Aquifex aeolicus (strain VF5)

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Aquifex; Aquifex aeolicus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O66658|O66658_AQUAE HTH tetR-type domain-containing protein OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_321 PE=4 SV=1
MM1 pKa = 7.5SVILEE6 pKa = 3.91FLATGFLVGKK16 pKa = 10.03LPVAPGTFGTLVGIPLVLLLYY37 pKa = 10.47LDD39 pKa = 4.05RR40 pKa = 11.84NLYY43 pKa = 10.8LFGTLIFFFLGWISSEE59 pKa = 3.98YY60 pKa = 9.41MVQNLRR66 pKa = 11.84DD67 pKa = 3.87EE68 pKa = 5.45DD69 pKa = 4.21PEE71 pKa = 4.11QVVIDD76 pKa = 4.74EE77 pKa = 4.24IVGYY81 pKa = 7.91LLCFLFVEE89 pKa = 4.77PTLKK93 pKa = 10.74SLILAFIIFRR103 pKa = 11.84VVDD106 pKa = 3.41ILKK109 pKa = 9.45PFPVNLFEE117 pKa = 4.72NFPGGLGVMMDD128 pKa = 4.13DD129 pKa = 3.86VVGGLITSVVLYY141 pKa = 10.88LLLQQ145 pKa = 3.79

Molecular weight: 16.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O66575|Y194_AQUAE Uncharacterized protein aq_194 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_194 PE=4 SV=1
MM1 pKa = 6.4VTEE4 pKa = 4.24GLKK7 pKa = 10.16PHH9 pKa = 7.07ISIKK13 pKa = 9.73KK14 pKa = 8.7KK15 pKa = 9.39KK16 pKa = 9.43RR17 pKa = 11.84KK18 pKa = 9.7SGFLARR24 pKa = 11.84MRR26 pKa = 11.84TKK28 pKa = 10.37SGRR31 pKa = 11.84KK32 pKa = 8.22IIARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.1GRR42 pKa = 11.84KK43 pKa = 8.79RR44 pKa = 11.84LAPP47 pKa = 3.99

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1553

0

1553

488987

37

1574

314.9

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.876 ± 0.066

0.793 ± 0.022

4.32 ± 0.037

9.647 ± 0.091

5.154 ± 0.069

6.73 ± 0.051

1.546 ± 0.025

7.328 ± 0.047

9.441 ± 0.069

10.58 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.928 ± 0.023

3.614 ± 0.04

4.062 ± 0.037

2.049 ± 0.025

4.917 ± 0.044

4.807 ± 0.041

4.213 ± 0.035

7.912 ± 0.057

0.938 ± 0.021

4.144 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski