Parietaria mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6RK14|Q6RK14_9BROM ATP-dependent helicase OS=Parietaria mottle virus OX=64958 PE=3 SV=1
MM1 pKa = 7.42DD2 pKa = 4.85QIRR5 pKa = 11.84INLIDD10 pKa = 3.85SFLSSRR16 pKa = 11.84LCFNATFGVYY26 pKa = 9.97HH27 pKa = 7.73DD28 pKa = 6.31DD29 pKa = 3.44YY30 pKa = 10.91MEE32 pKa = 4.05WVKK35 pKa = 10.95LFLFKK40 pKa = 10.7HH41 pKa = 5.64VIEE44 pKa = 4.61HH45 pKa = 5.19TAKK48 pKa = 10.22FDD50 pKa = 3.39FATIHH55 pKa = 5.5MTLEE59 pKa = 4.03MVLCRR64 pKa = 11.84LVPGLGDD71 pKa = 3.31ALYY74 pKa = 9.94PEE76 pKa = 5.17EE77 pKa = 4.19EE78 pKa = 4.37NEE80 pKa = 4.03VDD82 pKa = 3.52GLSSRR87 pKa = 11.84EE88 pKa = 3.48IDD90 pKa = 3.94PFYY93 pKa = 11.13LPYY96 pKa = 10.75DD97 pKa = 4.42DD98 pKa = 6.49LDD100 pKa = 3.52VDD102 pKa = 3.87YY103 pKa = 9.26TALCRR108 pKa = 11.84EE109 pKa = 4.42RR110 pKa = 11.84DD111 pKa = 3.71DD112 pKa = 5.27SIPTIPPEE120 pKa = 4.47DD121 pKa = 4.05EE122 pKa = 4.25LSVIVKK128 pKa = 10.05NMTYY132 pKa = 9.91IPEE135 pKa = 4.38GTSWGDD141 pKa = 3.23VSDD144 pKa = 5.25SSFQEE149 pKa = 4.01HH150 pKa = 6.79LEE152 pKa = 4.34EE153 pKa = 4.35IQPVPLGSDD162 pKa = 3.25VGHH165 pKa = 7.18TIPNEE170 pKa = 4.31HH171 pKa = 7.05IPFDD175 pKa = 5.07DD176 pKa = 5.19DD177 pKa = 3.8GQLVDD182 pKa = 4.54VQWSDD187 pKa = 4.77AIPSLATDD195 pKa = 3.85VTCDD199 pKa = 3.04ADD201 pKa = 3.62YY202 pKa = 10.99RR203 pKa = 11.84EE204 pKa = 4.92CGFVSYY210 pKa = 11.05SSGSQHH216 pKa = 6.44SSRR219 pKa = 11.84WKK221 pKa = 9.83PKK223 pKa = 9.11VSQVKK228 pKa = 9.2PDD230 pKa = 3.64PSIIQSAIDD239 pKa = 3.46EE240 pKa = 5.0LFPHH244 pKa = 6.27HH245 pKa = 6.93HH246 pKa = 5.96SVEE249 pKa = 4.0DD250 pKa = 3.55RR251 pKa = 11.84FFQEE255 pKa = 3.47WVEE258 pKa = 3.99THH260 pKa = 7.34DD261 pKa = 4.07IDD263 pKa = 6.38LEE265 pKa = 4.29VSDD268 pKa = 5.63CNLDD272 pKa = 3.29MSVFNDD278 pKa = 3.23WSKK281 pKa = 10.37GTDD284 pKa = 3.19TRR286 pKa = 11.84LVPQMCVGGLSHH298 pKa = 7.2RR299 pKa = 11.84VPTQRR304 pKa = 11.84EE305 pKa = 3.62ALLAIKK311 pKa = 9.37KK312 pKa = 10.3RR313 pKa = 11.84NMNVPEE319 pKa = 4.35LQSQFDD325 pKa = 3.99HH326 pKa = 6.91EE327 pKa = 5.03DD328 pKa = 3.34VLNRR332 pKa = 11.84CVNRR336 pKa = 11.84FLTHH340 pKa = 6.03VVDD343 pKa = 3.8KK344 pKa = 10.85TRR346 pKa = 11.84LGKK349 pKa = 10.54LMPISGEE356 pKa = 3.65EE357 pKa = 4.14VYY359 pKa = 10.74FFNQYY364 pKa = 10.71LEE366 pKa = 4.39NKK368 pKa = 9.44NPPLSEE374 pKa = 4.1YY375 pKa = 10.32KK376 pKa = 10.97GPVPLLALDD385 pKa = 4.8KK386 pKa = 10.0YY387 pKa = 9.42MHH389 pKa = 6.63MIKK392 pKa = 7.75TTLKK396 pKa = 10.23PVEE399 pKa = 4.94DD400 pKa = 4.74DD401 pKa = 3.36SLHH404 pKa = 6.45IEE406 pKa = 4.21RR407 pKa = 11.84PVPATITYY415 pKa = 8.29HH416 pKa = 5.88KK417 pKa = 9.69KK418 pKa = 9.51GVVMMTSPYY427 pKa = 9.61FLCAMVRR434 pKa = 11.84LLYY437 pKa = 10.17VLKK440 pKa = 10.61SKK442 pKa = 10.77VFIPTGKK449 pKa = 9.24YY450 pKa = 9.14HH451 pKa = 7.24QIFQMDD457 pKa = 4.13PVRR460 pKa = 11.84LKK462 pKa = 10.4NSKK465 pKa = 9.6FFKK468 pKa = 10.62EE469 pKa = 3.42IDD471 pKa = 3.37FSKK474 pKa = 10.51FDD476 pKa = 3.37KK477 pKa = 11.04SQGRR481 pKa = 11.84LHH483 pKa = 7.08HH484 pKa = 7.0DD485 pKa = 3.21VQLRR489 pKa = 11.84LFLHH493 pKa = 6.66LGIPQHH499 pKa = 6.96FVDD502 pKa = 3.62TWFNAHH508 pKa = 5.96EE509 pKa = 4.3RR510 pKa = 11.84SHH512 pKa = 7.94IRR514 pKa = 11.84DD515 pKa = 3.64RR516 pKa = 11.84DD517 pKa = 3.73CGIGFSVDD525 pKa = 3.25YY526 pKa = 10.46QRR528 pKa = 11.84RR529 pKa = 11.84TGDD532 pKa = 2.74ACTYY536 pKa = 10.46LGNTLVTLSVLSYY549 pKa = 11.1VYY551 pKa = 10.62DD552 pKa = 3.92LSKK555 pKa = 10.95PDD557 pKa = 3.62ILFVAASGDD566 pKa = 3.57DD567 pKa = 3.75SLIGSLNPLPRR578 pKa = 11.84DD579 pKa = 3.69KK580 pKa = 11.33EE581 pKa = 4.21DD582 pKa = 3.51LCVSLFNLEE591 pKa = 3.88TKK593 pKa = 10.47FPHH596 pKa = 5.74NQPFLCSKK604 pKa = 9.95FLLVVEE610 pKa = 5.04CDD612 pKa = 3.71DD613 pKa = 3.73GTEE616 pKa = 3.98EE617 pKa = 4.32VLAVPNPLKK626 pKa = 10.32ILQKK630 pKa = 9.95MGPKK634 pKa = 9.47NLQVTVLDD642 pKa = 4.97DD643 pKa = 4.31YY644 pKa = 11.44YY645 pKa = 11.6QSLCDD650 pKa = 3.68ILWVFEE656 pKa = 4.54DD657 pKa = 3.8ADD659 pKa = 3.3ICRR662 pKa = 11.84RR663 pKa = 11.84VAQLAEE669 pKa = 3.72YY670 pKa = 10.1RR671 pKa = 11.84QFKK674 pKa = 9.91GKK676 pKa = 9.92RR677 pKa = 11.84SCVFLEE683 pKa = 4.11SALLSLPSLVANRR696 pKa = 11.84LKK698 pKa = 10.85FIRR701 pKa = 11.84RR702 pKa = 11.84TINLEE707 pKa = 3.6GSKK710 pKa = 10.85ACIRR714 pKa = 11.84NDD716 pKa = 3.11AYY718 pKa = 11.75SNLIACNVSRR728 pKa = 11.84AKK730 pKa = 10.32CGRR733 pKa = 11.84DD734 pKa = 3.08AAGSDD739 pKa = 3.75SVNSARR745 pKa = 11.84EE746 pKa = 4.1SVPTRR751 pKa = 11.84TPKK754 pKa = 10.18EE755 pKa = 3.72WGGSRR760 pKa = 11.84KK761 pKa = 9.68RR762 pKa = 11.84NPGCAVRR769 pKa = 11.84RR770 pKa = 11.84EE771 pKa = 4.29GVRR774 pKa = 11.84EE775 pKa = 3.78GDD777 pKa = 2.8RR778 pKa = 11.84SEE780 pKa = 4.46FNGTGSHH787 pKa = 5.17RR788 pKa = 11.84TDD790 pKa = 3.39YY791 pKa = 11.04GRR793 pKa = 11.84KK794 pKa = 8.39KK795 pKa = 9.99PSRR798 pKa = 11.84KK799 pKa = 10.01GGFKK803 pKa = 10.02VHH805 pKa = 6.7

Molecular weight: 91.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q80852|Q80852_9BROM Coat protein OS=Parietaria mottle virus OX=64958 PE=2 SV=1
MM1 pKa = 7.45YY2 pKa = 10.39EE3 pKa = 4.52LFNHH7 pKa = 6.35SLQPNQLTPCLRR19 pKa = 11.84TRR21 pKa = 11.84ANGGQVSNRR30 pKa = 11.84QSRR33 pKa = 11.84NVRR36 pKa = 11.84RR37 pKa = 11.84AAAFRR42 pKa = 11.84NSQQTSARR50 pKa = 11.84IPVPVPVIPVSRR62 pKa = 11.84PNGPKK67 pKa = 10.33ASLKK71 pKa = 10.67LPNNQVWVYY80 pKa = 10.62KK81 pKa = 10.37VASEE85 pKa = 4.44LAAKK89 pKa = 9.78TSDD92 pKa = 3.49ANDD95 pKa = 4.76AISLTTMLSGISDD108 pKa = 3.72VKK110 pKa = 11.04PEE112 pKa = 3.98TKK114 pKa = 10.33LYY116 pKa = 10.04RR117 pKa = 11.84LVFGFVAEE125 pKa = 4.28SDD127 pKa = 3.67GSFGVVEE134 pKa = 4.69DD135 pKa = 4.63EE136 pKa = 4.18NVSGNVVPDD145 pKa = 3.81PPVVGRR151 pKa = 11.84AGFKK155 pKa = 10.29KK156 pKa = 8.89HH157 pKa = 5.32TNKK160 pKa = 9.86CRR162 pKa = 11.84DD163 pKa = 3.22VNLEE167 pKa = 3.75GKK169 pKa = 8.16TPDD172 pKa = 3.48EE173 pKa = 4.77LKK175 pKa = 11.15NMAVVWCLDD184 pKa = 3.57EE185 pKa = 4.02NRR187 pKa = 11.84KK188 pKa = 7.52VAKK191 pKa = 10.2RR192 pKa = 11.84IAFTHH197 pKa = 6.0FWFAISRR204 pKa = 11.84PSPLMPPEE212 pKa = 4.78NILVDD217 pKa = 4.26GNQQ220 pKa = 2.93

Molecular weight: 24.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2621

205

1098

524.2

58.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.066 ± 0.855

2.251 ± 0.215

7.02 ± 0.682

5.799 ± 0.185

4.273 ± 0.335

4.922 ± 0.424

2.633 ± 0.428

5.151 ± 0.439

6.219 ± 0.337

9.271 ± 0.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.167

4.388 ± 0.568

5.151 ± 0.918

2.671 ± 0.338

5.799 ± 0.246

8.394 ± 0.294

5.799 ± 0.766

8.127 ± 0.445

1.03 ± 0.078

2.709 ± 0.539
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski