Human adenovirus E serotype 4 (HAdV-4) (Human adenovirus 4)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Mastadenovirus; Human mastadenovirus E

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5VH99|Q5VH99_ADE04 Isoform of Q5GF89 Control protein E4orf4 OS=Human adenovirus E serotype 4 OX=28280 GN=E4 PE=4 SV=1
MM1 pKa = 7.93LEE3 pKa = 4.35RR4 pKa = 11.84TPCTYY9 pKa = 10.69SIVVPEE15 pKa = 4.42ALNVHH20 pKa = 6.79LDD22 pKa = 3.5DD23 pKa = 4.6FSFVNFLKK31 pKa = 10.84NCLPDD36 pKa = 3.87FLSSYY41 pKa = 10.98LEE43 pKa = 5.26DD44 pKa = 3.2ITGSSQHH51 pKa = 6.73AYY53 pKa = 10.77FNLTFGNAHH62 pKa = 6.32WGGLRR67 pKa = 11.84FICNVACPSLVPGGPMAKK85 pKa = 10.15NFGEE89 pKa = 4.46DD90 pKa = 3.14MNEE93 pKa = 3.95YY94 pKa = 9.84IQLLLRR100 pKa = 11.84EE101 pKa = 4.35EE102 pKa = 4.7LRR104 pKa = 11.84DD105 pKa = 3.56CGRR108 pKa = 11.84AIDD111 pKa = 3.97IPLVNLLQVNQEE123 pKa = 3.99QNLLEE128 pKa = 4.19LL129 pKa = 4.61

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GFB2|Q5GFB2_ADE04 Isoform of Q5GFB3 Pre-histone-like nucleoprotein OS=Human adenovirus E serotype 4 OX=28280 GN=L2 PE=2 SV=1
MM1 pKa = 7.75ALTCRR6 pKa = 11.84IRR8 pKa = 11.84VPITGYY14 pKa = 10.06RR15 pKa = 11.84GRR17 pKa = 11.84KK18 pKa = 6.64PRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84LAGSGMRR30 pKa = 11.84RR31 pKa = 11.84HH32 pKa = 4.98PHH34 pKa = 5.04RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AISKK42 pKa = 9.94RR43 pKa = 11.84LGGGFLPALIPIIAAAIGAIPGIASVAVQASQRR76 pKa = 11.84HH77 pKa = 4.3

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

24

38

11653

50

1193

306.7

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.946 ± 0.335

1.871 ± 0.307

5.012 ± 0.295

6.153 ± 0.44

3.716 ± 0.232

6.239 ± 0.248

2.497 ± 0.207

3.93 ± 0.234

3.664 ± 0.319

9.586 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.218

4.291 ± 0.387

6.676 ± 0.268

4.497 ± 0.244

7.38 ± 0.585

6.814 ± 0.392

6.144 ± 0.417

6.359 ± 0.272

1.322 ± 0.102

3.261 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski