Beet cryptic virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Deltapartitivirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3W850|E3W850_9VIRU RNA-dependent RNA polymerase OS=Beet cryptic virus 2 OX=912029 PE=4 SV=1
MM1 pKa = 7.53TGPTQAHH8 pKa = 7.23LIEE11 pKa = 4.71ATSFTGLNRR20 pKa = 11.84DD21 pKa = 3.7EE22 pKa = 5.26LDD24 pKa = 4.04SIVDD28 pKa = 3.68ALQEE32 pKa = 4.12EE33 pKa = 4.67NQIGTFRR40 pKa = 11.84DD41 pKa = 3.27RR42 pKa = 11.84PKK44 pKa = 9.92AYY46 pKa = 9.45RR47 pKa = 11.84RR48 pKa = 11.84YY49 pKa = 8.54VHH51 pKa = 6.4VRR53 pKa = 11.84SQEE56 pKa = 3.83LFDD59 pKa = 4.91RR60 pKa = 11.84LSEE63 pKa = 4.24LYY65 pKa = 10.84ADD67 pKa = 5.01LFTSNWSAFKK77 pKa = 10.1HH78 pKa = 6.07AYY80 pKa = 6.98ITEE83 pKa = 3.98PTGRR87 pKa = 11.84EE88 pKa = 3.86DD89 pKa = 4.13ANSISTKK96 pKa = 10.26ASFMAQCYY104 pKa = 6.41LTAWFWDD111 pKa = 3.48TQYY114 pKa = 11.12CIRR117 pKa = 11.84EE118 pKa = 4.35SVRR121 pKa = 11.84KK122 pKa = 9.92LSGTAYY128 pKa = 9.16NQHH131 pKa = 5.33FTEE134 pKa = 4.78DD135 pKa = 3.93LNLISPKK142 pKa = 9.95YY143 pKa = 10.52DD144 pKa = 3.2PFLQHH149 pKa = 6.93LNMVIRR155 pKa = 11.84PTLIHH160 pKa = 6.64QSTEE164 pKa = 3.64DD165 pKa = 3.22TLYY168 pKa = 10.54IPLLGQTFNYY178 pKa = 10.62NEE180 pKa = 4.03DD181 pKa = 3.43AYY183 pKa = 11.71NFLNLQGCGTEE194 pKa = 3.64IRR196 pKa = 11.84QVYY199 pKa = 10.35AITDD203 pKa = 3.41VMDD206 pKa = 3.4SRR208 pKa = 11.84RR209 pKa = 11.84TEE211 pKa = 3.46WSTIPLATNVLGRR224 pKa = 11.84ASWLLDD230 pKa = 3.65FKK232 pKa = 11.15QGNAYY237 pKa = 10.21AWFPFEE243 pKa = 5.71SNFTEE248 pKa = 4.41LDD250 pKa = 3.27LVAPYY255 pKa = 10.41ILGIPCTPRR264 pKa = 11.84LGPRR268 pKa = 11.84DD269 pKa = 3.67EE270 pKa = 5.48DD271 pKa = 4.72HH272 pKa = 6.11YY273 pKa = 10.19QHH275 pKa = 6.47WTHH278 pKa = 6.72NDD280 pKa = 3.4PPLSTDD286 pKa = 3.2GNIILNPLAYY296 pKa = 10.13VRR298 pKa = 11.84SSEE301 pKa = 4.31RR302 pKa = 11.84KK303 pKa = 8.02FFGNAEE309 pKa = 4.17YY310 pKa = 8.53RR311 pKa = 11.84TMEE314 pKa = 4.21YY315 pKa = 11.01NSYY318 pKa = 8.15TQNFEE323 pKa = 4.38AYY325 pKa = 8.22MPRR328 pKa = 11.84VTTSTQAKK336 pKa = 9.41NKK338 pKa = 8.92RR339 pKa = 11.84KK340 pKa = 9.73KK341 pKa = 10.31DD342 pKa = 3.46AAPEE346 pKa = 3.88QLSTGQGDD354 pKa = 4.13GSSSSKK360 pKa = 10.26DD361 pKa = 3.11AEE363 pKa = 4.21MSTEE367 pKa = 3.99EE368 pKa = 4.67SEE370 pKa = 5.01APPPPKK376 pKa = 9.64IPRR379 pKa = 11.84PRR381 pKa = 11.84QIHH384 pKa = 4.63QFRR387 pKa = 11.84LIDD390 pKa = 3.46WVYY393 pKa = 9.58HH394 pKa = 4.98SRR396 pKa = 11.84VILGADD402 pKa = 2.92KK403 pKa = 11.12AMQRR407 pKa = 11.84RR408 pKa = 11.84ALWDD412 pKa = 5.01FIHH415 pKa = 7.3AGATPSHH422 pKa = 6.01QPRR425 pKa = 11.84PP426 pKa = 3.79

Molecular weight: 49.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3W851|E3W851_9VIRU Viral protein 2 OS=Beet cryptic virus 2 OX=912029 PE=4 SV=1
MM1 pKa = 7.33RR2 pKa = 11.84TINNYY7 pKa = 9.83EE8 pKa = 3.99YY9 pKa = 10.9TSFTEE14 pKa = 4.84DD15 pKa = 4.77LEE17 pKa = 4.55EE18 pKa = 3.71TDD20 pKa = 3.54YY21 pKa = 10.75THH23 pKa = 6.4PHH25 pKa = 4.67VVRR28 pKa = 11.84RR29 pKa = 11.84DD30 pKa = 3.6PEE32 pKa = 4.05VTYY35 pKa = 11.11EE36 pKa = 3.77DD37 pKa = 3.57TFAKK41 pKa = 10.44KK42 pKa = 9.92EE43 pKa = 3.89LLSRR47 pKa = 11.84YY48 pKa = 7.41PALYY52 pKa = 10.28EE53 pKa = 3.58NLIRR57 pKa = 11.84GWSRR61 pKa = 11.84SYY63 pKa = 9.97YY64 pKa = 9.74TGQEE68 pKa = 3.81HH69 pKa = 6.8LRR71 pKa = 11.84AIMQYY76 pKa = 8.8ATPNTNFSEE85 pKa = 4.83CVQHH89 pKa = 7.26AYY91 pKa = 9.26TKK93 pKa = 10.59AITKK97 pKa = 7.89VTEE100 pKa = 4.32SLHH103 pKa = 5.91SLPTVRR109 pKa = 11.84AFNVLEE115 pKa = 4.11EE116 pKa = 4.79LDD118 pKa = 4.67LIPYY122 pKa = 8.58EE123 pKa = 4.47SSSSAGYY130 pKa = 8.08TYY132 pKa = 10.64RR133 pKa = 11.84GVKK136 pKa = 10.04GPQHH140 pKa = 6.7GEE142 pKa = 3.33NHH144 pKa = 4.87MQAIKK149 pKa = 8.88TAKK152 pKa = 9.27AVLWSVIKK160 pKa = 10.6DD161 pKa = 3.37DD162 pKa = 4.33GEE164 pKa = 4.73GIEE167 pKa = 4.88HH168 pKa = 6.96VIDD171 pKa = 3.81TYY173 pKa = 11.66VPDD176 pKa = 3.23VGYY179 pKa = 8.69TRR181 pKa = 11.84TQLTDD186 pKa = 3.16LRR188 pKa = 11.84EE189 pKa = 4.0KK190 pKa = 10.37MKK192 pKa = 10.7VRR194 pKa = 11.84GVWGRR199 pKa = 11.84AFHH202 pKa = 6.67YY203 pKa = 10.23ILLEE207 pKa = 4.28GTSAAPLLEE216 pKa = 4.51AFANSNTFFHH226 pKa = 6.81IGKK229 pKa = 9.34DD230 pKa = 3.42PTVSVPYY237 pKa = 10.16LISYY241 pKa = 7.69TKK243 pKa = 10.66GQAPWLTAIDD253 pKa = 3.76WQAFDD258 pKa = 3.57ATVSRR263 pKa = 11.84FEE265 pKa = 4.11INAAFDD271 pKa = 3.98IIKK274 pKa = 10.76SKK276 pKa = 9.16ITFPNLEE283 pKa = 4.02TEE285 pKa = 4.16QAFEE289 pKa = 3.98ISRR292 pKa = 11.84QLFIHH297 pKa = 6.64KK298 pKa = 10.0KK299 pKa = 9.23LAAPNGKK306 pKa = 9.01IYY308 pKa = 10.24RR309 pKa = 11.84IHH311 pKa = 7.46KK312 pKa = 8.12GIPSGSYY319 pKa = 7.09FTSIIGSVVNRR330 pKa = 11.84LRR332 pKa = 11.84IEE334 pKa = 4.11YY335 pKa = 9.9LWNLKK340 pKa = 9.39FNRR343 pKa = 11.84GPKK346 pKa = 9.46VCFTQGDD353 pKa = 3.81DD354 pKa = 3.99SLIGDD359 pKa = 4.56DD360 pKa = 4.38EE361 pKa = 5.56LYY363 pKa = 11.24SPMDD367 pKa = 3.01MAAFVKK373 pKa = 9.81PLNWFINTSKK383 pKa = 11.28SMTSKK388 pKa = 10.3VPEE391 pKa = 5.02AITFLGRR398 pKa = 11.84SSLGGLNQRR407 pKa = 11.84DD408 pKa = 4.01LKK410 pKa = 10.89RR411 pKa = 11.84CLRR414 pKa = 11.84LLILPEE420 pKa = 4.1YY421 pKa = 9.34PVTSGDD427 pKa = 2.92ISAFRR432 pKa = 11.84ANSIWRR438 pKa = 11.84DD439 pKa = 3.48SGSTSQILHH448 pKa = 6.92EE449 pKa = 4.51IANALRR455 pKa = 11.84RR456 pKa = 11.84KK457 pKa = 10.01YY458 pKa = 10.68GIAKK462 pKa = 8.65EE463 pKa = 4.04QDD465 pKa = 3.13VPRR468 pKa = 11.84YY469 pKa = 9.12LRR471 pKa = 11.84PWKK474 pKa = 10.55AA475 pKa = 2.95

Molecular weight: 54.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1294

393

475

431.3

49.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.651 ± 0.315

1.005 ± 0.227

5.641 ± 0.382

5.719 ± 0.452

4.328 ± 0.196

4.714 ± 0.496

2.782 ± 0.108

6.414 ± 0.483

4.328 ± 0.647

8.346 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.777 ± 0.152

5.255 ± 0.678

5.641 ± 0.386

3.941 ± 0.531

6.646 ± 0.273

6.569 ± 0.785

8.269 ± 0.587

4.096 ± 0.566

2.009 ± 0.056

4.869 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski