Agathobaculum butyriciproducens
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2545 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1BFX9|A0A2U1BFX9_9FIRM Uncharacterized protein OS=Agathobaculum butyriciproducens OX=1628085 GN=C7386_102158 PE=4 SV=1
MM1 pKa = 7.69 EE2 pKa = 4.72 EE3 pKa = 3.69 MRR5 pKa = 11.84 VYY7 pKa = 10.25 IANLGKK13 pKa = 10.45 YY14 pKa = 9.93 NEE16 pKa = 4.62 GEE18 pKa = 3.95 LVGAWFEE25 pKa = 4.02 PPIDD29 pKa = 3.75 FDD31 pKa = 4.02 EE32 pKa = 4.56 VKK34 pKa = 10.65 EE35 pKa = 4.16 RR36 pKa = 11.84 IGLNDD41 pKa = 3.13 QYY43 pKa = 11.4 EE44 pKa = 4.64 EE45 pKa = 4.17 YY46 pKa = 10.37 AIHH49 pKa = 7.48 DD50 pKa = 3.8 FEE52 pKa = 5.92 LPFEE56 pKa = 4.4 IEE58 pKa = 4.13 EE59 pKa = 4.01 YY60 pKa = 10.75 TPIEE64 pKa = 4.52 EE65 pKa = 4.21 INRR68 pKa = 11.84 LCEE71 pKa = 3.99 MVEE74 pKa = 4.25 DD75 pKa = 4.12 LPDD78 pKa = 3.88 YY79 pKa = 10.97 IRR81 pKa = 11.84 DD82 pKa = 3.71 NMGSLLSYY90 pKa = 10.6 YY91 pKa = 10.67 SGVEE95 pKa = 4.12 DD96 pKa = 4.1 FCEE99 pKa = 4.01 HH100 pKa = 7.14 ADD102 pKa = 6.15 DD103 pKa = 5.28 IILWSGCDD111 pKa = 3.15 TTADD115 pKa = 3.43 VAEE118 pKa = 4.06 QMLDD122 pKa = 2.98 EE123 pKa = 4.79 GLISDD128 pKa = 3.87 EE129 pKa = 3.88 TLRR132 pKa = 11.84 RR133 pKa = 11.84 YY134 pKa = 9.7 FDD136 pKa = 3.38 SVQYY140 pKa = 11.03 GRR142 pKa = 11.84 DD143 pKa = 3.15 LSMEE147 pKa = 4.11 GDD149 pKa = 3.76 FVEE152 pKa = 4.68 TKK154 pKa = 10.74 DD155 pKa = 4.43 GVFEE159 pKa = 5.36 LIRR162 pKa = 5.25
Molecular weight: 18.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.605
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.63
Grimsley 3.516
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.91
Patrickios 1.125
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A2U1BDR0|A0A2U1BDR0_9FIRM Ribonuclease H OS=Agathobaculum butyriciproducens OX=1628085 GN=rnhA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.85 KK9 pKa = 7.62 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.69 EE15 pKa = 3.5 HH16 pKa = 6.14 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.23 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.49 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2545
0
2545
787198
27
3344
309.3
34.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.542 ± 0.054
1.632 ± 0.02
5.854 ± 0.041
6.63 ± 0.053
3.817 ± 0.036
7.427 ± 0.052
1.986 ± 0.028
6.071 ± 0.045
5.442 ± 0.042
9.252 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.901 ± 0.025
3.741 ± 0.039
3.818 ± 0.028
3.491 ± 0.033
5.332 ± 0.055
5.612 ± 0.041
5.993 ± 0.061
7.031 ± 0.047
0.903 ± 0.015
3.522 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here