Streptococcus satellite phage Javan324
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJT5|A0A4D5ZJT5_9VIRU Transcriptional regulator OS=Streptococcus satellite phage Javan324 OX=2558637 GN=JavanS324_0002 PE=4 SV=1
MM1 pKa = 6.83 FTIEE5 pKa = 4.22 EE6 pKa = 5.06 LINADD11 pKa = 4.55 KK12 pKa = 11.19 EE13 pKa = 4.48 NNTNFADD20 pKa = 3.41 ILINYY25 pKa = 9.02 ISLNDD30 pKa = 3.75 YY31 pKa = 10.3 DD32 pKa = 4.95 KK33 pKa = 11.34 KK34 pKa = 10.82 IAFDD38 pKa = 4.31 LVQKK42 pKa = 11.0 LSDD45 pKa = 3.4 RR46 pKa = 11.84 DD47 pKa = 3.72 KK48 pKa = 11.36 EE49 pKa = 4.22 KK50 pKa = 10.9 EE51 pKa = 3.85
Molecular weight: 6.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.556
IPC2_protein 4.406
IPC_protein 4.266
Toseland 4.075
ProMoST 4.317
Dawson 4.228
Bjellqvist 4.469
Wikipedia 4.151
Rodwell 4.088
Grimsley 3.986
Solomon 4.228
Lehninger 4.177
Nozaki 4.355
DTASelect 4.546
Thurlkill 4.113
EMBOSS 4.164
Sillero 4.38
Patrickios 4.139
IPC_peptide 4.228
IPC2_peptide 4.355
IPC2.peptide.svr19 4.326
Protein with the highest isoelectric point:
>tr|A0A4D5ZJQ4|A0A4D5ZJQ4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan324 OX=2558637 GN=JavanS324_0005 PE=4 SV=1
MM1 pKa = 8.02 LITQEE6 pKa = 3.8 QAKK9 pKa = 10.18 ALRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.98 KK15 pKa = 10.76 ADD17 pKa = 3.54 LQLKK21 pKa = 9.78 NYY23 pKa = 9.36 EE24 pKa = 4.03 LALEE28 pKa = 4.32 IGVASRR34 pKa = 11.84 TVPKK38 pKa = 10.16 ILKK41 pKa = 9.88 GDD43 pKa = 3.5 YY44 pKa = 9.18 KK45 pKa = 10.76 APKK48 pKa = 9.3 RR49 pKa = 11.84 IYY51 pKa = 10.12 ASVMEE56 pKa = 4.73 WLAKK60 pKa = 10.37 DD61 pKa = 3.6 YY62 pKa = 11.54
Molecular weight: 7.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.023
IPC2_protein 9.268
IPC_protein 9.209
Toseland 9.97
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.794
Wikipedia 10.306
Rodwell 10.701
Grimsley 10.218
Solomon 10.189
Lehninger 10.16
Nozaki 9.926
DTASelect 9.794
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.058
Patrickios 10.423
IPC_peptide 10.189
IPC2_peptide 8.244
IPC2.peptide.svr19 8.229
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
3022
51
388
159.1
18.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.732 ± 0.441
0.629 ± 0.083
6.42 ± 0.483
7.842 ± 0.613
4.335 ± 0.331
4.533 ± 0.484
1.555 ± 0.307
6.916 ± 0.347
11.118 ± 0.503
9.96 ± 0.393
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.886 ± 0.281
6.122 ± 0.59
2.184 ± 0.313
4.07 ± 0.267
4.5 ± 0.411
6.386 ± 0.58
6.717 ± 0.568
4.798 ± 0.414
0.761 ± 0.109
4.533 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here