Desulfitobacterium dichloroeliminans (strain LMG P-21439 / DCA1)
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3294 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0F8G0|L0F8G0_DESDL ABC-type multidrug transport system ATPase component OS=Desulfitobacterium dichloroeliminans (strain LMG P-21439 / DCA1) OX=871963 GN=Desdi_1443 PE=4 SV=1
MM1 pKa = 7.68 SDD3 pKa = 3.6 VIGIPNPPPGTCLPWEE19 pKa = 5.35 KK20 pKa = 9.39 ITEE23 pKa = 4.48 DD24 pKa = 3.51 YY25 pKa = 10.63 GPPVGDD31 pKa = 3.37 EE32 pKa = 4.45 ALLKK36 pKa = 9.86 GQWEE40 pKa = 4.25 EE41 pKa = 4.08 LDD43 pKa = 3.53 ALAYY47 pKa = 10.47 LYY49 pKa = 10.29 LWWWVQRR56 pKa = 3.88
Molecular weight: 6.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.05
IPC_protein 3.872
Toseland 3.694
ProMoST 4.037
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.617
Solomon 3.821
Lehninger 3.77
Nozaki 3.999
DTASelect 4.113
Thurlkill 3.77
EMBOSS 3.795
Sillero 3.986
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|L0F2N9|L0F2N9_DESDL Uncharacterized protein OS=Desulfitobacterium dichloroeliminans (strain LMG P-21439 / DCA1) OX=871963 GN=Desdi_0561 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.49 RR3 pKa = 11.84 TWQPKK8 pKa = 7.14 NRR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 4.85 QRR14 pKa = 11.84 VHH16 pKa = 6.14 GFLSRR21 pKa = 11.84 MSSSTGRR28 pKa = 11.84 NVLKK32 pKa = 10.5 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.19 GRR39 pKa = 11.84 KK40 pKa = 8.79 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3294
0
3294
1018540
29
2872
309.2
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.745 ± 0.043
1.079 ± 0.019
4.971 ± 0.028
7.234 ± 0.045
4.025 ± 0.031
7.469 ± 0.041
1.893 ± 0.021
7.296 ± 0.047
6.031 ± 0.034
10.35 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.714 ± 0.021
3.944 ± 0.029
4.013 ± 0.028
3.915 ± 0.033
4.69 ± 0.033
5.896 ± 0.036
5.253 ± 0.037
7.069 ± 0.032
1.079 ± 0.017
3.336 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here