Streptomyces phage Euratis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vashvirus; unclassified Vashvirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411B127|A0A411B127_9CAUD Integrase OS=Streptomyces phage Euratis OX=2510569 GN=57 PE=4 SV=1
MM1 pKa = 7.08ITRR4 pKa = 11.84DD5 pKa = 3.44GARR8 pKa = 11.84GRR10 pKa = 11.84LLPWTTEE17 pKa = 3.66GRR19 pKa = 11.84PCFLEE24 pKa = 5.27ADD26 pKa = 3.98GGVLSQLADD35 pKa = 3.77DD36 pKa = 4.99MEE38 pKa = 4.73DD39 pKa = 3.19AQLAMGQHH47 pKa = 6.42EE48 pKa = 4.84LTNARR53 pKa = 11.84KK54 pKa = 9.65VLADD58 pKa = 3.54PTSPNATVRR67 pKa = 11.84YY68 pKa = 8.71AAIRR72 pKa = 11.84LAEE75 pKa = 4.47CLTDD79 pKa = 3.65ALRR82 pKa = 11.84VAEE85 pKa = 4.24SRR87 pKa = 11.84GMRR90 pKa = 11.84LPDD93 pKa = 4.51PDD95 pKa = 5.31DD96 pKa = 6.07DD97 pKa = 4.71EE98 pKa = 7.0DD99 pKa = 4.58EE100 pKa = 5.27NDD102 pKa = 3.52AQTPAEE108 pKa = 4.03ALGG111 pKa = 3.63

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411B108|A0A411B108_9CAUD Uncharacterized protein OS=Streptomyces phage Euratis OX=2510569 GN=26 PE=4 SV=1
MM1 pKa = 7.37IRR3 pKa = 11.84VTTEE7 pKa = 3.73TKK9 pKa = 10.85AEE11 pKa = 4.01LQTEE15 pKa = 4.62RR16 pKa = 11.84GRR18 pKa = 11.84VVGFVKK24 pKa = 10.45SSEE27 pKa = 3.99PGKK30 pKa = 10.61ISIAVPADD38 pKa = 3.75RR39 pKa = 11.84AVMTPAQARR48 pKa = 11.84QLAAWLNEE56 pKa = 3.65EE57 pKa = 4.14ADD59 pKa = 3.47RR60 pKa = 11.84GGRR63 pKa = 11.84LTAADD68 pKa = 4.03RR69 pKa = 11.84TDD71 pKa = 3.53EE72 pKa = 4.0GWRR75 pKa = 11.84RR76 pKa = 11.84AEE78 pKa = 3.75QEE80 pKa = 3.6RR81 pKa = 11.84RR82 pKa = 11.84DD83 pKa = 4.25AIRR86 pKa = 11.84TALNADD92 pKa = 3.3RR93 pKa = 11.84VTFAGRR99 pKa = 11.84TYY101 pKa = 9.68TRR103 pKa = 11.84RR104 pKa = 11.84GG105 pKa = 3.18

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12365

34

811

213.2

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.414 ± 0.423

0.841 ± 0.133

6.818 ± 0.222

5.879 ± 0.373

3.195 ± 0.21

8.459 ± 0.354

1.747 ± 0.165

3.575 ± 0.247

4.408 ± 0.29

7.998 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.147

2.944 ± 0.183

5.305 ± 0.375

2.79 ± 0.161

6.874 ± 0.505

5.507 ± 0.25

6.907 ± 0.385

7.82 ± 0.233

1.868 ± 0.143

2.362 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski