Gordonia phage Suerte
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8DDD4|A0A5P8DDD4_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage Suerte OX=2652883 GN=35 PE=4 SV=1
MM1 pKa = 7.11 TAPEE5 pKa = 4.48 RR6 pKa = 11.84 MTPLASGVEE15 pKa = 4.07 NGVRR19 pKa = 11.84 WVTCVAPIYY28 pKa = 10.31 GAVNGYY34 pKa = 9.27 VLLPEE39 pKa = 3.98 GHH41 pKa = 7.18 PWRR44 pKa = 11.84 GLDD47 pKa = 3.35 YY48 pKa = 11.06 DD49 pKa = 3.88 QIDD52 pKa = 4.08 VEE54 pKa = 4.55 VHH56 pKa = 5.95 GGLTYY61 pKa = 10.9 GHH63 pKa = 7.31 DD64 pKa = 3.15 GWIGFDD70 pKa = 3.44 TLHH73 pKa = 7.13 AGDD76 pKa = 4.32 VWPGSPQYY84 pKa = 10.73 GGPRR88 pKa = 11.84 PYY90 pKa = 11.42 DD91 pKa = 3.48 MRR93 pKa = 11.84 WTAEE97 pKa = 3.92 MVAEE101 pKa = 4.32 EE102 pKa = 4.33 ARR104 pKa = 11.84 RR105 pKa = 11.84 LAARR109 pKa = 11.84 VAAAAEE115 pKa = 4.37 TTDD118 pKa = 3.63 TGPVSGPPYY127 pKa = 10.27 PPDD130 pKa = 3.82 RR131 pKa = 11.84 VPDD134 pKa = 3.76 DD135 pKa = 4.8 HH136 pKa = 8.05 DD137 pKa = 5.22 DD138 pKa = 3.6 GTMPTNVAEE147 pKa = 4.5 FAEE150 pKa = 4.44 QVVGRR155 pKa = 11.84 RR156 pKa = 11.84 IIKK159 pKa = 10.19 AEE161 pKa = 3.85 KK162 pKa = 9.13 TDD164 pKa = 4.14 FRR166 pKa = 11.84 PEE168 pKa = 4.11 DD169 pKa = 3.63 FDD171 pKa = 4.21 GTEE174 pKa = 3.61 HH175 pKa = 6.72 TYY177 pKa = 9.33 WSSYY181 pKa = 10.55 SDD183 pKa = 3.16 THH185 pKa = 7.87 GLVLTLDD192 pKa = 3.67 DD193 pKa = 3.62 GRR195 pKa = 11.84 RR196 pKa = 11.84 VVLVDD201 pKa = 3.84 TSDD204 pKa = 3.51 CCAYY208 pKa = 9.81 TSLEE212 pKa = 4.01 EE213 pKa = 4.45 FFLHH217 pKa = 6.73 PEE219 pKa = 4.11 RR220 pKa = 11.84 VDD222 pKa = 3.54 HH223 pKa = 6.71 VITGVATTDD232 pKa = 3.57 GYY234 pKa = 10.89 EE235 pKa = 3.94 RR236 pKa = 11.84 WHH238 pKa = 6.21 VFADD242 pKa = 3.42 LGDD245 pKa = 3.76 ILEE248 pKa = 4.77 LKK250 pKa = 10.3 IGWSAGNPFYY260 pKa = 10.93 YY261 pKa = 10.41 GYY263 pKa = 10.5 GFNIGVSNVIEE274 pKa = 4.5 GSLAQPEE281 pKa = 4.53 LEE283 pKa = 4.24 QGQSS287 pKa = 2.93
Molecular weight: 31.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.248
IPC2_protein 4.38
IPC_protein 4.355
Toseland 4.177
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.215
Rodwell 4.19
Grimsley 4.088
Solomon 4.317
Lehninger 4.279
Nozaki 4.431
DTASelect 4.622
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.469
Patrickios 3.032
IPC_peptide 4.317
IPC2_peptide 4.457
IPC2.peptide.svr19 4.387
Protein with the highest isoelectric point:
>tr|A0A5P8DDH2|A0A5P8DDH2_9CAUD Tail assembly chaperone OS=Gordonia phage Suerte OX=2652883 GN=15 PE=4 SV=1
MM1 pKa = 7.59 ARR3 pKa = 11.84 PPLPLGTWGKK13 pKa = 8.09 ITRR16 pKa = 11.84 KK17 pKa = 7.83 EE18 pKa = 4.03 TAPGRR23 pKa = 11.84 WRR25 pKa = 11.84 ARR27 pKa = 11.84 AQYY30 pKa = 10.63 RR31 pKa = 11.84 DD32 pKa = 3.45 FDD34 pKa = 4.04 GVTRR38 pKa = 11.84 PVEE41 pKa = 4.1 RR42 pKa = 11.84 YY43 pKa = 8.93 GKK45 pKa = 10.11 SGAAAEE51 pKa = 4.2 RR52 pKa = 11.84 ALVEE56 pKa = 4.04 ALRR59 pKa = 11.84 DD60 pKa = 3.35 RR61 pKa = 11.84 AAARR65 pKa = 11.84 GDD67 pKa = 3.48 IINRR71 pKa = 11.84 DD72 pKa = 3.34 TTIAALATAWIASRR86 pKa = 11.84 VAEE89 pKa = 4.3 DD90 pKa = 3.47 ALRR93 pKa = 11.84 EE94 pKa = 3.98 QSVEE98 pKa = 4.09 QYY100 pKa = 10.59 QSAIDD105 pKa = 3.64 KK106 pKa = 10.6 HH107 pKa = 6.23 IVPALGRR114 pKa = 11.84 VRR116 pKa = 11.84 IGEE119 pKa = 4.26 VGVGILDD126 pKa = 3.71 RR127 pKa = 11.84 FLEE130 pKa = 5.37 GISSAAVAKK139 pKa = 9.94 RR140 pKa = 11.84 CRR142 pKa = 11.84 VVLTGMFGLAARR154 pKa = 11.84 HH155 pKa = 6.19 DD156 pKa = 5.3 AIDD159 pKa = 3.87 HH160 pKa = 5.53 NPVRR164 pKa = 11.84 EE165 pKa = 4.24 TVSRR169 pKa = 11.84 AQKK172 pKa = 9.78 RR173 pKa = 11.84 IPVRR177 pKa = 11.84 AMTVGEE183 pKa = 4.3 VAVMRR188 pKa = 11.84 ARR190 pKa = 11.84 VAAWAGANASGPPRR204 pKa = 11.84 GQDD207 pKa = 3.65 LPDD210 pKa = 4.39 ILDD213 pKa = 3.64 AMLGTGARR221 pKa = 11.84 IGEE224 pKa = 3.98 VLAIRR229 pKa = 11.84 KK230 pKa = 8.91 EE231 pKa = 3.99 DD232 pKa = 3.52 LDD234 pKa = 4.2 LRR236 pKa = 11.84 SNPPTVVITGTIVGNKK252 pKa = 8.52 RR253 pKa = 11.84 QPAPKK258 pKa = 8.67 TDD260 pKa = 3.19 YY261 pKa = 10.73 SYY263 pKa = 11.55 RR264 pKa = 11.84 RR265 pKa = 11.84 LILPSFVAAALRR277 pKa = 11.84 RR278 pKa = 11.84 QLARR282 pKa = 11.84 NLPTDD287 pKa = 4.05 DD288 pKa = 3.8 LHH290 pKa = 9.05 LVFPSRR296 pKa = 11.84 TGGPRR301 pKa = 11.84 MGNNVRR307 pKa = 11.84 RR308 pKa = 11.84 QLRR311 pKa = 11.84 EE312 pKa = 3.62 ARR314 pKa = 11.84 GDD316 pKa = 3.57 DD317 pKa = 4.09 FDD319 pKa = 3.91 WVTPKK324 pKa = 10.51 VYY326 pKa = 10.5 RR327 pKa = 11.84 KK328 pKa = 7.56 TVATAIDD335 pKa = 3.53 RR336 pKa = 11.84 AADD339 pKa = 3.11 IEE341 pKa = 4.41 TAARR345 pKa = 11.84 QLGHH349 pKa = 6.24 SRR351 pKa = 11.84 STTTQAHH358 pKa = 4.56 YY359 pKa = 10.73 VEE361 pKa = 4.99 RR362 pKa = 11.84 LLVAPDD368 pKa = 3.29 VRR370 pKa = 11.84 DD371 pKa = 3.52 VLDD374 pKa = 3.44 EE375 pKa = 4.46 FGPVSRR381 pKa = 11.84 GFSVGDD387 pKa = 3.33 VSS389 pKa = 4.72
Molecular weight: 42.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.502
IPC_protein 10.511
Toseland 10.628
ProMoST 10.438
Dawson 10.716
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.745
Grimsley 10.774
Solomon 10.891
Lehninger 10.847
Nozaki 10.599
DTASelect 10.482
Thurlkill 10.628
EMBOSS 11.052
Sillero 10.657
Patrickios 10.423
IPC_peptide 10.891
IPC2_peptide 9.443
IPC2.peptide.svr19 8.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
14950
38
2137
202.0
21.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.575 ± 0.548
0.696 ± 0.124
6.749 ± 0.407
5.579 ± 0.252
2.408 ± 0.162
8.415 ± 0.46
1.96 ± 0.2
4.589 ± 0.204
3.157 ± 0.255
7.819 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.047 ± 0.124
3.043 ± 0.24
5.679 ± 0.284
3.726 ± 0.328
7.505 ± 0.467
5.344 ± 0.229
7.344 ± 0.27
7.324 ± 0.293
1.853 ± 0.144
2.187 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here