GLVLSSLYY359 pKa = 10.79 LKK361 pKa = 10.5 LDD363 pKa = 3.56 RR364 pKa = 11.84 AQFTNMPTGANYY376 pKa = 10.13 QNARR380 pKa = 11.84 YY381 pKa = 7.53 FTFWVGAGTSFNLSTLTEE399 pKa = 4.02 PTITPNTTQWNAFAPALDD417 pKa = 4.12 YY418 pKa = 11.51 SGAPPFIYY426 pKa = 10.17 DD427 pKa = 3.38 ASSVVTIYY435 pKa = 10.55 FEE437 pKa = 4.19 PTSGRR442 pKa = 11.84 LEE444 pKa = 4.22 SYY446 pKa = 10.98 LPVLTDD452 pKa = 3.12 NWSQTYY458 pKa = 10.61 NPGTVTLCVKK468 pKa = 8.17 TVRR471 pKa = 11.84 VQLRR475 pKa = 11.84 SQGTFSTLVCYY486 pKa = 10.21 NFRR489 pKa = 11.84 CQNTGIFNSNATAGTMTLGPIFFSCPALSTANAPP523 pKa = 3.63
Molecular weight: 55.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.215
IPC2_protein 4.177
IPC_protein 4.177
Toseland 3.961
ProMoST 4.329
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.075
Rodwell 3.999
Grimsley 3.872
Solomon 4.151
Lehninger 4.113
Nozaki 4.266
DTASelect 4.507
Thurlkill 3.999
EMBOSS 4.088
Sillero 4.291
Patrickios 3.427
IPC_peptide 4.151
IPC2_peptide 4.279
IPC2.peptide.svr19 4.218
Protein with the highest isoelectric point:
>tr|E9KLA4|E9KLA4_9ADEN Core protein pX OS=Fowl aviadenovirus E OX=190065 PE=4 SV=1
MM1 pKa = 7.68 SILISPNDD9 pKa = 3.25 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGIRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 LTLRR36 pKa = 11.84 TLLGLGTVSRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 GGRR52 pKa = 11.84 SSRR55 pKa = 11.84 RR56 pKa = 11.84 SSRR59 pKa = 11.84 PASTTTRR66 pKa = 11.84 LMVVRR71 pKa = 11.84 TSRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 3.32
Molecular weight: 9.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.296
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
1
46
12659
54
1226
275.2
30.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.018 ± 0.454
1.88 ± 0.233
5.419 ± 0.378
5.79 ± 0.436
4.139 ± 0.216
6.841 ± 0.398
2.401 ± 0.211
3.507 ± 0.15
3.247 ± 0.335
8.966 ± 0.341
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.17
3.855 ± 0.4
6.896 ± 0.393
3.705 ± 0.233
8.042 ± 0.668
7.102 ± 0.55
6.019 ± 0.388
6.636 ± 0.345
1.382 ± 0.145
3.744 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here