Candidatus Kinetoplastibacterium sorsogonicusi
Average proteome isoelectric point is 7.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 670 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S7J9Q3|A0A3S7J9Q3_9PROT Miniconductance mechanosensitive channel YbdG OS=Candidatus Kinetoplastibacterium sorsogonicusi OX=1576550 GN=ybdG PE=4 SV=1
MM1 pKa = 7.17 QFKK4 pKa = 10.41 DD5 pKa = 3.36 QEE7 pKa = 4.43 LLVDD11 pKa = 4.11 VSTAAIDD18 pKa = 3.37 TGGYY22 pKa = 10.42 GVFLTVTAEE31 pKa = 4.0 GGTEE35 pKa = 3.42 IGTTFSYY42 pKa = 10.65 LGSCEE47 pKa = 4.02 SLYY50 pKa = 10.11 EE51 pKa = 4.0 ARR53 pKa = 11.84 YY54 pKa = 8.64 LAEE57 pKa = 4.34 NFAKK61 pKa = 10.54 NWVAEE66 pKa = 4.11 NLLSS70 pKa = 4.03
Molecular weight: 7.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.861
IPC2_protein 4.088
IPC_protein 3.872
Toseland 3.732
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.732
Grimsley 3.656
Solomon 3.808
Lehninger 3.757
Nozaki 3.986
DTASelect 4.05
Thurlkill 3.77
EMBOSS 3.757
Sillero 3.986
Patrickios 0.604
IPC_peptide 3.808
IPC2_peptide 3.973
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A3Q8EQY5|A0A3Q8EQY5_9PROT Transport permease protein OS=Candidatus Kinetoplastibacterium sorsogonicusi OX=1576550 GN=yadH PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.17 RR14 pKa = 11.84 THH16 pKa = 5.95 GFRR19 pKa = 11.84 IRR21 pKa = 11.84 MKK23 pKa = 9.35 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.56 GRR39 pKa = 11.84 KK40 pKa = 8.68 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
670
0
670
222456
30
1851
332.0
37.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.954 ± 0.094
1.153 ± 0.028
5.113 ± 0.064
5.337 ± 0.075
4.659 ± 0.071
5.535 ± 0.084
1.963 ± 0.029
12.489 ± 0.122
8.673 ± 0.088
9.787 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.17 ± 0.041
8.416 ± 0.115
3.024 ± 0.046
2.804 ± 0.042
3.359 ± 0.068
7.138 ± 0.06
4.165 ± 0.049
4.6 ± 0.074
0.85 ± 0.026
3.811 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here