Legionella sp. km772

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella; unclassified Legionella

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A433K2U4|A0A433K2U4_9GAMM Zinc-finger domain-containing protein OS=Legionella sp. km772 OX=2498111 GN=ELY15_04840 PE=4 SV=1
MM1 pKa = 7.42AAIDD5 pKa = 3.88VSPNTSTDD13 pKa = 2.69IQFSVNAANKK23 pKa = 8.6VAEE26 pKa = 4.67LIKK29 pKa = 10.83EE30 pKa = 4.14EE31 pKa = 4.43EE32 pKa = 4.12NPKK35 pKa = 10.66LNLRR39 pKa = 11.84VSITGGGCSGFQYY52 pKa = 10.64GFSFDD57 pKa = 4.01EE58 pKa = 5.43EE59 pKa = 4.32INEE62 pKa = 4.76DD63 pKa = 3.67DD64 pKa = 4.67TIVEE68 pKa = 4.32QQCSNGEE75 pKa = 4.11NPVKK79 pKa = 10.65LLIDD83 pKa = 3.68SMSYY87 pKa = 10.47QYY89 pKa = 11.48LHH91 pKa = 6.42NAEE94 pKa = 3.76IDD96 pKa = 4.02YY97 pKa = 10.77IQGIQGEE104 pKa = 4.48QFVIRR109 pKa = 11.84NPNAKK114 pKa = 6.55TTCGCGSSFSMEE126 pKa = 5.16DD127 pKa = 3.51DD128 pKa = 4.29DD129 pKa = 7.36DD130 pKa = 4.28YY131 pKa = 12.15LL132 pKa = 5.97

Molecular weight: 14.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S0Y985|A0A3S0Y985_9GAMM S-(hydroxymethyl)glutathione dehydrogenase OS=Legionella sp. km772 OX=2498111 GN=ELY15_04560 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.18RR12 pKa = 11.84KK13 pKa = 8.79RR14 pKa = 11.84DD15 pKa = 3.31HH16 pKa = 6.72GFRR19 pKa = 11.84EE20 pKa = 4.28RR21 pKa = 11.84MATRR25 pKa = 11.84AGRR28 pKa = 11.84LVIKK32 pKa = 10.41RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3061

0

3061

969520

22

4801

316.7

35.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.086 ± 0.049

1.167 ± 0.017

4.651 ± 0.037

6.022 ± 0.049

4.368 ± 0.037

5.933 ± 0.041

2.493 ± 0.024

7.132 ± 0.039

6.262 ± 0.042

11.411 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.019

4.889 ± 0.035

4.107 ± 0.027

4.72 ± 0.033

4.099 ± 0.033

6.733 ± 0.04

5.232 ± 0.032

5.779 ± 0.047

1.111 ± 0.017

3.516 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski