Eptesicus fuscus gammaherpesvirus
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0ZNY5|A0A2D0ZNY5_9GAMA Uncharacterized protein OS=Eptesicus fuscus gammaherpesvirus OX=2035399 PE=4 SV=1
MM1 pKa = 7.03 ATAVPIWGVSEE12 pKa = 4.43 ADD14 pKa = 3.29 GAEE17 pKa = 3.97 WNNLIEE23 pKa = 4.52 LGVRR27 pKa = 11.84 NGSQMEE33 pKa = 4.11 RR34 pKa = 11.84 AVSILRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 LYY44 pKa = 8.9 NTEE47 pKa = 3.73 PLGYY51 pKa = 9.78 LASLLALRR59 pKa = 11.84 QEE61 pKa = 4.59 LNSVTGGSAQEE72 pKa = 4.01 ILLEE76 pKa = 4.11 SLAVIQQLAKK86 pKa = 10.56 VIYY89 pKa = 10.42 GEE91 pKa = 5.68 LKK93 pKa = 10.45 DD94 pKa = 3.87 QPPTARR100 pKa = 11.84 INEE103 pKa = 4.01 IFRR106 pKa = 11.84 DD107 pKa = 3.73 CRR109 pKa = 11.84 LRR111 pKa = 11.84 LALVLDD117 pKa = 4.71 AACGCVDD124 pKa = 4.33 CFRR127 pKa = 11.84 LAKK130 pKa = 9.37 VLKK133 pKa = 9.67 VAPRR137 pKa = 11.84 CLRR140 pKa = 11.84 PPKK143 pKa = 10.56 LMAHH147 pKa = 6.5 EE148 pKa = 4.42 RR149 pKa = 11.84 QCAAASTLTYY159 pKa = 9.8 FYY161 pKa = 10.66 NSYY164 pKa = 9.43 MVAGSDD170 pKa = 3.64 VEE172 pKa = 4.3 VAPEE176 pKa = 4.44 HH177 pKa = 7.13 IEE179 pKa = 3.98 LLLIRR184 pKa = 11.84 PDD186 pKa = 3.53 QLNSYY191 pKa = 9.32 PYY193 pKa = 10.38 GWDD196 pKa = 3.35 EE197 pKa = 4.04 EE198 pKa = 4.9 VAGLMSCLNLCWLYY212 pKa = 11.12 TMMYY216 pKa = 8.73 EE217 pKa = 3.84 HH218 pKa = 7.57 ATRR221 pKa = 11.84 GMCTLTRR228 pKa = 11.84 ALEE231 pKa = 4.07 EE232 pKa = 4.22 AYY234 pKa = 10.52 HH235 pKa = 6.03 RR236 pKa = 11.84 VRR238 pKa = 11.84 PGSKK242 pKa = 8.73 ATLEE246 pKa = 4.07 NMVQAVIEE254 pKa = 4.4 GDD256 pKa = 3.49 SRR258 pKa = 11.84 ASRR261 pKa = 11.84 SEE263 pKa = 3.75 EE264 pKa = 3.97 PRR266 pKa = 11.84 PHH268 pKa = 7.22 HH269 pKa = 5.55 YY270 pKa = 9.61 TLIKK274 pKa = 10.15 PLVTMKK280 pKa = 10.62 HH281 pKa = 3.82 EE282 pKa = 4.51 HH283 pKa = 6.13 RR284 pKa = 11.84 WSEE287 pKa = 4.12 KK288 pKa = 9.53 QDD290 pKa = 3.43 SAVQTQVSCLYY301 pKa = 9.88 EE302 pKa = 4.2 LVKK305 pKa = 10.84 GMYY308 pKa = 9.89 GPYY311 pKa = 10.1 ARR313 pKa = 11.84 QVQYY317 pKa = 11.09 GRR319 pKa = 11.84 KK320 pKa = 6.81 PTDD323 pKa = 3.28 MVPPLLSSVLMGVHH337 pKa = 7.02 PEE339 pKa = 3.93 LLRR342 pKa = 11.84 EE343 pKa = 4.18 KK344 pKa = 10.42 DD345 pKa = 3.61 PPLCQPLQPPLGSSSATGDD364 pKa = 3.23 KK365 pKa = 11.1 AGDD368 pKa = 3.55 VSAEE372 pKa = 3.9 RR373 pKa = 11.84 AAGRR377 pKa = 11.84 ASEE380 pKa = 4.53 AEE382 pKa = 4.01 GDD384 pKa = 3.78 RR385 pKa = 11.84 QYY387 pKa = 8.06 TTCGCLGACACGTLDD402 pKa = 3.46 TTEE405 pKa = 4.28 GEE407 pKa = 4.54 IVYY410 pKa = 9.79 HH411 pKa = 6.51 AARR414 pKa = 11.84 GTGPKK419 pKa = 9.48 PFCLGTGSGQIPCRR433 pKa = 11.84 GEE435 pKa = 3.94 DD436 pKa = 3.29 VRR438 pKa = 11.84 DD439 pKa = 3.77 ARR441 pKa = 11.84 DD442 pKa = 3.53 PTCQRR447 pKa = 11.84 EE448 pKa = 4.56 AIGEE452 pKa = 4.28 VTEE455 pKa = 5.53 GADD458 pKa = 3.97 QVDD461 pKa = 3.75 KK462 pKa = 8.99 TTTQRR467 pKa = 11.84 AASQFEE473 pKa = 4.27 PTPEE477 pKa = 3.66 DD478 pKa = 3.82 VIRR481 pKa = 11.84 LGARR485 pKa = 11.84 RR486 pKa = 11.84 KK487 pKa = 8.11 TSGIGTAAGDD497 pKa = 3.68 VLRR500 pKa = 11.84 PDD502 pKa = 3.55 IAQNQYY508 pKa = 10.69 PLLALDD514 pKa = 4.25 PRR516 pKa = 11.84 DD517 pKa = 3.6 LEE519 pKa = 4.3 SRR521 pKa = 11.84 RR522 pKa = 11.84 VQHH525 pKa = 5.78 VFSDD529 pKa = 3.72 EE530 pKa = 4.16 RR531 pKa = 11.84 AGSPEE536 pKa = 3.89 TGEE539 pKa = 4.28 PDD541 pKa = 3.11 QEE543 pKa = 3.93 PRR545 pKa = 11.84 KK546 pKa = 10.01 RR547 pKa = 11.84 KK548 pKa = 9.25 PEE550 pKa = 4.2 SGNGSPGASPFFKK563 pKa = 10.56 SIWGGGDD570 pKa = 3.39 YY571 pKa = 10.78 VRR573 pKa = 11.84 QSPPLGYY580 pKa = 10.82 GPTKK584 pKa = 10.63 DD585 pKa = 3.08 RR586 pKa = 11.84 AVGDD590 pKa = 3.97 TYY592 pKa = 11.26 WNYY595 pKa = 10.74 TGTDD599 pKa = 3.72 DD600 pKa = 4.19 EE601 pKa = 4.76 QEE603 pKa = 3.91 RR604 pKa = 11.84 CEE606 pKa = 4.05 ADD608 pKa = 3.37 GVGDD612 pKa = 3.52 EE613 pKa = 6.04 DD614 pKa = 4.73 EE615 pKa = 5.97 YY616 pKa = 11.5 EE617 pKa = 4.64 DD618 pKa = 5.86 CDD620 pKa = 4.38 EE621 pKa = 5.62 LDD623 pKa = 4.61 LRR625 pKa = 11.84 DD626 pKa = 3.88 TKK628 pKa = 10.87 TGGCHH633 pKa = 5.3 ATLGGDD639 pKa = 4.18 GNQEE643 pKa = 3.92 PPFLLLRR650 pKa = 11.84 DD651 pKa = 4.56 FEE653 pKa = 5.37 ALLKK657 pKa = 9.79 TGSSEE662 pKa = 3.82 GLSPHH667 pKa = 6.04 IQVSHH672 pKa = 5.23 GTEE675 pKa = 3.97 KK676 pKa = 10.09 EE677 pKa = 4.17 TLEE680 pKa = 5.59 DD681 pKa = 3.88 GGSCLKK687 pKa = 10.87 VYY689 pKa = 10.71 EE690 pKa = 5.01 DD691 pKa = 4.25 DD692 pKa = 4.48 SKK694 pKa = 11.86 GEE696 pKa = 4.1 SGGNPRR702 pKa = 11.84 DD703 pKa = 3.46 VTGNVEE709 pKa = 4.27 DD710 pKa = 5.35 AVDD713 pKa = 5.0 GYY715 pKa = 11.8 NSDD718 pKa = 4.6 DD719 pKa = 5.12 LGISCCQDD727 pKa = 2.37 GWSGDD732 pKa = 3.61 DD733 pKa = 4.01 GEE735 pKa = 5.58 AVDD738 pKa = 5.59 TEE740 pKa = 5.49 DD741 pKa = 3.99 EE742 pKa = 4.09 DD743 pKa = 4.79 TYY745 pKa = 11.51 TNQEE749 pKa = 3.83 GCGGGVEE756 pKa = 4.2 EE757 pKa = 5.97 GEE759 pKa = 3.96 DD760 pKa = 3.25 HH761 pKa = 7.78 GIGEE765 pKa = 4.48 EE766 pKa = 3.99 EE767 pKa = 4.7 DD768 pKa = 4.95 GGDD771 pKa = 3.19 EE772 pKa = 4.38 CYY774 pKa = 10.51 EE775 pKa = 4.23 DD776 pKa = 4.66 EE777 pKa = 4.8 GAQSSYY783 pKa = 10.07 YY784 pKa = 10.78 DD785 pKa = 3.62 RR786 pKa = 11.84 VAGDD790 pKa = 3.4 NADD793 pKa = 3.73 EE794 pKa = 4.68 LGEE797 pKa = 4.0 EE798 pKa = 4.23 LGEE801 pKa = 4.35 DD802 pKa = 3.84 DD803 pKa = 5.41 EE804 pKa = 6.09 LGEE807 pKa = 4.08 EE808 pKa = 4.4 LGDD811 pKa = 3.74 EE812 pKa = 4.25 EE813 pKa = 5.23 SVEE816 pKa = 5.1 DD817 pKa = 4.52 EE818 pKa = 4.63 DD819 pKa = 5.99 SGDD822 pKa = 3.73 GGDD825 pKa = 3.98 GEE827 pKa = 4.98 EE828 pKa = 4.02 VCEE831 pKa = 4.98 DD832 pKa = 4.06 YY833 pKa = 10.9 EE834 pKa = 4.52 VCDD837 pKa = 3.78 GDD839 pKa = 4.3 EE840 pKa = 4.12 EE841 pKa = 5.04 CEE843 pKa = 4.12 EE844 pKa = 4.52 GNDD847 pKa = 3.57 TGLEE851 pKa = 4.05 TLEE854 pKa = 5.18 DD855 pKa = 4.08 SVDD858 pKa = 3.68 EE859 pKa = 4.5 EE860 pKa = 4.29 NMGIYY865 pKa = 9.98 FRR867 pKa = 11.84 EE868 pKa = 4.59 HH869 pKa = 5.35 II870 pKa = 4.38
Molecular weight: 94.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.235
IPC2_protein 4.266
IPC_protein 4.253
Toseland 4.075
ProMoST 4.355
Dawson 4.202
Bjellqvist 4.342
Wikipedia 4.062
Rodwell 4.075
Grimsley 3.986
Solomon 4.202
Lehninger 4.151
Nozaki 4.304
DTASelect 4.469
Thurlkill 4.075
EMBOSS 4.088
Sillero 4.355
Patrickios 3.261
IPC_peptide 4.202
IPC2_peptide 4.342
IPC2.peptide.svr19 4.281
Protein with the highest isoelectric point:
>tr|A0A2D1A3K3|A0A2D1A3K3_9GAMA DNA polymerase processivity subunit OS=Eptesicus fuscus gammaherpesvirus OX=2035399 PE=4 SV=1
MM1 pKa = 7.11 YY2 pKa = 9.06 PRR4 pKa = 11.84 RR5 pKa = 11.84 LSRR8 pKa = 11.84 QRR10 pKa = 11.84 STLSVFRR17 pKa = 11.84 FRR19 pKa = 11.84 VTLIYY24 pKa = 10.51 CDD26 pKa = 3.79 VVDD29 pKa = 4.9 PSSDD33 pKa = 2.97 HH34 pKa = 6.93 LRR36 pKa = 11.84 FCISHH41 pKa = 6.95 RR42 pKa = 11.84 EE43 pKa = 3.92 LSWRR47 pKa = 11.84 SRR49 pKa = 11.84 YY50 pKa = 8.98 RR51 pKa = 11.84 ACSDD55 pKa = 2.55 SWKK58 pKa = 9.25 TLTCSSGIRR67 pKa = 11.84 CGCWPACLPPVANSVKK83 pKa = 9.95 RR84 pKa = 11.84 VSRR87 pKa = 11.84 STTQHH92 pKa = 5.06 ITTFTLDD99 pKa = 3.62 KK100 pKa = 10.15 GQHH103 pKa = 5.3 SQLIPQLTPQARR115 pKa = 11.84 CHH117 pKa = 6.38 LVLITWGDD125 pKa = 3.57 GGRR128 pKa = 11.84 ATASSARR135 pKa = 11.84 LPPTVIYY142 pKa = 8.66 CQPFAKK148 pKa = 10.06 RR149 pKa = 11.84 AFRR152 pKa = 11.84 HH153 pKa = 5.06 LCRR156 pKa = 11.84 MKK158 pKa = 10.88 LIIFTHH164 pKa = 5.79 MPFLIIILLFVLLQQ178 pKa = 3.59
Molecular weight: 20.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.589
IPC_protein 10.526
Toseland 10.54
ProMoST 10.35
Dawson 10.672
Bjellqvist 10.482
Wikipedia 10.921
Rodwell 10.745
Grimsley 10.73
Solomon 10.804
Lehninger 10.76
Nozaki 10.643
DTASelect 10.438
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.628
Patrickios 10.452
IPC_peptide 10.804
IPC2_peptide 10.028
IPC2.peptide.svr19 8.482
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
42028
89
3622
560.4
61.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.712 ± 0.269
2.184 ± 0.157
5.092 ± 0.186
5.703 ± 0.252
3.609 ± 0.188
7.321 ± 0.301
2.441 ± 0.093
3.086 ± 0.163
2.77 ± 0.139
9.648 ± 0.214
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.11 ± 0.102
2.936 ± 0.11
7.105 ± 0.294
3.89 ± 0.28
7.654 ± 0.242
7.371 ± 0.229
6.103 ± 0.307
6.489 ± 0.237
1.128 ± 0.076
2.646 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here