Hypericibacter adhaerens 
Average proteome isoelectric point is 6.83 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 5227 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A5J6N5H7|A0A5J6N5H7_9PROT Uncharacterized protein OS=Hypericibacter adhaerens OX=2602016 GN=FRZ61_51170 PE=4 SV=1MM1 pKa = 7.51  KK2 pKa = 10.41  NVLLGTTALVAAGLVASHH20 pKa = 6.95  AEE22 pKa = 3.87  AAQGVQLGIGGYY34 pKa = 8.36  YY35 pKa = 9.87  AAAAGLLFDD44 pKa = 4.7  QSDD47 pKa = 4.19  GNGDD51 pKa = 3.97  PGDD54 pKa = 3.73  HH55 pKa = 6.43  TRR57 pKa = 11.84  DD58 pKa = 2.91  IVFRR62 pKa = 11.84  QDD64 pKa = 3.1  VEE66 pKa = 4.0  IHH68 pKa = 6.19  FKK70 pKa = 11.55  GEE72 pKa = 3.95  TTLDD76 pKa = 3.17  NGLTVGARR84 pKa = 11.84  VEE86 pKa = 4.93  LEE88 pKa = 4.18  GQQSTDD94 pKa = 3.34  QIDD97 pKa = 3.88  EE98 pKa = 3.7  VWAYY102 pKa = 10.17  FKK104 pKa = 11.24  GGWGEE109 pKa = 4.17  LRR111 pKa = 11.84  FGDD114 pKa = 5.18  DD115 pKa = 5.06  DD116 pKa = 5.33  DD117 pKa = 4.43  ATEE120 pKa = 3.79  QLGYY124 pKa = 10.28  IIPSASNIFGVDD136 pKa = 3.03  TPYY139 pKa = 11.11  FEE141 pKa = 5.15  FANNHH146 pKa = 5.37  GGFTNGVFQTNSTLMDD162 pKa = 3.56  ISSDD166 pKa = 3.17  ATKK169 pKa = 10.11  IIYY172 pKa = 8.86  FSPTFAGFSFAASFAPDD189 pKa = 3.05  RR190 pKa = 11.84  RR191 pKa = 11.84  GEE193 pKa = 3.98  DD194 pKa = 3.33  TYY196 pKa = 11.46  SYY198 pKa = 10.42  WSGPGGTTNSNNTGQVQNVFAAALNFDD225 pKa = 3.77  HH226 pKa = 7.57  DD227 pKa = 4.57  FNGVSLATGVGFAEE241 pKa = 4.89  GQWEE245 pKa = 4.61  TKK247 pKa = 10.72  ASGANVDD254 pKa = 4.01  TTWGVNGHH262 pKa = 6.98  LDD264 pKa = 3.37  VSFSGFTIGGSGMYY278 pKa = 9.51  RR279 pKa = 11.84  DD280 pKa = 4.23  NWQSSAGTDD289 pKa = 3.27  YY290 pKa = 10.47  WVAGIGATYY299 pKa = 10.63  NWDD302 pKa = 2.79  AWTVGLAWSHH312 pKa = 6.14  GDD314 pKa = 3.7  YY315 pKa = 10.67  EE316 pKa = 4.57  YY317 pKa = 11.04  TPSNDD322 pKa = 3.46  DD323 pKa = 3.57  DD324 pKa = 6.17  KK325 pKa = 11.68  IDD327 pKa = 3.32  IVEE330 pKa = 4.08  FTGRR334 pKa = 11.84  YY335 pKa = 9.5  DD336 pKa = 3.99  LGPGISLDD344 pKa = 3.73  GMVGYY349 pKa = 11.24  NDD351 pKa = 3.79  MNAGDD356 pKa = 4.66  TGHH359 pKa = 6.67  QDD361 pKa = 3.42  DD362 pKa = 4.9  KK363 pKa = 10.76  TWEE366 pKa = 4.3  AGVGFYY372 pKa = 10.23  IGFF375 pKa = 3.99  
 40.15 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.745 
IPC2_protein 3.897 
IPC_protein 3.935 
Toseland    3.706 
ProMoST     4.101 
Dawson      3.935 
Bjellqvist  4.075 
Wikipedia   3.872 
Rodwell     3.757 
Grimsley    3.605 
Solomon     3.923 
Lehninger   3.884 
Nozaki      4.037 
DTASelect   4.317 
Thurlkill   3.757 
EMBOSS      3.884 
Sillero     4.05 
Patrickios  1.926 
IPC_peptide 3.923 
IPC2_peptide  4.037 
IPC2.peptide.svr19  3.938 
 Protein with the highest isoelectric point: 
>tr|A0A5J6MTN6|A0A5J6MTN6_9PROT Polyphosphate kinase OS=Hypericibacter adhaerens OX=2602016 GN=FRZ61_06080 PE=3 SV=1MM1 pKa = 7.35  KK2 pKa = 9.36  RR3 pKa = 11.84  TYY5 pKa = 10.1  QPSVLVRR12 pKa = 11.84  KK13 pKa = 9.31  RR14 pKa = 11.84  RR15 pKa = 11.84  HH16 pKa = 4.43  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MATVGGRR28 pKa = 11.84  KK29 pKa = 9.34  VIASRR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 9.73  GRR39 pKa = 11.84  KK40 pKa = 8.92  RR41 pKa = 11.84  LSAA44 pKa = 3.96  
 5.1 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.431 
IPC2_protein 11.125 
IPC_protein 12.501 
Toseland    12.661 
ProMoST     13.159 
Dawson      12.661 
Bjellqvist  12.661 
Wikipedia   13.144 
Rodwell     12.34 
Grimsley    12.705 
Solomon     13.159 
Lehninger   13.056 
Nozaki      12.661 
DTASelect   12.661 
Thurlkill   12.661 
EMBOSS      13.159 
Sillero     12.661 
Patrickios  12.076 
IPC_peptide 13.159 
IPC2_peptide  12.149 
IPC2.peptide.svr19  9.079 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        5227 
0
5227 
1721620
39
5653
329.4
35.71
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        12.745 ± 0.057
0.869 ± 0.01
5.508 ± 0.056
5.638 ± 0.034
3.556 ± 0.018
8.957 ± 0.061
2.073 ± 0.017
5.077 ± 0.025
3.217 ± 0.028
10.656 ± 0.052
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.319 ± 0.016
2.435 ± 0.029
5.515 ± 0.032
3.177 ± 0.02
7.385 ± 0.049
5.059 ± 0.027
5.042 ± 0.055
7.038 ± 0.03
1.436 ± 0.014
2.299 ± 0.019
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here