Amino acid dipepetide frequency for Paenibacillus phage LincolnB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.473AlaAla: 4.473 ± 0.847
0.549AlaCys: 0.549 ± 0.201
4.238AlaAsp: 4.238 ± 0.58
6.121AlaGlu: 6.121 ± 0.666
3.061AlaPhe: 3.061 ± 0.603
4.473AlaGly: 4.473 ± 0.735
0.863AlaHis: 0.863 ± 0.242
4.316AlaIle: 4.316 ± 0.589
5.493AlaLys: 5.493 ± 0.672
6.278AlaLeu: 6.278 ± 0.922
2.276AlaMet: 2.276 ± 0.442
1.883AlaAsn: 1.883 ± 0.398
1.883AlaPro: 1.883 ± 0.419
2.747AlaGln: 2.747 ± 0.463
2.982AlaArg: 2.982 ± 0.414
3.531AlaSer: 3.531 ± 0.657
2.668AlaThr: 2.668 ± 0.505
4.395AlaVal: 4.395 ± 0.992
0.942AlaTrp: 0.942 ± 0.254
2.354AlaTyr: 2.354 ± 0.5
0.0AlaXaa: 0.0 ± 0.0
Cys
0.471CysAla: 0.471 ± 0.169
0.314CysCys: 0.314 ± 0.2
0.628CysAsp: 0.628 ± 0.249
0.549CysGlu: 0.549 ± 0.194
0.549CysPhe: 0.549 ± 0.226
0.942CysGly: 0.942 ± 0.274
0.235CysHis: 0.235 ± 0.144
0.785CysIle: 0.785 ± 0.204
1.099CysLys: 1.099 ± 0.308
0.706CysLeu: 0.706 ± 0.275
0.235CysMet: 0.235 ± 0.122
0.471CysAsn: 0.471 ± 0.204
0.549CysPro: 0.549 ± 0.204
0.314CysGln: 0.314 ± 0.185
0.785CysArg: 0.785 ± 0.277
0.549CysSer: 0.549 ± 0.231
0.0CysThr: 0.0 ± 0.0
0.785CysVal: 0.785 ± 0.268
0.314CysTrp: 0.314 ± 0.16
0.549CysTyr: 0.549 ± 0.193
0.0CysXaa: 0.0 ± 0.0
Asp
4.316AspAla: 4.316 ± 0.548
0.314AspCys: 0.314 ± 0.142
3.845AspAsp: 3.845 ± 0.539
4.787AspGlu: 4.787 ± 0.592
2.825AspPhe: 2.825 ± 0.449
4.787AspGly: 4.787 ± 0.558
0.706AspHis: 0.706 ± 0.266
4.316AspIle: 4.316 ± 0.512
4.002AspLys: 4.002 ± 0.681
4.787AspLeu: 4.787 ± 0.526
2.433AspMet: 2.433 ± 0.449
2.825AspAsn: 2.825 ± 0.425
2.119AspPro: 2.119 ± 0.552
2.354AspGln: 2.354 ± 0.447
3.374AspArg: 3.374 ± 0.568
1.805AspSer: 1.805 ± 0.358
3.374AspThr: 3.374 ± 0.612
5.101AspVal: 5.101 ± 0.686
1.177AspTrp: 1.177 ± 0.318
1.883AspTyr: 1.883 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
5.493GluAla: 5.493 ± 0.674
0.392GluCys: 0.392 ± 0.205
5.022GluAsp: 5.022 ± 0.617
6.827GluGlu: 6.827 ± 1.04
3.61GluPhe: 3.61 ± 0.454
3.767GluGly: 3.767 ± 0.472
1.726GluHis: 1.726 ± 0.444
5.729GluIle: 5.729 ± 0.627
6.827GluLys: 6.827 ± 0.894
8.083GluLeu: 8.083 ± 0.797
1.962GluMet: 1.962 ± 0.409
3.688GluAsn: 3.688 ± 0.596
1.569GluPro: 1.569 ± 0.392
5.65GluGln: 5.65 ± 0.788
4.395GluArg: 4.395 ± 0.509
4.238GluSer: 4.238 ± 0.626
4.238GluThr: 4.238 ± 0.517
5.729GluVal: 5.729 ± 0.818
1.02GluTrp: 1.02 ± 0.468
2.747GluTyr: 2.747 ± 0.5
0.0GluXaa: 0.0 ± 0.0
Phe
2.276PheAla: 2.276 ± 0.635
0.471PheCys: 0.471 ± 0.187
3.139PheAsp: 3.139 ± 0.446
3.531PheGlu: 3.531 ± 0.617
1.256PhePhe: 1.256 ± 0.354
2.982PheGly: 2.982 ± 0.4
0.628PheHis: 0.628 ± 0.293
2.982PheIle: 2.982 ± 0.462
3.61PheLys: 3.61 ± 0.475
3.296PheLeu: 3.296 ± 0.497
0.785PheMet: 0.785 ± 0.283
1.962PheAsn: 1.962 ± 0.428
1.413PhePro: 1.413 ± 0.317
1.962PheGln: 1.962 ± 0.454
1.569PheArg: 1.569 ± 0.339
2.511PheSer: 2.511 ± 0.514
1.491PheThr: 1.491 ± 0.436
1.962PheVal: 1.962 ± 0.378
0.471PheTrp: 0.471 ± 0.195
1.413PheTyr: 1.413 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
3.531GlyAla: 3.531 ± 0.739
0.471GlyCys: 0.471 ± 0.197
4.708GlyAsp: 4.708 ± 0.506
5.493GlyGlu: 5.493 ± 0.684
2.433GlyPhe: 2.433 ± 0.282
5.022GlyGly: 5.022 ± 0.779
1.413GlyHis: 1.413 ± 0.332
4.552GlyIle: 4.552 ± 0.754
5.65GlyLys: 5.65 ± 0.688
5.415GlyLeu: 5.415 ± 0.677
1.569GlyMet: 1.569 ± 0.369
3.061GlyAsn: 3.061 ± 0.677
0.471GlyPro: 0.471 ± 0.163
1.726GlyGln: 1.726 ± 0.375
2.511GlyArg: 2.511 ± 0.482
2.511GlySer: 2.511 ± 0.403
2.982GlyThr: 2.982 ± 0.467
4.944GlyVal: 4.944 ± 0.638
1.02GlyTrp: 1.02 ± 0.35
2.511GlyTyr: 2.511 ± 0.459
0.0GlyXaa: 0.0 ± 0.0
His
1.256HisAla: 1.256 ± 0.33
0.235HisCys: 0.235 ± 0.12
1.177HisAsp: 1.177 ± 0.301
1.805HisGlu: 1.805 ± 0.393
0.942HisPhe: 0.942 ± 0.29
1.02HisGly: 1.02 ± 0.269
0.157HisHis: 0.157 ± 0.108
1.099HisIle: 1.099 ± 0.336
0.942HisLys: 0.942 ± 0.302
2.354HisLeu: 2.354 ± 0.503
0.314HisMet: 0.314 ± 0.15
0.706HisAsn: 0.706 ± 0.208
0.628HisPro: 0.628 ± 0.243
1.177HisGln: 1.177 ± 0.277
1.02HisArg: 1.02 ± 0.277
0.706HisSer: 0.706 ± 0.239
0.628HisThr: 0.628 ± 0.24
0.863HisVal: 0.863 ± 0.219
0.157HisTrp: 0.157 ± 0.107
0.471HisTyr: 0.471 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
4.787IleAla: 4.787 ± 0.95
1.099IleCys: 1.099 ± 0.267
4.002IleAsp: 4.002 ± 0.505
6.121IleGlu: 6.121 ± 0.739
2.119IlePhe: 2.119 ± 0.384
4.944IleGly: 4.944 ± 0.698
0.942IleHis: 0.942 ± 0.246
4.081IleIle: 4.081 ± 0.726
6.592IleLys: 6.592 ± 0.81
5.336IleLeu: 5.336 ± 0.544
1.177IleMet: 1.177 ± 0.396
2.668IleAsn: 2.668 ± 0.411
3.061IlePro: 3.061 ± 0.563
2.982IleGln: 2.982 ± 0.456
3.217IleArg: 3.217 ± 0.48
3.688IleSer: 3.688 ± 0.484
3.453IleThr: 3.453 ± 0.508
4.865IleVal: 4.865 ± 0.6
0.785IleTrp: 0.785 ± 0.221
1.413IleTyr: 1.413 ± 0.313
0.0IleXaa: 0.0 ± 0.0
Lys
5.65LysAla: 5.65 ± 0.749
0.549LysCys: 0.549 ± 0.215
4.316LysAsp: 4.316 ± 0.581
6.906LysGlu: 6.906 ± 0.7
3.374LysPhe: 3.374 ± 0.476
4.944LysGly: 4.944 ± 0.524
1.883LysHis: 1.883 ± 0.426
5.101LysIle: 5.101 ± 0.645
6.513LysLys: 6.513 ± 0.892
8.083LysLeu: 8.083 ± 0.705
2.511LysMet: 2.511 ± 0.397
4.002LysAsn: 4.002 ± 0.457
2.668LysPro: 2.668 ± 0.464
3.061LysGln: 3.061 ± 0.425
5.493LysArg: 5.493 ± 0.803
4.708LysSer: 4.708 ± 0.567
5.336LysThr: 5.336 ± 0.649
4.63LysVal: 4.63 ± 0.542
1.569LysTrp: 1.569 ± 0.358
3.374LysTyr: 3.374 ± 0.621
0.0LysXaa: 0.0 ± 0.0
Leu
6.749LeuAla: 6.749 ± 0.724
1.177LeuCys: 1.177 ± 0.344
5.101LeuAsp: 5.101 ± 0.625
8.004LeuGlu: 8.004 ± 0.78
3.374LeuPhe: 3.374 ± 0.466
5.336LeuGly: 5.336 ± 0.857
1.962LeuHis: 1.962 ± 0.391
4.865LeuIle: 4.865 ± 0.512
6.827LeuLys: 6.827 ± 0.683
6.278LeuLeu: 6.278 ± 0.623
2.119LeuMet: 2.119 ± 0.356
5.493LeuAsn: 5.493 ± 0.612
3.688LeuPro: 3.688 ± 0.597
3.845LeuGln: 3.845 ± 0.584
5.101LeuArg: 5.101 ± 0.708
6.435LeuSer: 6.435 ± 0.538
5.964LeuThr: 5.964 ± 0.566
4.002LeuVal: 4.002 ± 0.446
1.177LeuTrp: 1.177 ± 0.503
2.433LeuTyr: 2.433 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
1.334MetAla: 1.334 ± 0.333
0.314MetCys: 0.314 ± 0.149
0.785MetAsp: 0.785 ± 0.193
2.354MetGlu: 2.354 ± 0.318
0.863MetPhe: 0.863 ± 0.265
2.04MetGly: 2.04 ± 0.328
0.549MetHis: 0.549 ± 0.237
1.883MetIle: 1.883 ± 0.396
2.747MetLys: 2.747 ± 0.529
2.747MetLeu: 2.747 ± 0.436
0.471MetMet: 0.471 ± 0.186
2.04MetAsn: 2.04 ± 0.507
1.177MetPro: 1.177 ± 0.297
0.942MetGln: 0.942 ± 0.316
1.491MetArg: 1.491 ± 0.334
2.197MetSer: 2.197 ± 0.425
1.334MetThr: 1.334 ± 0.41
1.648MetVal: 1.648 ± 0.375
0.078MetTrp: 0.078 ± 0.062
0.785MetTyr: 0.785 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
4.238AsnAla: 4.238 ± 0.682
0.628AsnCys: 0.628 ± 0.216
2.747AsnAsp: 2.747 ± 0.411
2.668AsnGlu: 2.668 ± 0.485
1.491AsnPhe: 1.491 ± 0.304
3.845AsnGly: 3.845 ± 0.655
0.471AsnHis: 0.471 ± 0.152
2.904AsnIle: 2.904 ± 0.444
3.531AsnLys: 3.531 ± 0.461
4.395AsnLeu: 4.395 ± 0.508
1.177AsnMet: 1.177 ± 0.34
2.04AsnAsn: 2.04 ± 0.463
1.962AsnPro: 1.962 ± 0.4
1.569AsnGln: 1.569 ± 0.37
3.061AsnArg: 3.061 ± 0.549
2.825AsnSer: 2.825 ± 0.486
2.276AsnThr: 2.276 ± 0.355
3.061AsnVal: 3.061 ± 0.437
0.392AsnTrp: 0.392 ± 0.169
1.726AsnTyr: 1.726 ± 0.366
0.0AsnXaa: 0.0 ± 0.0
Pro
1.648ProAla: 1.648 ± 0.378
0.471ProCys: 0.471 ± 0.187
1.648ProAsp: 1.648 ± 0.367
2.904ProGlu: 2.904 ± 0.414
1.491ProPhe: 1.491 ± 0.289
1.413ProGly: 1.413 ± 0.342
0.942ProHis: 0.942 ± 0.218
2.59ProIle: 2.59 ± 0.452
3.139ProLys: 3.139 ± 0.705
3.139ProLeu: 3.139 ± 0.47
0.942ProMet: 0.942 ± 0.335
1.648ProAsn: 1.648 ± 0.298
1.177ProPro: 1.177 ± 0.344
0.942ProGln: 0.942 ± 0.286
1.334ProArg: 1.334 ± 0.302
2.04ProSer: 2.04 ± 0.553
1.491ProThr: 1.491 ± 0.298
2.433ProVal: 2.433 ± 0.616
0.157ProTrp: 0.157 ± 0.12
0.863ProTyr: 0.863 ± 0.231
0.0ProXaa: 0.0 ± 0.0
Gln
3.139GlnAla: 3.139 ± 0.597
0.157GlnCys: 0.157 ± 0.128
2.04GlnAsp: 2.04 ± 0.496
3.217GlnGlu: 3.217 ± 0.499
1.805GlnPhe: 1.805 ± 0.353
1.726GlnGly: 1.726 ± 0.369
1.02GlnHis: 1.02 ± 0.311
2.511GlnIle: 2.511 ± 0.423
2.747GlnLys: 2.747 ± 0.444
4.708GlnLeu: 4.708 ± 0.629
1.962GlnMet: 1.962 ± 0.369
1.569GlnAsn: 1.569 ± 0.328
1.02GlnPro: 1.02 ± 0.283
1.413GlnGln: 1.413 ± 0.354
2.04GlnArg: 2.04 ± 0.491
1.726GlnSer: 1.726 ± 0.343
1.962GlnThr: 1.962 ± 0.269
2.982GlnVal: 2.982 ± 0.378
0.392GlnTrp: 0.392 ± 0.198
1.805GlnTyr: 1.805 ± 0.508
0.0GlnXaa: 0.0 ± 0.0
Arg
2.825ArgAla: 2.825 ± 0.421
0.314ArgCys: 0.314 ± 0.153
2.668ArgAsp: 2.668 ± 0.461
4.159ArgGlu: 4.159 ± 0.69
1.805ArgPhe: 1.805 ± 0.421
2.354ArgGly: 2.354 ± 0.47
1.413ArgHis: 1.413 ± 0.351
4.159ArgIle: 4.159 ± 0.689
5.179ArgLys: 5.179 ± 0.812
4.865ArgLeu: 4.865 ± 0.663
1.491ArgMet: 1.491 ± 0.339
2.747ArgAsn: 2.747 ± 0.408
1.569ArgPro: 1.569 ± 0.387
1.883ArgGln: 1.883 ± 0.365
3.296ArgArg: 3.296 ± 0.656
2.197ArgSer: 2.197 ± 0.438
2.982ArgThr: 2.982 ± 0.469
3.296ArgVal: 3.296 ± 0.597
1.099ArgTrp: 1.099 ± 0.305
2.354ArgTyr: 2.354 ± 0.54
0.0ArgXaa: 0.0 ± 0.0
Ser
3.924SerAla: 3.924 ± 0.553
0.706SerCys: 0.706 ± 0.232
2.04SerAsp: 2.04 ± 0.389
4.159SerGlu: 4.159 ± 0.644
2.59SerPhe: 2.59 ± 0.433
3.688SerGly: 3.688 ± 0.445
0.942SerHis: 0.942 ± 0.335
4.473SerIle: 4.473 ± 0.669
4.787SerLys: 4.787 ± 0.601
4.081SerLeu: 4.081 ± 0.673
1.962SerMet: 1.962 ± 0.315
2.59SerAsn: 2.59 ± 0.499
1.805SerPro: 1.805 ± 0.351
1.334SerGln: 1.334 ± 0.32
3.688SerArg: 3.688 ± 0.629
2.59SerSer: 2.59 ± 0.501
2.747SerThr: 2.747 ± 0.423
2.511SerVal: 2.511 ± 0.433
0.392SerTrp: 0.392 ± 0.182
2.04SerTyr: 2.04 ± 0.433
0.0SerXaa: 0.0 ± 0.0
Thr
2.825ThrAla: 2.825 ± 0.578
0.628ThrCys: 0.628 ± 0.199
2.982ThrAsp: 2.982 ± 0.502
3.688ThrGlu: 3.688 ± 0.518
1.726ThrPhe: 1.726 ± 0.26
3.217ThrGly: 3.217 ± 0.561
0.471ThrHis: 0.471 ± 0.231
3.453ThrIle: 3.453 ± 0.432
5.022ThrLys: 5.022 ± 0.578
5.022ThrLeu: 5.022 ± 0.729
1.256ThrMet: 1.256 ± 0.33
2.197ThrAsn: 2.197 ± 0.452
2.04ThrPro: 2.04 ± 0.402
1.491ThrGln: 1.491 ± 0.34
2.276ThrArg: 2.276 ± 0.417
2.433ThrSer: 2.433 ± 0.38
3.217ThrThr: 3.217 ± 0.556
3.767ThrVal: 3.767 ± 0.495
0.785ThrTrp: 0.785 ± 0.254
2.511ThrTyr: 2.511 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
4.159ValAla: 4.159 ± 0.563
0.706ValCys: 0.706 ± 0.317
5.964ValAsp: 5.964 ± 0.612
5.258ValGlu: 5.258 ± 0.721
2.982ValPhe: 2.982 ± 0.529
2.825ValGly: 2.825 ± 0.368
0.471ValHis: 0.471 ± 0.174
4.63ValIle: 4.63 ± 1.207
5.493ValLys: 5.493 ± 0.634
5.729ValLeu: 5.729 ± 0.653
1.569ValMet: 1.569 ± 0.383
3.296ValAsn: 3.296 ± 0.484
2.276ValPro: 2.276 ± 0.354
2.197ValGln: 2.197 ± 0.324
2.354ValArg: 2.354 ± 0.355
4.159ValSer: 4.159 ± 0.617
3.374ValThr: 3.374 ± 0.639
4.708ValVal: 4.708 ± 0.667
0.863ValTrp: 0.863 ± 0.264
2.276ValTyr: 2.276 ± 0.507
0.0ValXaa: 0.0 ± 0.0
Trp
0.785TrpAla: 0.785 ± 0.209
0.471TrpCys: 0.471 ± 0.174
1.099TrpAsp: 1.099 ± 0.328
0.706TrpGlu: 0.706 ± 0.199
0.314TrpPhe: 0.314 ± 0.139
0.863TrpGly: 0.863 ± 0.327
0.235TrpHis: 0.235 ± 0.147
0.549TrpIle: 0.549 ± 0.23
1.177TrpLys: 1.177 ± 0.297
1.569TrpLeu: 1.569 ± 0.318
0.235TrpMet: 0.235 ± 0.107
0.628TrpAsn: 0.628 ± 0.281
0.078TrpPro: 0.078 ± 0.081
0.392TrpGln: 0.392 ± 0.224
1.02TrpArg: 1.02 ± 0.328
0.785TrpSer: 0.785 ± 0.325
0.235TrpThr: 0.235 ± 0.145
0.942TrpVal: 0.942 ± 0.28
0.314TrpTrp: 0.314 ± 0.154
0.942TrpTyr: 0.942 ± 0.27
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.648TyrAla: 1.648 ± 0.361
0.863TyrCys: 0.863 ± 0.264
2.982TyrAsp: 2.982 ± 0.563
3.453TyrGlu: 3.453 ± 0.594
1.099TyrPhe: 1.099 ± 0.326
1.805TyrGly: 1.805 ± 0.411
0.628TyrHis: 0.628 ± 0.225
2.354TyrIle: 2.354 ± 0.481
3.296TyrLys: 3.296 ± 0.487
3.061TyrLeu: 3.061 ± 0.59
1.256TyrMet: 1.256 ± 0.336
1.491TyrAsn: 1.491 ± 0.365
1.256TyrPro: 1.256 ± 0.262
1.962TyrGln: 1.962 ± 0.399
1.648TyrArg: 1.648 ± 0.377
1.413TyrSer: 1.413 ± 0.333
1.256TyrThr: 1.256 ± 0.321
2.747TyrVal: 2.747 ± 0.507
0.235TyrTrp: 0.235 ± 0.134
1.569TyrTyr: 1.569 ± 0.404
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (12744 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski