Paenibacillus phage LincolnB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gochnauervirinae; Wanderervirus; unclassified Wanderervirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ATX7|A0A345ATX7_9CAUD Uncharacterized protein OS=Paenibacillus phage LincolnB OX=2249769 GN=LINCOLNB_60 PE=4 SV=1
MM1 pKa = 7.85IYY3 pKa = 10.56GIIDD7 pKa = 3.64GSVLIEE13 pKa = 4.16TYY15 pKa = 7.4EE16 pKa = 4.31TPEE19 pKa = 3.84EE20 pKa = 4.13AYY22 pKa = 9.85EE23 pKa = 4.07AAKK26 pKa = 10.24VAYY29 pKa = 7.61EE30 pKa = 3.82ATGDD34 pKa = 3.76FFGVIAVANDD44 pKa = 3.33AKK46 pKa = 11.02GIADD50 pKa = 3.64NAA52 pKa = 4.12

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ATU2|A0A345ATU2_9CAUD Uncharacterized protein OS=Paenibacillus phage LincolnB OX=2249769 GN=LINCOLNB_25 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84KK3 pKa = 8.52EE4 pKa = 3.81LKK6 pKa = 10.21RR7 pKa = 11.84FIRR10 pKa = 11.84NGVSEE15 pKa = 4.08TLRR18 pKa = 11.84YY19 pKa = 9.33EE20 pKa = 3.88KK21 pKa = 10.92AFDD24 pKa = 2.94VWRR27 pKa = 11.84IKK29 pKa = 10.56RR30 pKa = 11.84LRR32 pKa = 11.84DD33 pKa = 3.33LLFVSS38 pKa = 4.85

Molecular weight: 4.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12744

38

1233

177.0

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.552 ± 0.509

1.083 ± 0.154

6.113 ± 0.311

8.271 ± 0.396

3.97 ± 0.216

6.199 ± 0.28

1.766 ± 0.17

6.733 ± 0.334

8.467 ± 0.277

8.992 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.137

4.7 ± 0.201

3.241 ± 0.167

3.994 ± 0.177

5.265 ± 0.374

5.304 ± 0.257

5.1 ± 0.199

6.536 ± 0.28

1.334 ± 0.151

3.602 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski