Plantactinospora sp. KBS50
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5179 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A248YWG9|A0A248YWG9_9ACTN Uncharacterized protein OS=Plantactinospora sp. KBS50 OX=2024580 GN=CIK06_15420 PE=4 SV=1
MM1 pKa = 7.13 VGYY4 pKa = 10.26 NVTIMNEE11 pKa = 3.98 SALDD15 pKa = 3.58 GMNAILFQEE24 pKa = 4.66 MPSLPSDD31 pKa = 3.48 AVTLAWMSKK40 pKa = 7.68 MCHH43 pKa = 6.42 PSTSIEE49 pKa = 4.17 YY50 pKa = 9.29 QWSIDD55 pKa = 3.51 YY56 pKa = 11.06 SFVWGQVGTLVPGTQFVAGQILAADD81 pKa = 3.8 LTEE84 pKa = 4.47 NNLVTLSYY92 pKa = 11.24 VDD94 pKa = 3.68 GGFEE98 pKa = 4.17 FGPLGSSGKK107 pKa = 10.31 DD108 pKa = 3.04 GSLTIKK114 pKa = 10.47 EE115 pKa = 4.6 DD116 pKa = 3.57 GSVPGPGNADD126 pKa = 3.23 QGSVGIGMSGAGTFVVPTLTNAGVEE151 pKa = 4.27 FDD153 pKa = 4.52 PKK155 pKa = 8.02 PTYY158 pKa = 9.07 WVAFGSFASSEE169 pKa = 4.11 VMDD172 pKa = 3.93 ISEE175 pKa = 4.82 LTTPAQVTYY184 pKa = 10.59 PDD186 pKa = 4.23 GLTSAKK192 pKa = 10.17 AVYY195 pKa = 9.72 DD196 pKa = 3.76 GKK198 pKa = 10.92 EE199 pKa = 3.46 WTITYY204 pKa = 10.66 GNN206 pKa = 3.72
Molecular weight: 21.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A248YLN4|A0A248YLN4_9ACTN LLM class flavin-dependent oxidoreductase OS=Plantactinospora sp. KBS50 OX=2024580 GN=CIK06_11060 PE=4 SV=1
MM1 pKa = 6.74 VGLRR5 pKa = 11.84 VGHH8 pKa = 6.87 LRR10 pKa = 11.84 LTRR13 pKa = 11.84 PGRR16 pKa = 11.84 GRR18 pKa = 11.84 SAHH21 pKa = 6.65 PGRR24 pKa = 11.84 TVRR27 pKa = 11.84 ATTGLGARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 PVTRR43 pKa = 11.84 PAGAAAIGPRR53 pKa = 11.84 PVVVAAA59 pKa = 4.74
Molecular weight: 6.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5179
0
5179
1768842
30
4222
341.5
36.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.263 ± 0.051
0.791 ± 0.01
6.087 ± 0.03
4.842 ± 0.035
2.62 ± 0.019
9.73 ± 0.031
2.083 ± 0.016
3.227 ± 0.024
1.379 ± 0.021
10.539 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.574 ± 0.013
1.735 ± 0.02
6.595 ± 0.04
2.673 ± 0.017
8.815 ± 0.042
4.756 ± 0.027
5.916 ± 0.032
8.734 ± 0.029
1.551 ± 0.016
2.089 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here