Sphaceloma murrayae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Myriangiales; Elsinoaceae; Sphaceloma

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K1QJF5|A0A2K1QJF5_9PEZI Separase OS=Sphaceloma murrayae OX=2082308 GN=CAC42_8277 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84VLAASLLFAASALAQAPDD20 pKa = 3.47ADD22 pKa = 4.42IITVAGLGPAGDD34 pKa = 3.95EE35 pKa = 4.92SNFQATFLGIQDD47 pKa = 4.83DD48 pKa = 4.4VPSYY52 pKa = 11.52DD53 pKa = 3.59VTCTGTTDD61 pKa = 3.22YY62 pKa = 10.55CANGGFILSTDD73 pKa = 3.53PEE75 pKa = 5.03GYY77 pKa = 10.8NIDD80 pKa = 3.83TDD82 pKa = 3.73VDD84 pKa = 3.47GDD86 pKa = 4.17GYY88 pKa = 11.02LEE90 pKa = 4.09KK91 pKa = 10.42TSLCFVNAGTASCDD105 pKa = 3.11IEE107 pKa = 5.56IYY109 pKa = 9.87ATDD112 pKa = 3.86DD113 pKa = 3.69TLLSTAAPEE122 pKa = 4.32TRR124 pKa = 11.84SGTITLTVSPGTTTMGEE141 pKa = 4.53GPVSTTRR148 pKa = 11.84TVDD151 pKa = 3.39VPITSTLRR159 pKa = 11.84STVTVPSAGTALPTYY174 pKa = 9.35AAPSGQDD181 pKa = 2.93TSASVGVPTSLTTGRR196 pKa = 11.84NLTAPTSTRR205 pKa = 11.84PNAPVFTGAASGKK218 pKa = 9.93RR219 pKa = 11.84VGDD222 pKa = 3.63VAGGTMGLVVAGVLGVAGLAFMLL245 pKa = 4.8

Molecular weight: 24.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K1QX11|A0A2K1QX11_9PEZI Phosducin domain-containing protein OS=Sphaceloma murrayae OX=2082308 GN=CAC42_7440 PE=3 SV=1
MM1 pKa = 7.16FRR3 pKa = 11.84SRR5 pKa = 11.84RR6 pKa = 11.84NAAPVGTTHH15 pKa = 7.34RR16 pKa = 11.84PAPATRR22 pKa = 11.84SKK24 pKa = 10.45PSLMQRR30 pKa = 11.84LRR32 pKa = 11.84GSKK35 pKa = 10.22AQTTTTTKK43 pKa = 10.26HH44 pKa = 4.34SHH46 pKa = 5.36NPITGTHH53 pKa = 5.1TTTHH57 pKa = 5.45TTHH60 pKa = 6.54GTPHH64 pKa = 6.39HH65 pKa = 6.01TTHH68 pKa = 7.01KK69 pKa = 10.3APRR72 pKa = 11.84SSRR75 pKa = 11.84RR76 pKa = 11.84TRR78 pKa = 11.84RR79 pKa = 11.84TAATTTTTAPAHH91 pKa = 4.94HH92 pKa = 6.77HH93 pKa = 5.96RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84PSLGDD101 pKa = 3.38KK102 pKa = 10.35MSGAMMKK109 pKa = 9.81IRR111 pKa = 11.84GSLTRR116 pKa = 11.84RR117 pKa = 11.84PGLKK121 pKa = 10.1AAGTRR126 pKa = 11.84RR127 pKa = 11.84MHH129 pKa = 5.65GTDD132 pKa = 3.13GRR134 pKa = 11.84GARR137 pKa = 11.84YY138 pKa = 9.2

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8255

0

8255

4222183

41

5672

511.5

56.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.99 ± 0.022

1.05 ± 0.01

5.834 ± 0.019

6.13 ± 0.028

3.494 ± 0.017

7.268 ± 0.026

2.273 ± 0.013

4.574 ± 0.018

4.883 ± 0.027

8.596 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.011

3.421 ± 0.013

6.074 ± 0.03

4.06 ± 0.02

6.4 ± 0.026

8.358 ± 0.035

6.234 ± 0.024

6.185 ± 0.019

1.357 ± 0.009

2.641 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski