Luteimonas aestuarii
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3335 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R5U4Q5|A0A4R5U4Q5_9GAMM Right-handed parallel beta-helix repeat-containing protein OS=Luteimonas aestuarii OX=453837 GN=E2F46_00415 PE=4 SV=1
MM1 pKa = 7.49 TIKK4 pKa = 9.97 TYY6 pKa = 10.4 TPAEE10 pKa = 4.59 AIQAAQAEE18 pKa = 4.77 GCIEE22 pKa = 3.89 IAAGVHH28 pKa = 6.52 LSSQEE33 pKa = 4.08 NIVADD38 pKa = 3.58 QDD40 pKa = 3.6 DD41 pKa = 4.03 WSVEE45 pKa = 3.64 GDD47 pKa = 3.44 QMHH50 pKa = 7.74 DD51 pKa = 3.63 FTKK54 pKa = 10.47 APYY57 pKa = 9.58 WITTNDD63 pKa = 3.53 GQVQPIYY70 pKa = 11.44 GMDD73 pKa = 3.97 DD74 pKa = 3.34 KK75 pKa = 11.84 DD76 pKa = 5.72 LIDD79 pKa = 3.72 VLANAA84 pKa = 5.26
Molecular weight: 9.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.783
IPC_protein 3.732
Toseland 3.528
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.859
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A4R5TXZ5|A0A4R5TXZ5_9GAMM MPT synthase subunit 2 OS=Luteimonas aestuarii OX=453837 GN=E2F46_05525 PE=3 SV=1
MM1 pKa = 6.08 TQARR5 pKa = 11.84 RR6 pKa = 11.84 TRR8 pKa = 11.84 SKK10 pKa = 10.45 EE11 pKa = 3.76 RR12 pKa = 11.84 GNGRR16 pKa = 11.84 PAPPAAADD24 pKa = 3.42 ARR26 pKa = 11.84 EE27 pKa = 4.05 AGLLYY32 pKa = 10.61 VDD34 pKa = 5.49 DD35 pKa = 4.69 SAPGIHH41 pKa = 7.0 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 CGRR47 pKa = 11.84 GFRR50 pKa = 11.84 YY51 pKa = 10.39 DD52 pKa = 3.87 DD53 pKa = 3.41 ATGVCIRR60 pKa = 11.84 DD61 pKa = 3.38 EE62 pKa = 4.16 ATLARR67 pKa = 11.84 IRR69 pKa = 11.84 ALAIPPAYY77 pKa = 10.14 VDD79 pKa = 3.15 VWICASPRR87 pKa = 11.84 GHH89 pKa = 6.67 LQATGRR95 pKa = 11.84 DD96 pKa = 3.29 ARR98 pKa = 11.84 GRR100 pKa = 11.84 KK101 pKa = 7.65 QYY103 pKa = 10.33 RR104 pKa = 11.84 YY105 pKa = 9.59 HH106 pKa = 6.99 PDD108 pKa = 2.62 WRR110 pKa = 11.84 STRR113 pKa = 11.84 DD114 pKa = 2.81 ADD116 pKa = 4.39 KK117 pKa = 10.68 FDD119 pKa = 4.01 RR120 pKa = 11.84 LLAFGKK126 pKa = 10.15 ALPRR130 pKa = 11.84 LRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 LRR136 pKa = 11.84 IDD138 pKa = 3.48 LSLRR142 pKa = 11.84 GLPRR146 pKa = 11.84 EE147 pKa = 4.0 KK148 pKa = 10.74 ALAALVTIMATTLMRR163 pKa = 11.84 IGNDD167 pKa = 2.6 EE168 pKa = 3.99 YY169 pKa = 11.64 ARR171 pKa = 11.84 DD172 pKa = 3.74 NGSYY176 pKa = 11.32 GLTTLRR182 pKa = 11.84 NRR184 pKa = 11.84 HH185 pKa = 5.46 AKK187 pKa = 9.52 FLRR190 pKa = 11.84 DD191 pKa = 3.45 GLHH194 pKa = 6.18 LQFKK198 pKa = 9.93 GKK200 pKa = 10.13 GGKK203 pKa = 6.86 THH205 pKa = 7.65 DD206 pKa = 4.04 AGIDD210 pKa = 3.3 DD211 pKa = 4.07 RR212 pKa = 11.84 RR213 pKa = 11.84 LVKK216 pKa = 10.46 IMRR219 pKa = 11.84 AMHH222 pKa = 6.49 EE223 pKa = 4.27 LPGQRR228 pKa = 11.84 LFQYY232 pKa = 10.7 RR233 pKa = 11.84 DD234 pKa = 3.28 DD235 pKa = 4.38 EE236 pKa = 5.0 GNLRR240 pKa = 11.84 PLDD243 pKa = 3.87 SSDD246 pKa = 3.4 ANDD249 pKa = 3.66 YY250 pKa = 10.83 LRR252 pKa = 11.84 EE253 pKa = 3.98 RR254 pKa = 11.84 MGGPFTAKK262 pKa = 10.49 DD263 pKa = 3.53 FRR265 pKa = 11.84 TWGATATAFRR275 pKa = 11.84 LLAATPVPEE284 pKa = 5.11 DD285 pKa = 3.13 ATKK288 pKa = 10.62 RR289 pKa = 11.84 DD290 pKa = 3.35 IAGVEE295 pKa = 3.71 RR296 pKa = 11.84 DD297 pKa = 3.76 VVNAVAEE304 pKa = 4.27 MLGNTPAVCRR314 pKa = 11.84 KK315 pKa = 10.15 SYY317 pKa = 10.75 IDD319 pKa = 3.38 PCVFDD324 pKa = 3.77 GWQDD328 pKa = 3.43 GRR330 pKa = 11.84 LHH332 pKa = 6.97 RR333 pKa = 11.84 AAQGARR339 pKa = 11.84 GPRR342 pKa = 11.84 QWEE345 pKa = 4.15 TATLRR350 pKa = 11.84 YY351 pKa = 9.05 LRR353 pKa = 11.84 RR354 pKa = 11.84 VKK356 pKa = 10.62 KK357 pKa = 10.17 PSRR360 pKa = 3.51
Molecular weight: 40.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.604
IPC_protein 10.555
Toseland 10.613
ProMoST 10.482
Dawson 10.716
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.804
Grimsley 10.789
Solomon 10.862
Lehninger 10.818
Nozaki 10.599
DTASelect 10.467
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.467
IPC_peptide 10.862
IPC2_peptide 9.589
IPC2.peptide.svr19 8.649
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3335
0
3335
1091155
22
2104
327.2
35.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.252 ± 0.064
0.824 ± 0.014
6.277 ± 0.036
5.297 ± 0.035
3.382 ± 0.024
8.723 ± 0.038
2.328 ± 0.022
4.023 ± 0.033
2.4 ± 0.038
10.559 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.245 ± 0.02
2.351 ± 0.028
5.401 ± 0.031
3.488 ± 0.028
8.098 ± 0.047
4.994 ± 0.027
4.966 ± 0.033
7.624 ± 0.041
1.55 ± 0.019
2.218 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here