Amino acid dipepetide frequency for Streptomyces phage Euratis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.5AlaAla: 16.5 ± 1.372
1.051AlaCys: 1.051 ± 0.31
9.544AlaAsp: 9.544 ± 1.068
8.331AlaGlu: 8.331 ± 1.055
2.265AlaPhe: 2.265 ± 0.44
8.412AlaGly: 8.412 ± 0.904
2.184AlaHis: 2.184 ± 0.481
5.095AlaIle: 5.095 ± 0.69
5.338AlaLys: 5.338 ± 0.795
11.808AlaLeu: 11.808 ± 1.045
3.64AlaMet: 3.64 ± 0.539
3.316AlaAsn: 3.316 ± 0.528
5.095AlaPro: 5.095 ± 0.777
5.338AlaGln: 5.338 ± 0.719
7.36AlaArg: 7.36 ± 0.934
5.742AlaSer: 5.742 ± 0.872
8.331AlaThr: 8.331 ± 0.823
9.301AlaVal: 9.301 ± 0.75
2.265AlaTrp: 2.265 ± 0.424
3.235AlaTyr: 3.235 ± 0.441
0.0AlaXaa: 0.0 ± 0.0
Cys
1.375CysAla: 1.375 ± 0.403
0.0CysCys: 0.0 ± 0.0
0.647CysAsp: 0.647 ± 0.221
0.566CysGlu: 0.566 ± 0.174
0.243CysPhe: 0.243 ± 0.145
0.728CysGly: 0.728 ± 0.292
0.081CysHis: 0.081 ± 0.082
0.404CysIle: 0.404 ± 0.198
0.243CysLys: 0.243 ± 0.141
0.566CysLeu: 0.566 ± 0.222
0.081CysMet: 0.081 ± 0.086
0.404CysAsn: 0.404 ± 0.149
0.647CysPro: 0.647 ± 0.269
0.243CysGln: 0.243 ± 0.128
0.485CysArg: 0.485 ± 0.172
0.566CysSer: 0.566 ± 0.243
0.162CysThr: 0.162 ± 0.108
0.647CysVal: 0.647 ± 0.242
0.162CysTrp: 0.162 ± 0.109
0.162CysTyr: 0.162 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
9.625AspAla: 9.625 ± 1.077
0.081AspCys: 0.081 ± 0.083
5.581AspAsp: 5.581 ± 0.834
4.61AspGlu: 4.61 ± 0.548
2.507AspPhe: 2.507 ± 0.419
6.875AspGly: 6.875 ± 0.869
1.294AspHis: 1.294 ± 0.379
1.375AspIle: 1.375 ± 0.392
2.346AspLys: 2.346 ± 0.434
6.066AspLeu: 6.066 ± 0.676
1.698AspMet: 1.698 ± 0.375
1.618AspAsn: 1.618 ± 0.279
3.963AspPro: 3.963 ± 0.567
0.89AspGln: 0.89 ± 0.243
4.287AspArg: 4.287 ± 0.767
3.154AspSer: 3.154 ± 0.524
3.397AspThr: 3.397 ± 0.43
6.794AspVal: 6.794 ± 0.768
1.213AspTrp: 1.213 ± 0.301
0.809AspTyr: 0.809 ± 0.245
0.0AspXaa: 0.0 ± 0.0
Glu
7.845GluAla: 7.845 ± 0.877
0.404GluCys: 0.404 ± 0.186
3.154GluAsp: 3.154 ± 0.489
2.912GluGlu: 2.912 ± 0.533
2.75GluPhe: 2.75 ± 0.464
4.125GluGly: 4.125 ± 0.733
1.051GluHis: 1.051 ± 0.31
2.265GluIle: 2.265 ± 0.503
1.86GluLys: 1.86 ± 0.407
6.47GluLeu: 6.47 ± 0.999
1.132GluMet: 1.132 ± 0.28
1.213GluAsn: 1.213 ± 0.359
3.478GluPro: 3.478 ± 1.008
1.375GluGln: 1.375 ± 0.311
4.691GluArg: 4.691 ± 0.622
3.882GluSer: 3.882 ± 0.516
3.478GluThr: 3.478 ± 0.625
3.316GluVal: 3.316 ± 0.522
1.779GluTrp: 1.779 ± 0.369
1.618GluTyr: 1.618 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
4.368PheAla: 4.368 ± 0.631
0.243PheCys: 0.243 ± 0.144
2.669PheAsp: 2.669 ± 0.475
0.89PheGlu: 0.89 ± 0.245
0.566PhePhe: 0.566 ± 0.235
2.75PheGly: 2.75 ± 0.493
0.404PheHis: 0.404 ± 0.195
0.89PheIle: 0.89 ± 0.273
1.375PheLys: 1.375 ± 0.317
2.75PheLeu: 2.75 ± 0.473
0.647PheMet: 0.647 ± 0.261
1.375PheAsn: 1.375 ± 0.35
2.346PhePro: 2.346 ± 0.395
1.051PheGln: 1.051 ± 0.275
2.265PheArg: 2.265 ± 0.424
1.456PheSer: 1.456 ± 0.366
2.993PheThr: 2.993 ± 0.545
1.941PheVal: 1.941 ± 0.314
0.243PheTrp: 0.243 ± 0.133
0.647PheTyr: 0.647 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
9.463GlyAla: 9.463 ± 1.091
1.051GlyCys: 1.051 ± 0.4
5.257GlyAsp: 5.257 ± 0.65
4.61GlyGlu: 4.61 ± 0.62
3.154GlyPhe: 3.154 ± 0.533
6.632GlyGly: 6.632 ± 0.816
0.971GlyHis: 0.971 ± 0.258
3.64GlyIle: 3.64 ± 0.648
4.448GlyLys: 4.448 ± 0.662
7.037GlyLeu: 7.037 ± 1.109
2.346GlyMet: 2.346 ± 0.423
2.588GlyAsn: 2.588 ± 0.398
4.044GlyPro: 4.044 ± 0.61
2.588GlyGln: 2.588 ± 0.383
5.5GlyArg: 5.5 ± 0.607
5.581GlySer: 5.581 ± 0.665
4.691GlyThr: 4.691 ± 0.871
6.228GlyVal: 6.228 ± 0.787
1.86GlyTrp: 1.86 ± 0.387
2.912GlyTyr: 2.912 ± 0.662
0.0GlyXaa: 0.0 ± 0.0
His
2.103HisAla: 2.103 ± 0.423
0.162HisCys: 0.162 ± 0.117
1.213HisAsp: 1.213 ± 0.311
1.456HisGlu: 1.456 ± 0.494
0.566HisPhe: 0.566 ± 0.199
1.051HisGly: 1.051 ± 0.318
0.162HisHis: 0.162 ± 0.12
0.971HisIle: 0.971 ± 0.295
0.404HisLys: 0.404 ± 0.165
1.698HisLeu: 1.698 ± 0.401
0.647HisMet: 0.647 ± 0.267
0.243HisAsn: 0.243 ± 0.131
1.132HisPro: 1.132 ± 0.332
0.243HisGln: 0.243 ± 0.193
1.132HisArg: 1.132 ± 0.249
1.051HisSer: 1.051 ± 0.257
0.971HisThr: 0.971 ± 0.242
1.132HisVal: 1.132 ± 0.33
0.89HisTrp: 0.89 ± 0.267
0.243HisTyr: 0.243 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
4.287IleAla: 4.287 ± 0.543
0.162IleCys: 0.162 ± 0.121
2.588IleAsp: 2.588 ± 0.433
2.184IleGlu: 2.184 ± 0.448
0.89IlePhe: 0.89 ± 0.291
2.507IleGly: 2.507 ± 0.492
0.971IleHis: 0.971 ± 0.476
0.728IleIle: 0.728 ± 0.313
2.507IleLys: 2.507 ± 0.408
2.184IleLeu: 2.184 ± 0.388
0.809IleMet: 0.809 ± 0.272
0.647IleAsn: 0.647 ± 0.205
2.103IlePro: 2.103 ± 0.44
1.375IleGln: 1.375 ± 0.363
3.235IleArg: 3.235 ± 0.629
1.537IleSer: 1.537 ± 0.29
3.073IleThr: 3.073 ± 0.681
2.588IleVal: 2.588 ± 0.432
0.566IleTrp: 0.566 ± 0.208
0.809IleTyr: 0.809 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
5.015LysAla: 5.015 ± 0.695
0.485LysCys: 0.485 ± 0.262
2.103LysAsp: 2.103 ± 0.286
2.507LysGlu: 2.507 ± 0.453
0.971LysPhe: 0.971 ± 0.307
3.397LysGly: 3.397 ± 0.562
1.132LysHis: 1.132 ± 0.323
1.132LysIle: 1.132 ± 0.339
1.941LysLys: 1.941 ± 0.521
3.801LysLeu: 3.801 ± 0.558
1.051LysMet: 1.051 ± 0.28
1.294LysAsn: 1.294 ± 0.35
3.559LysPro: 3.559 ± 0.709
0.566LysGln: 0.566 ± 0.255
4.125LysArg: 4.125 ± 0.655
2.588LysSer: 2.588 ± 0.501
3.154LysThr: 3.154 ± 0.688
3.478LysVal: 3.478 ± 0.647
0.728LysTrp: 0.728 ± 0.334
1.051LysTyr: 1.051 ± 0.317
0.0LysXaa: 0.0 ± 0.0
Leu
7.845LeuAla: 7.845 ± 0.989
0.647LeuCys: 0.647 ± 0.221
6.147LeuAsp: 6.147 ± 0.75
4.044LeuGlu: 4.044 ± 0.91
2.669LeuPhe: 2.669 ± 0.449
5.985LeuGly: 5.985 ± 0.715
0.971LeuHis: 0.971 ± 0.289
3.801LeuIle: 3.801 ± 0.64
4.125LeuLys: 4.125 ± 0.612
5.257LeuLeu: 5.257 ± 0.667
1.132LeuMet: 1.132 ± 0.274
2.993LeuAsn: 2.993 ± 0.521
5.823LeuPro: 5.823 ± 0.574
2.184LeuGln: 2.184 ± 0.483
6.39LeuArg: 6.39 ± 0.9
5.904LeuSer: 5.904 ± 0.84
5.904LeuThr: 5.904 ± 0.691
5.662LeuVal: 5.662 ± 0.71
0.89LeuTrp: 0.89 ± 0.263
1.618LeuTyr: 1.618 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
3.64MetAla: 3.64 ± 0.528
0.243MetCys: 0.243 ± 0.146
1.375MetAsp: 1.375 ± 0.295
0.728MetGlu: 0.728 ± 0.261
0.404MetPhe: 0.404 ± 0.223
2.75MetGly: 2.75 ± 0.465
0.485MetHis: 0.485 ± 0.169
1.132MetIle: 1.132 ± 0.305
1.294MetLys: 1.294 ± 0.312
1.698MetLeu: 1.698 ± 0.3
0.243MetMet: 0.243 ± 0.139
0.566MetAsn: 0.566 ± 0.176
1.456MetPro: 1.456 ± 0.343
0.566MetGln: 0.566 ± 0.189
1.779MetArg: 1.779 ± 0.418
1.132MetSer: 1.132 ± 0.264
1.779MetThr: 1.779 ± 0.303
0.971MetVal: 0.971 ± 0.294
0.162MetTrp: 0.162 ± 0.094
0.485MetTyr: 0.485 ± 0.28
0.0MetXaa: 0.0 ± 0.0
Asn
4.044AsnAla: 4.044 ± 0.521
0.081AsnCys: 0.081 ± 0.075
1.375AsnAsp: 1.375 ± 0.3
2.103AsnGlu: 2.103 ± 0.431
0.971AsnPhe: 0.971 ± 0.294
4.044AsnGly: 4.044 ± 0.591
0.485AsnHis: 0.485 ± 0.187
1.051AsnIle: 1.051 ± 0.338
1.698AsnLys: 1.698 ± 0.341
1.779AsnLeu: 1.779 ± 0.31
0.566AsnMet: 0.566 ± 0.2
0.647AsnAsn: 0.647 ± 0.238
2.265AsnPro: 2.265 ± 0.477
0.809AsnGln: 0.809 ± 0.311
1.456AsnArg: 1.456 ± 0.399
1.537AsnSer: 1.537 ± 0.377
1.375AsnThr: 1.375 ± 0.291
2.265AsnVal: 2.265 ± 0.346
0.404AsnTrp: 0.404 ± 0.211
0.485AsnTyr: 0.485 ± 0.202
0.0AsnXaa: 0.0 ± 0.0
Pro
6.228ProAla: 6.228 ± 0.821
0.647ProCys: 0.647 ± 0.243
4.287ProAsp: 4.287 ± 0.687
5.419ProGlu: 5.419 ± 1.121
1.698ProPhe: 1.698 ± 0.379
4.934ProGly: 4.934 ± 0.844
0.809ProHis: 0.809 ± 0.259
1.537ProIle: 1.537 ± 0.336
2.588ProLys: 2.588 ± 0.452
2.588ProLeu: 2.588 ± 0.522
1.375ProMet: 1.375 ± 0.294
1.698ProAsn: 1.698 ± 0.373
1.537ProPro: 1.537 ± 0.583
1.213ProGln: 1.213 ± 0.311
2.426ProArg: 2.426 ± 0.402
3.154ProSer: 3.154 ± 0.675
4.448ProThr: 4.448 ± 0.639
4.368ProVal: 4.368 ± 0.663
0.728ProTrp: 0.728 ± 0.199
1.375ProTyr: 1.375 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
2.669GlnAla: 2.669 ± 0.566
0.404GlnCys: 0.404 ± 0.159
1.456GlnAsp: 1.456 ± 0.412
1.375GlnGlu: 1.375 ± 0.283
1.375GlnPhe: 1.375 ± 0.355
2.507GlnGly: 2.507 ± 0.374
0.647GlnHis: 0.647 ± 0.287
0.809GlnIle: 0.809 ± 0.215
0.89GlnLys: 0.89 ± 0.274
2.346GlnLeu: 2.346 ± 0.408
0.566GlnMet: 0.566 ± 0.203
1.132GlnAsn: 1.132 ± 0.319
1.375GlnPro: 1.375 ± 0.354
0.485GlnGln: 0.485 ± 0.236
2.588GlnArg: 2.588 ± 0.5
1.132GlnSer: 1.132 ± 0.294
2.103GlnThr: 2.103 ± 0.323
2.75GlnVal: 2.75 ± 0.421
0.647GlnTrp: 0.647 ± 0.244
0.647GlnTyr: 0.647 ± 0.244
0.0GlnXaa: 0.0 ± 0.0
Arg
8.088ArgAla: 8.088 ± 1.066
0.566ArgCys: 0.566 ± 0.258
4.529ArgAsp: 4.529 ± 0.831
3.801ArgGlu: 3.801 ± 0.596
3.235ArgPhe: 3.235 ± 0.52
5.5ArgGly: 5.5 ± 0.661
1.618ArgHis: 1.618 ± 0.491
3.073ArgIle: 3.073 ± 0.523
4.044ArgLys: 4.044 ± 0.545
4.853ArgLeu: 4.853 ± 0.681
1.779ArgMet: 1.779 ± 0.426
2.588ArgAsn: 2.588 ± 0.413
1.941ArgPro: 1.941 ± 0.543
1.779ArgGln: 1.779 ± 0.412
4.529ArgArg: 4.529 ± 0.642
3.235ArgSer: 3.235 ± 0.638
4.044ArgThr: 4.044 ± 0.63
5.823ArgVal: 5.823 ± 0.791
1.698ArgTrp: 1.698 ± 0.482
2.022ArgTyr: 2.022 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
8.169SerAla: 8.169 ± 0.732
0.324SerCys: 0.324 ± 0.158
3.64SerAsp: 3.64 ± 0.542
2.426SerGlu: 2.426 ± 0.451
1.698SerPhe: 1.698 ± 0.364
4.934SerGly: 4.934 ± 0.562
1.213SerHis: 1.213 ± 0.339
1.941SerIle: 1.941 ± 0.342
2.75SerLys: 2.75 ± 0.486
4.934SerLeu: 4.934 ± 0.676
1.294SerMet: 1.294 ± 0.273
1.698SerAsn: 1.698 ± 0.408
3.235SerPro: 3.235 ± 0.498
1.213SerGln: 1.213 ± 0.303
2.265SerArg: 2.265 ± 0.437
2.912SerSer: 2.912 ± 0.647
3.882SerThr: 3.882 ± 0.584
4.368SerVal: 4.368 ± 0.75
1.213SerTrp: 1.213 ± 0.263
0.971SerTyr: 0.971 ± 0.316
0.0SerXaa: 0.0 ± 0.0
Thr
7.926ThrAla: 7.926 ± 0.738
0.566ThrCys: 0.566 ± 0.252
3.801ThrAsp: 3.801 ± 0.65
3.801ThrGlu: 3.801 ± 0.539
2.426ThrPhe: 2.426 ± 0.375
6.713ThrGly: 6.713 ± 0.911
0.971ThrHis: 0.971 ± 0.297
2.265ThrIle: 2.265 ± 0.373
2.669ThrLys: 2.669 ± 0.364
5.419ThrLeu: 5.419 ± 0.51
1.132ThrMet: 1.132 ± 0.305
1.941ThrAsn: 1.941 ± 0.355
3.963ThrPro: 3.963 ± 0.719
2.022ThrGln: 2.022 ± 0.523
4.044ThrArg: 4.044 ± 0.579
3.478ThrSer: 3.478 ± 0.532
5.742ThrThr: 5.742 ± 1.03
6.632ThrVal: 6.632 ± 0.788
1.375ThrTrp: 1.375 ± 0.365
2.184ThrTyr: 2.184 ± 0.449
0.0ThrXaa: 0.0 ± 0.0
Val
9.22ValAla: 9.22 ± 1.162
0.647ValCys: 0.647 ± 0.244
5.904ValAsp: 5.904 ± 0.687
4.125ValGlu: 4.125 ± 0.707
2.426ValPhe: 2.426 ± 0.47
6.632ValGly: 6.632 ± 0.91
1.375ValHis: 1.375 ± 0.368
2.993ValIle: 2.993 ± 0.668
2.265ValLys: 2.265 ± 0.379
5.581ValLeu: 5.581 ± 0.641
1.698ValMet: 1.698 ± 0.424
2.75ValAsn: 2.75 ± 0.583
4.044ValPro: 4.044 ± 0.6
2.426ValGln: 2.426 ± 0.535
5.742ValArg: 5.742 ± 0.693
4.529ValSer: 4.529 ± 0.523
6.875ValThr: 6.875 ± 0.827
5.742ValVal: 5.742 ± 0.638
1.456ValTrp: 1.456 ± 0.378
1.779ValTyr: 1.779 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
2.184TrpAla: 2.184 ± 0.34
0.404TrpCys: 0.404 ± 0.186
0.809TrpAsp: 0.809 ± 0.27
1.051TrpGlu: 1.051 ± 0.298
0.728TrpPhe: 0.728 ± 0.286
1.456TrpGly: 1.456 ± 0.329
0.243TrpHis: 0.243 ± 0.161
0.566TrpIle: 0.566 ± 0.21
0.485TrpLys: 0.485 ± 0.251
2.103TrpLeu: 2.103 ± 0.324
0.324TrpMet: 0.324 ± 0.178
0.324TrpAsn: 0.324 ± 0.161
0.324TrpPro: 0.324 ± 0.213
1.051TrpGln: 1.051 ± 0.266
1.941TrpArg: 1.941 ± 0.493
1.213TrpSer: 1.213 ± 0.29
1.456TrpThr: 1.456 ± 0.321
1.698TrpVal: 1.698 ± 0.487
0.081TrpTrp: 0.081 ± 0.078
0.243TrpTyr: 0.243 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.559TyrAla: 3.559 ± 0.556
0.243TyrCys: 0.243 ± 0.147
2.103TyrAsp: 2.103 ± 0.361
1.86TyrGlu: 1.86 ± 0.282
0.404TyrPhe: 0.404 ± 0.22
2.669TyrGly: 2.669 ± 0.435
0.404TyrHis: 0.404 ± 0.161
0.081TyrIle: 0.081 ± 0.082
0.809TyrLys: 0.809 ± 0.282
1.051TyrLeu: 1.051 ± 0.275
0.647TyrMet: 0.647 ± 0.295
0.404TyrAsn: 0.404 ± 0.196
0.728TyrPro: 0.728 ± 0.27
0.485TyrGln: 0.485 ± 0.181
2.507TyrArg: 2.507 ± 0.655
1.294TyrSer: 1.294 ± 0.359
1.213TyrThr: 1.213 ± 0.351
2.507TyrVal: 2.507 ± 0.446
0.324TyrTrp: 0.324 ± 0.151
0.324TyrTyr: 0.324 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12365 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski