Rosellinia necatrix partitivirus 6
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0YTQ4|A0A0P0YTQ4_9VIRU RNA dependent RNA polymerase OS=Rosellinia necatrix partitivirus 6 OX=1573459 GN=RdRp PE=4 SV=1
MM1 pKa = 7.47 SSVFNNVRR9 pKa = 11.84 NYY11 pKa = 10.01 LQEE14 pKa = 4.1 RR15 pKa = 11.84 LQHH18 pKa = 6.52 VKK20 pKa = 10.59 RR21 pKa = 11.84 EE22 pKa = 4.04 WQIYY26 pKa = 7.4 QATGASSSNMTEE38 pKa = 3.63 EE39 pKa = 5.98 RR40 pKa = 11.84 IQQLQDD46 pKa = 2.93 LDD48 pKa = 3.87 TRR50 pKa = 11.84 RR51 pKa = 11.84 MFHH54 pKa = 5.45 TARR57 pKa = 11.84 NAFDD61 pKa = 3.63 TTTNTPGPVSRR72 pKa = 11.84 ITEE75 pKa = 3.88 EE76 pKa = 3.89 QRR78 pKa = 11.84 RR79 pKa = 11.84 LIYY82 pKa = 10.37 DD83 pKa = 3.76 AEE85 pKa = 4.31 SDD87 pKa = 3.8 KK88 pKa = 11.36 IMNALRR94 pKa = 11.84 QHH96 pKa = 7.1 DD97 pKa = 4.26 EE98 pKa = 3.85 MRR100 pKa = 11.84 SQPFEE105 pKa = 4.42 LFMTRR110 pKa = 11.84 PDD112 pKa = 3.97 SDD114 pKa = 5.07 PIPPNRR120 pKa = 11.84 IAAPGIWQSPLQFHH134 pKa = 6.81 TGQIVHH140 pKa = 7.46 ADD142 pKa = 3.53 PSTTSVLHH150 pKa = 6.44 PDD152 pKa = 2.99 EE153 pKa = 6.67 HH154 pKa = 6.69 EE155 pKa = 5.31 DD156 pKa = 3.69 YY157 pKa = 11.33 AEE159 pKa = 4.43 SYY161 pKa = 11.14 LPGDD165 pKa = 3.29 TDD167 pKa = 2.9 QGYY170 pKa = 10.31 EE171 pKa = 3.98 IDD173 pKa = 3.63 PTIYY177 pKa = 10.52 EE178 pKa = 4.4 LLTRR182 pKa = 11.84 KK183 pKa = 9.82 YY184 pKa = 10.38 PEE186 pKa = 4.1 YY187 pKa = 10.66 LPYY190 pKa = 10.03 AQQYY194 pKa = 8.22 CRR196 pKa = 11.84 PKK198 pKa = 9.73 GTTDD202 pKa = 2.79 GTFRR206 pKa = 11.84 DD207 pKa = 4.32 FNKK210 pKa = 9.87 EE211 pKa = 3.86 QKK213 pKa = 9.74 PCPPLDD219 pKa = 3.57 SDD221 pKa = 4.35 RR222 pKa = 11.84 KK223 pKa = 8.5 EE224 pKa = 4.14 HH225 pKa = 5.66 VLKK228 pKa = 10.95 HH229 pKa = 4.31 VFKK232 pKa = 10.92 RR233 pKa = 11.84 LAIDD237 pKa = 4.35 PYY239 pKa = 11.16 LPLHH243 pKa = 6.44 FVDD246 pKa = 3.82 TQYY249 pKa = 11.64 CKK251 pKa = 10.91 LPLVTGTGYY260 pKa = 10.77 HH261 pKa = 5.45 NRR263 pKa = 11.84 YY264 pKa = 9.68 SYY266 pKa = 10.24 RR267 pKa = 11.84 QKK269 pKa = 10.09 AHH271 pKa = 6.8 ASFSHH276 pKa = 6.42 PLQYY280 pKa = 10.89 GSRR283 pKa = 11.84 PTSKK287 pKa = 10.74 GYY289 pKa = 10.3 FYY291 pKa = 11.19 NATYY295 pKa = 10.76 EE296 pKa = 3.93 NARR299 pKa = 11.84 TIIHH303 pKa = 6.57 NIKK306 pKa = 10.42 EE307 pKa = 4.2 SGVPFNIHH315 pKa = 6.42 FAPEE319 pKa = 4.05 DD320 pKa = 3.66 RR321 pKa = 11.84 DD322 pKa = 3.71 ITDD325 pKa = 3.61 TEE327 pKa = 3.57 IDD329 pKa = 3.54 EE330 pKa = 4.63 YY331 pKa = 11.17 RR332 pKa = 11.84 RR333 pKa = 11.84 KK334 pKa = 10.51 CNNFFDD340 pKa = 4.24 QYY342 pKa = 9.59 PTLLFTRR349 pKa = 11.84 NHH351 pKa = 5.84 ISNRR355 pKa = 11.84 DD356 pKa = 3.1 GTLKK360 pKa = 10.22 VRR362 pKa = 11.84 PVYY365 pKa = 10.59 AVDD368 pKa = 4.8 DD369 pKa = 3.63 IFIIIEE375 pKa = 4.05 AMLTFPALVQARR387 pKa = 11.84 KK388 pKa = 9.12 PDD390 pKa = 3.61 CCIMYY395 pKa = 10.41 GLEE398 pKa = 4.22 TIRR401 pKa = 11.84 GANHH405 pKa = 6.97 KK406 pKa = 9.95 LDD408 pKa = 3.97 SLAQSFSTYY417 pKa = 8.13 FTIDD421 pKa = 2.55 WSGYY425 pKa = 6.69 DD426 pKa = 3.13 QRR428 pKa = 11.84 LPRR431 pKa = 11.84 AITDD435 pKa = 3.85 LFYY438 pKa = 10.74 TDD440 pKa = 3.65 FLRR443 pKa = 11.84 RR444 pKa = 11.84 LIVISHH450 pKa = 7.03 GYY452 pKa = 8.33 QPTYY456 pKa = 10.43 EE457 pKa = 4.22 YY458 pKa = 8.46 PTYY461 pKa = 10.52 PDD463 pKa = 3.97 LNEE466 pKa = 4.66 DD467 pKa = 3.38 NMYY470 pKa = 11.51 DD471 pKa = 5.17 RR472 pKa = 11.84 MDD474 pKa = 4.61 KK475 pKa = 10.51 LLKK478 pKa = 9.37 WLHH481 pKa = 5.72 LWYY484 pKa = 11.22 NNMTFVTADD493 pKa = 3.54 GYY495 pKa = 11.44 AYY497 pKa = 10.18 RR498 pKa = 11.84 RR499 pKa = 11.84 MYY501 pKa = 10.98 AGVPSGLFNTQFLDD515 pKa = 3.59 SFANLYY521 pKa = 10.27 ILIDD525 pKa = 3.59 GMIEE529 pKa = 3.91 FGFTDD534 pKa = 3.65 EE535 pKa = 5.32 EE536 pKa = 4.23 IDD538 pKa = 3.86 SFLLFVLGDD547 pKa = 4.22 DD548 pKa = 3.99 NSGMTNLSLARR559 pKa = 11.84 LHH561 pKa = 6.65 EE562 pKa = 4.98 FIQFLEE568 pKa = 4.76 AYY570 pKa = 9.66 ALTRR574 pKa = 11.84 YY575 pKa = 10.27 HH576 pKa = 6.22 MVLSHH581 pKa = 6.38 TKK583 pKa = 10.35 SVITALRR590 pKa = 11.84 NKK592 pKa = 9.77 IEE594 pKa = 4.13 SLGYY598 pKa = 9.25 QCNFGLPKK606 pKa = 9.82 RR607 pKa = 11.84 DD608 pKa = 2.97 IGKK611 pKa = 9.63 LVAQLIYY618 pKa = 9.87 PEE620 pKa = 4.9 HH621 pKa = 6.8 KK622 pKa = 9.87 IKK624 pKa = 10.68 YY625 pKa = 7.91 HH626 pKa = 4.87 TMSARR631 pKa = 11.84 AIGLAYY637 pKa = 10.23 ASCAYY642 pKa = 10.57 DD643 pKa = 3.03 KK644 pKa = 11.03 TFYY647 pKa = 10.81 NFCKK651 pKa = 10.22 SIYY654 pKa = 10.37 NIFLDD659 pKa = 3.92 YY660 pKa = 11.37 YY661 pKa = 10.63 EE662 pKa = 4.84 YY663 pKa = 10.89 DD664 pKa = 3.42 EE665 pKa = 4.52 KK666 pKa = 10.76 TALNLSRR673 pKa = 11.84 FLTTGQDD680 pKa = 3.46 DD681 pKa = 4.31 LTTQFSWKK689 pKa = 9.53 ILPPFPTRR697 pKa = 11.84 EE698 pKa = 4.19 EE699 pKa = 3.55 IRR701 pKa = 11.84 KK702 pKa = 9.61 QISFYY707 pKa = 10.8 HH708 pKa = 6.42 GPLDD712 pKa = 4.94 YY713 pKa = 10.97 APKK716 pKa = 9.86 WNFAHH721 pKa = 7.39 FINKK725 pKa = 8.99 PDD727 pKa = 4.0 VIPPSSKK734 pKa = 9.47 TMYY737 pKa = 10.16 DD738 pKa = 3.47 YY739 pKa = 10.95 EE740 pKa = 4.68 IEE742 pKa = 4.3 HH743 pKa = 6.55 SLRR746 pKa = 11.84 PQPAPTFVARR756 pKa = 4.36
Molecular weight: 88.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.754
IPC2_protein 5.766
IPC_protein 5.855
Toseland 6.326
ProMoST 6.237
Dawson 6.148
Bjellqvist 6.148
Wikipedia 6.173
Rodwell 6.135
Grimsley 6.517
Solomon 6.148
Lehninger 6.148
Nozaki 6.427
DTASelect 6.62
Thurlkill 6.634
EMBOSS 6.605
Sillero 6.532
Patrickios 3.63
IPC_peptide 6.16
IPC2_peptide 6.517
IPC2.peptide.svr19 6.43
Protein with the highest isoelectric point:
>tr|A0A0P0YTQ4|A0A0P0YTQ4_9VIRU RNA dependent RNA polymerase OS=Rosellinia necatrix partitivirus 6 OX=1573459 GN=RdRp PE=4 SV=1
MM1 pKa = 7.5 SSKK4 pKa = 11.08 LNLSDD9 pKa = 3.5 RR10 pKa = 11.84 AKK12 pKa = 10.46 KK13 pKa = 10.41 VSALKK18 pKa = 10.13 TEE20 pKa = 4.37 TTFKK24 pKa = 10.92 AIDD27 pKa = 3.98 LPGPTANFTTAFKK40 pKa = 10.59 KK41 pKa = 10.86 GSAPKK46 pKa = 9.38 TGLANEE52 pKa = 4.53 HH53 pKa = 5.86 YY54 pKa = 10.72 VYY56 pKa = 11.08 VLPDD60 pKa = 3.79 FSNILMFIIYY70 pKa = 9.47 HH71 pKa = 7.15 AIQFGPSLEE80 pKa = 4.37 IKK82 pKa = 9.82 NHH84 pKa = 5.93 PKK86 pKa = 9.97 VSIPVFVSYY95 pKa = 11.07 CLTLVYY101 pKa = 11.04 AHH103 pKa = 6.64 FLVSDD108 pKa = 4.13 AYY110 pKa = 9.83 INPGQPHH117 pKa = 6.4 FAMTIKK123 pKa = 10.61 NDD125 pKa = 3.63 SAYY128 pKa = 10.84 SEE130 pKa = 4.33 YY131 pKa = 11.21 LDD133 pKa = 4.62 FLLTLPVPEE142 pKa = 4.65 FLTPLFAKK150 pKa = 8.2 FTSTTTSRR158 pKa = 11.84 RR159 pKa = 11.84 TNTWFLASAEE169 pKa = 4.08 GFMHH173 pKa = 6.2 FTHH176 pKa = 7.45 FGRR179 pKa = 11.84 FFPINLFLNMHH190 pKa = 6.91 DD191 pKa = 3.4 LAARR195 pKa = 11.84 TEE197 pKa = 4.33 SRR199 pKa = 11.84 ANPAEE204 pKa = 3.98 VFYY207 pKa = 10.58 DD208 pKa = 3.74 LNMTEE213 pKa = 4.46 IFHH216 pKa = 5.28 ITKK219 pKa = 10.15 YY220 pKa = 10.14 RR221 pKa = 11.84 DD222 pKa = 3.25 PSVTTDD228 pKa = 2.98 TAVTYY233 pKa = 10.05 RR234 pKa = 11.84 YY235 pKa = 10.12 HH236 pKa = 7.13 NFFASLYY243 pKa = 6.6 PTTATTKK250 pKa = 8.87 TRR252 pKa = 11.84 RR253 pKa = 11.84 SMNFFYY259 pKa = 10.44 EE260 pKa = 4.05 SRR262 pKa = 11.84 INQVLEE268 pKa = 3.98 GLFNPVLLRR277 pKa = 11.84 AQQQRR282 pKa = 11.84 QAFSRR287 pKa = 11.84 ISIEE291 pKa = 3.84 APTFEE296 pKa = 4.18 TANYY300 pKa = 9.5 NPYY303 pKa = 10.06 FALLAITKK311 pKa = 9.54 EE312 pKa = 4.15 NIPEE316 pKa = 4.38 LMTVMQSVADD326 pKa = 4.65 TINGSIPIKK335 pKa = 10.27 SQLNKK340 pKa = 10.1 MYY342 pKa = 10.56 DD343 pKa = 3.3 NYY345 pKa = 11.25 SGVSILDD352 pKa = 3.06 HH353 pKa = 6.86 GYY355 pKa = 11.23 AGFPTPTWDD364 pKa = 4.3 ADD366 pKa = 4.02 SNLPDD371 pKa = 4.77 NDD373 pKa = 3.46 TTLDD377 pKa = 3.37 KK378 pKa = 10.74 FVYY381 pKa = 10.26 NNAVLKK387 pKa = 10.85 FEE389 pKa = 5.37 GIPLSNYY396 pKa = 9.28 AAAIKK401 pKa = 10.25 FKK403 pKa = 10.89 GAISSQSWTLSNNIIARR420 pKa = 11.84 IDD422 pKa = 3.32 RR423 pKa = 11.84 SEE425 pKa = 4.15 AVPNVTDD432 pKa = 4.15 PAPDD436 pKa = 3.6 TVIVTTTITPGSAYY450 pKa = 10.68 LSLLALATVTDD461 pKa = 3.7 RR462 pKa = 11.84 HH463 pKa = 6.2 DD464 pKa = 3.61 VASSSTFYY472 pKa = 11.14 LPNTASGPTDD482 pKa = 3.72 EE483 pKa = 5.82 NDD485 pKa = 3.62 HH486 pKa = 5.35 QHH488 pKa = 5.41 MTTGNHH494 pKa = 5.19 VLRR497 pKa = 11.84 SYY499 pKa = 10.82 SAEE502 pKa = 3.74 LDD504 pKa = 3.29 NYY506 pKa = 8.39 PTVKK510 pKa = 10.22 VLQFAEE516 pKa = 4.35 TDD518 pKa = 3.74 DD519 pKa = 3.68 EE520 pKa = 4.43 TAYY523 pKa = 10.78 QATLFGTVIYY533 pKa = 10.49 SDD535 pKa = 4.09 EE536 pKa = 4.22 LVGSIVNHH544 pKa = 5.69 PHH546 pKa = 5.1 PTSRR550 pKa = 11.84 VEE552 pKa = 3.98 EE553 pKa = 4.48 DD554 pKa = 2.82 NSLFLTSCIQFRR566 pKa = 11.84 TTHH569 pKa = 6.79 LATNFKK575 pKa = 9.45 TNNPIASIIMKK586 pKa = 9.17 TPAPTNDD593 pKa = 3.08 AQFALSLYY601 pKa = 9.74 RR602 pKa = 11.84 SPNVTIPRR610 pKa = 11.84 ITTHH614 pKa = 5.81 TAHH617 pKa = 6.5 SGYY620 pKa = 10.5 HH621 pKa = 6.1 RR622 pKa = 11.84 DD623 pKa = 3.05 TYY625 pKa = 11.12 GLHH628 pKa = 7.13 RR629 pKa = 11.84 MTTNISVPEE638 pKa = 4.13 FSQSILSYY646 pKa = 7.73 TVRR649 pKa = 11.84 DD650 pKa = 3.73 RR651 pKa = 11.84 PSRR654 pKa = 11.84 RR655 pKa = 11.84 HH656 pKa = 4.81 QAPIHH661 pKa = 5.97 EE662 pKa = 5.09 PPSTPYY668 pKa = 10.61 YY669 pKa = 10.57 RR670 pKa = 11.84 LLLWSPYY677 pKa = 10.6 VFVTPNYY684 pKa = 9.9 NRR686 pKa = 11.84 DD687 pKa = 3.11 WEE689 pKa = 4.1 QDD691 pKa = 3.21 EE692 pKa = 4.78 FGQALLDD699 pKa = 3.45 HH700 pKa = 7.03 HH701 pKa = 7.11 YY702 pKa = 9.33 VTNLRR707 pKa = 11.84 TIFGLDD713 pKa = 2.69 ATMIEE718 pKa = 4.4 VRR720 pKa = 11.84 SGSAAMPVAA729 pKa = 4.54
Molecular weight: 81.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.893
IPC2_protein 5.817
IPC_protein 5.919
Toseland 6.504
ProMoST 6.326
Dawson 6.237
Bjellqvist 6.224
Wikipedia 6.262
Rodwell 6.224
Grimsley 6.737
Solomon 6.237
Lehninger 6.237
Nozaki 6.517
DTASelect 6.722
Thurlkill 6.751
EMBOSS 6.707
Sillero 6.62
Patrickios 3.795
IPC_peptide 6.249
IPC2_peptide 6.605
IPC2.peptide.svr19 6.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1485
729
756
742.5
85.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.936 ± 0.89
0.741 ± 0.321
6.128 ± 0.724
4.579 ± 0.413
5.993 ± 0.123
3.704 ± 0.283
3.569 ± 0.002
6.061 ± 0.206
3.838 ± 0.376
8.485 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.155 ± 0.067
5.32 ± 0.587
6.465 ± 0.082
3.502 ± 0.616
5.387 ± 0.686
6.936 ± 0.89
9.091 ± 1.2
4.31 ± 0.809
0.808 ± 0.084
5.993 ± 0.725
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here