Hypericibacter adhaerens
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5227 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6N5H7|A0A5J6N5H7_9PROT Uncharacterized protein OS=Hypericibacter adhaerens OX=2602016 GN=FRZ61_51170 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.41 NVLLGTTALVAAGLVASHH20 pKa = 6.95 AEE22 pKa = 3.87 AAQGVQLGIGGYY34 pKa = 8.36 YY35 pKa = 9.87 AAAAGLLFDD44 pKa = 4.7 QSDD47 pKa = 4.19 GNGDD51 pKa = 3.97 PGDD54 pKa = 3.73 HH55 pKa = 6.43 TRR57 pKa = 11.84 DD58 pKa = 2.91 IVFRR62 pKa = 11.84 QDD64 pKa = 3.1 VEE66 pKa = 4.0 IHH68 pKa = 6.19 FKK70 pKa = 11.55 GEE72 pKa = 3.95 TTLDD76 pKa = 3.17 NGLTVGARR84 pKa = 11.84 VEE86 pKa = 4.93 LEE88 pKa = 4.18 GQQSTDD94 pKa = 3.34 QIDD97 pKa = 3.88 EE98 pKa = 3.7 VWAYY102 pKa = 10.17 FKK104 pKa = 11.24 GGWGEE109 pKa = 4.17 LRR111 pKa = 11.84 FGDD114 pKa = 5.18 DD115 pKa = 5.06 DD116 pKa = 5.33 DD117 pKa = 4.43 ATEE120 pKa = 3.79 QLGYY124 pKa = 10.28 IIPSASNIFGVDD136 pKa = 3.03 TPYY139 pKa = 11.11 FEE141 pKa = 5.15 FANNHH146 pKa = 5.37 GGFTNGVFQTNSTLMDD162 pKa = 3.56 ISSDD166 pKa = 3.17 ATKK169 pKa = 10.11 IIYY172 pKa = 8.86 FSPTFAGFSFAASFAPDD189 pKa = 3.05 RR190 pKa = 11.84 RR191 pKa = 11.84 GEE193 pKa = 3.98 DD194 pKa = 3.33 TYY196 pKa = 11.46 SYY198 pKa = 10.42 WSGPGGTTNSNNTGQVQNVFAAALNFDD225 pKa = 3.77 HH226 pKa = 7.57 DD227 pKa = 4.57 FNGVSLATGVGFAEE241 pKa = 4.89 GQWEE245 pKa = 4.61 TKK247 pKa = 10.72 ASGANVDD254 pKa = 4.01 TTWGVNGHH262 pKa = 6.98 LDD264 pKa = 3.37 VSFSGFTIGGSGMYY278 pKa = 9.51 RR279 pKa = 11.84 DD280 pKa = 4.23 NWQSSAGTDD289 pKa = 3.27 YY290 pKa = 10.47 WVAGIGATYY299 pKa = 10.63 NWDD302 pKa = 2.79 AWTVGLAWSHH312 pKa = 6.14 GDD314 pKa = 3.7 YY315 pKa = 10.67 EE316 pKa = 4.57 YY317 pKa = 11.04 TPSNDD322 pKa = 3.46 DD323 pKa = 3.57 DD324 pKa = 6.17 KK325 pKa = 11.68 IDD327 pKa = 3.32 IVEE330 pKa = 4.08 FTGRR334 pKa = 11.84 YY335 pKa = 9.5 DD336 pKa = 3.99 LGPGISLDD344 pKa = 3.73 GMVGYY349 pKa = 11.24 NDD351 pKa = 3.79 MNAGDD356 pKa = 4.66 TGHH359 pKa = 6.67 QDD361 pKa = 3.42 DD362 pKa = 4.9 KK363 pKa = 10.76 TWEE366 pKa = 4.3 AGVGFYY372 pKa = 10.23 IGFF375 pKa = 3.99
Molecular weight: 40.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.897
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.757
Grimsley 3.605
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.317
Thurlkill 3.757
EMBOSS 3.884
Sillero 4.05
Patrickios 1.926
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.938
Protein with the highest isoelectric point:
>tr|A0A5J6MTN6|A0A5J6MTN6_9PROT Polyphosphate kinase OS=Hypericibacter adhaerens OX=2602016 GN=FRZ61_06080 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.1 QPSVLVRR12 pKa = 11.84 KK13 pKa = 9.31 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.43 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.34 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5227
0
5227
1721620
39
5653
329.4
35.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.745 ± 0.057
0.869 ± 0.01
5.508 ± 0.056
5.638 ± 0.034
3.556 ± 0.018
8.957 ± 0.061
2.073 ± 0.017
5.077 ± 0.025
3.217 ± 0.028
10.656 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.319 ± 0.016
2.435 ± 0.029
5.515 ± 0.032
3.177 ± 0.02
7.385 ± 0.049
5.059 ± 0.027
5.042 ± 0.055
7.038 ± 0.03
1.436 ± 0.014
2.299 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here