Monosporascus sp. mg162

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4W395|A0A4Q4W395_9PEZI AAA domain-containing protein OS=Monosporascus sp. mg162 OX=1081914 GN=DL768_010686 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84LLVLSTCLLAAGNASPFAPDD23 pKa = 3.46IKK25 pKa = 10.79PRR27 pKa = 11.84DD28 pKa = 3.55ICDD31 pKa = 3.33GNTATTRR38 pKa = 11.84TEE40 pKa = 3.42WCDD43 pKa = 3.17FDD45 pKa = 6.49INTDD49 pKa = 3.63YY50 pKa = 11.5YY51 pKa = 10.37NTVPEE56 pKa = 4.23TGVTRR61 pKa = 11.84EE62 pKa = 4.14YY63 pKa = 10.77FLEE66 pKa = 4.4LQDD69 pKa = 3.71VTVSPDD75 pKa = 3.25GVSRR79 pKa = 11.84PAIAVNGSIPGPTIFADD96 pKa = 3.05WGDD99 pKa = 3.8TVVVHH104 pKa = 5.46VTNSLTTSGNGSSIHH119 pKa = 5.28FHH121 pKa = 7.33GIRR124 pKa = 11.84QNFTNQNDD132 pKa = 4.22GVVSITQCPTAVGDD146 pKa = 3.67TTTYY150 pKa = 7.76TWRR153 pKa = 11.84ATQYY157 pKa = 10.72GSTWYY162 pKa = 9.69HH163 pKa = 4.67SHH165 pKa = 7.29FALQAWEE172 pKa = 4.54GVFGGIIINGPASSNYY188 pKa = 10.4DD189 pKa = 3.28EE190 pKa = 5.03DD191 pKa = 4.78LGMVFLNDD199 pKa = 3.43WSHH202 pKa = 4.29QTVDD206 pKa = 3.89EE207 pKa = 5.1LYY209 pKa = 7.78MTAQTSGPPTLDD221 pKa = 2.86NGLINGTNVFGSDD234 pKa = 4.83DD235 pKa = 4.24SDD237 pKa = 3.85DD238 pKa = 3.79QTGSRR243 pKa = 11.84LEE245 pKa = 4.01LSVTEE250 pKa = 4.13GTSYY254 pKa = 11.26RR255 pKa = 11.84LRR257 pKa = 11.84LVNAAIDD264 pKa = 3.54THH266 pKa = 6.65FKK268 pKa = 10.84FSVDD272 pKa = 2.97NHH274 pKa = 5.46TLTVIGMDD282 pKa = 3.86LVPIEE287 pKa = 4.83PYY289 pKa = 7.57TTEE292 pKa = 3.91FVSIGIADD300 pKa = 3.98QATVATDD307 pKa = 3.28FWMRR311 pKa = 11.84AIPQVACSDD320 pKa = 3.55NDD322 pKa = 3.68SADD325 pKa = 3.62NIRR328 pKa = 11.84AIVHH332 pKa = 6.09YY333 pKa = 9.88GDD335 pKa = 3.69STATPSTTAFEE346 pKa = 4.49YY347 pKa = 10.64TDD349 pKa = 3.4SCDD352 pKa = 5.87DD353 pKa = 3.8EE354 pKa = 6.78DD355 pKa = 4.79SSNLVPIVSKK365 pKa = 11.06AVGDD369 pKa = 3.86GSNDD373 pKa = 2.85ILEE376 pKa = 4.42IATVGTVDD384 pKa = 5.58NLFKK388 pKa = 10.61WFLNSTTMVVDD399 pKa = 3.56WADD402 pKa = 3.42PTLLQLSNNITTFDD416 pKa = 3.91DD417 pKa = 3.41SDD419 pKa = 4.05AVLEE423 pKa = 4.46LPNVDD428 pKa = 2.55EE429 pKa = 4.09WVYY432 pKa = 11.26VVIQTTFAVPHH443 pKa = 7.1PIHH446 pKa = 6.48LHH448 pKa = 5.3GFDD451 pKa = 4.51FFVLAHH457 pKa = 5.45GTGTFSSDD465 pKa = 3.06SVTLNTDD472 pKa = 3.01NPPRR476 pKa = 11.84RR477 pKa = 11.84DD478 pKa = 3.33TAMLPASGYY487 pKa = 10.47LVLAFEE493 pKa = 4.81TDD495 pKa = 3.92NPGAWLMHH503 pKa = 5.82CHH505 pKa = 6.47IGWHH509 pKa = 4.85TSEE512 pKa = 4.88GFALQFVVRR521 pKa = 11.84RR522 pKa = 11.84DD523 pKa = 3.8EE524 pKa = 4.26ILDD527 pKa = 4.78LIDD530 pKa = 3.42TTTLEE535 pKa = 4.58DD536 pKa = 3.42TCSAWSSYY544 pKa = 10.14AAEE547 pKa = 4.52GSIEE551 pKa = 3.99QDD553 pKa = 3.09DD554 pKa = 4.23SGVV557 pKa = 3.11

Molecular weight: 60.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4V0N7|A0A4Q4V0N7_9PEZI Uncharacterized protein OS=Monosporascus sp. mg162 OX=1081914 GN=DL768_011671 PE=4 SV=1
MM1 pKa = 7.88PEE3 pKa = 3.79GAAPAATAHH12 pKa = 6.37GGVEE16 pKa = 4.71AYY18 pKa = 10.12AVGTPGEE25 pKa = 4.62RR26 pKa = 11.84GTQAAQARR34 pKa = 11.84LGTARR39 pKa = 11.84ALRR42 pKa = 11.84GTLARR47 pKa = 11.84GMRR50 pKa = 11.84GGRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84GSRR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84LGRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84GSNSSLGGGSSGLGGSGPGALGGQALGASGPGANGSSTLSRR117 pKa = 11.84GRR119 pKa = 11.84HH120 pKa = 4.89LPP122 pKa = 3.46

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11877

0

11877

5863050

66

6420

493.6

54.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.227 ± 0.02

1.176 ± 0.008

5.922 ± 0.015

6.391 ± 0.024

3.527 ± 0.012

7.278 ± 0.02

2.344 ± 0.008

4.532 ± 0.014

4.632 ± 0.021

8.782 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.007

3.527 ± 0.012

6.157 ± 0.024

3.867 ± 0.014

6.583 ± 0.023

7.827 ± 0.025

5.764 ± 0.017

6.199 ± 0.017

1.467 ± 0.007

2.717 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski