Amino acid dipepetide frequency for Aquifex aeolicus (strain VF5)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.517AlaAla: 2.517 ± 0.108
0.646AlaCys: 0.646 ± 0.043
2.372AlaAsp: 2.372 ± 0.071
4.131AlaGlu: 4.131 ± 0.091
3.215AlaPhe: 3.215 ± 0.09
4.401AlaGly: 4.401 ± 0.114
1.065AlaHis: 1.065 ± 0.045
4.483AlaIle: 4.483 ± 0.087
5.563AlaLys: 5.563 ± 0.121
7.354AlaLeu: 7.354 ± 0.144
1.284AlaMet: 1.284 ± 0.053
1.822AlaAsn: 1.822 ± 0.06
1.875AlaPro: 1.875 ± 0.062
1.867AlaGln: 1.867 ± 0.063
2.718AlaArg: 2.718 ± 0.082
2.847AlaSer: 2.847 ± 0.079
2.342AlaThr: 2.342 ± 0.076
4.963AlaVal: 4.963 ± 0.112
0.505AlaTrp: 0.505 ± 0.039
2.789AlaTyr: 2.789 ± 0.075
0.0AlaXaa: 0.0 ± 0.0
Cys
0.503CysAla: 0.503 ± 0.03
0.115CysCys: 0.115 ± 0.016
0.327CysAsp: 0.327 ± 0.031
0.675CysGlu: 0.675 ± 0.04
0.313CysPhe: 0.313 ± 0.027
0.838CysGly: 0.838 ± 0.052
0.202CysHis: 0.202 ± 0.024
0.476CysIle: 0.476 ± 0.032
0.54CysLys: 0.54 ± 0.037
0.603CysLeu: 0.603 ± 0.034
0.194CysMet: 0.194 ± 0.023
0.274CysAsn: 0.274 ± 0.025
0.556CysPro: 0.556 ± 0.035
0.139CysGln: 0.139 ± 0.017
0.372CysArg: 0.372 ± 0.026
0.511CysSer: 0.511 ± 0.034
0.364CysThr: 0.364 ± 0.029
0.562CysVal: 0.562 ± 0.036
0.051CysTrp: 0.051 ± 0.009
0.313CysTyr: 0.313 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
2.507AspAla: 2.507 ± 0.073
0.378AspCys: 0.378 ± 0.029
1.268AspAsp: 1.268 ± 0.063
3.657AspGlu: 3.657 ± 0.085
2.947AspPhe: 2.947 ± 0.079
2.432AspGly: 2.432 ± 0.077
0.444AspHis: 0.444 ± 0.031
3.888AspIle: 3.888 ± 0.099
3.583AspLys: 3.583 ± 0.086
4.775AspLeu: 4.775 ± 0.096
0.961AspMet: 0.961 ± 0.052
1.182AspAsn: 1.182 ± 0.05
2.061AspPro: 2.061 ± 0.069
0.611AspGln: 0.611 ± 0.037
2.158AspArg: 2.158 ± 0.071
1.44AspSer: 1.44 ± 0.049
2.059AspThr: 2.059 ± 0.072
3.787AspVal: 3.787 ± 0.098
0.583AspTrp: 0.583 ± 0.039
2.475AspTyr: 2.475 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.333GluAla: 5.333 ± 0.126
0.552GluCys: 0.552 ± 0.036
4.311GluAsp: 4.311 ± 0.091
12.219GluGlu: 12.219 ± 0.249
4.495GluPhe: 4.495 ± 0.101
6.27GluGly: 6.27 ± 0.127
1.352GluHis: 1.352 ± 0.051
7.552GluIle: 7.552 ± 0.122
10.784GluLys: 10.784 ± 0.179
9.884GluLeu: 9.884 ± 0.192
1.638GluMet: 1.638 ± 0.06
4.078GluAsn: 4.078 ± 0.086
2.401GluPro: 2.401 ± 0.076
1.558GluGln: 1.558 ± 0.059
5.383GluArg: 5.383 ± 0.115
3.063GluSer: 3.063 ± 0.082
3.231GluThr: 3.231 ± 0.086
8.44GluVal: 8.44 ± 0.146
0.836GluTrp: 0.836 ± 0.043
3.088GluTyr: 3.088 ± 0.08
0.0GluXaa: 0.0 ± 0.0
Phe
2.72PheAla: 2.72 ± 0.08
0.429PheCys: 0.429 ± 0.031
1.998PheAsp: 1.998 ± 0.068
3.839PheGlu: 3.839 ± 0.09
3.088PhePhe: 3.088 ± 0.102
3.491PheGly: 3.491 ± 0.078
0.761PheHis: 0.761 ± 0.036
3.747PheIle: 3.747 ± 0.096
4.225PheLys: 4.225 ± 0.102
6.773PheLeu: 6.773 ± 0.177
1.008PheMet: 1.008 ± 0.044
1.687PheAsn: 1.687 ± 0.054
2.149PhePro: 2.149 ± 0.072
1.047PheGln: 1.047 ± 0.046
2.164PheArg: 2.164 ± 0.068
3.411PheSer: 3.411 ± 0.103
2.423PheThr: 2.423 ± 0.079
3.693PheVal: 3.693 ± 0.094
0.538PheTrp: 0.538 ± 0.031
2.346PheTyr: 2.346 ± 0.077
0.0PheXaa: 0.0 ± 0.0
Gly
4.109GlyAla: 4.109 ± 0.101
0.632GlyCys: 0.632 ± 0.04
3.104GlyAsp: 3.104 ± 0.076
5.624GlyGlu: 5.624 ± 0.109
3.74GlyPhe: 3.74 ± 0.088
4.648GlyGly: 4.648 ± 0.129
1.151GlyHis: 1.151 ± 0.055
6.017GlyIle: 6.017 ± 0.118
6.871GlyLys: 6.871 ± 0.122
5.894GlyLeu: 5.894 ± 0.103
1.489GlyMet: 1.489 ± 0.06
2.515GlyAsn: 2.515 ± 0.081
1.221GlyPro: 1.221 ± 0.058
1.239GlyGln: 1.239 ± 0.054
3.305GlyArg: 3.305 ± 0.087
3.196GlySer: 3.196 ± 0.09
3.188GlyThr: 3.188 ± 0.08
5.695GlyVal: 5.695 ± 0.11
0.722GlyTrp: 0.722 ± 0.039
2.937GlyTyr: 2.937 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
0.945HisAla: 0.945 ± 0.048
0.172HisCys: 0.172 ± 0.021
0.552HisAsp: 0.552 ± 0.034
1.125HisGlu: 1.125 ± 0.044
0.939HisPhe: 0.939 ± 0.049
1.168HisGly: 1.168 ± 0.057
0.374HisHis: 0.374 ± 0.035
1.115HisIle: 1.115 ± 0.051
0.912HisLys: 0.912 ± 0.042
1.624HisLeu: 1.624 ± 0.064
0.376HisMet: 0.376 ± 0.028
0.491HisAsn: 0.491 ± 0.035
1.035HisPro: 1.035 ± 0.042
0.317HisGln: 0.317 ± 0.027
0.841HisArg: 0.841 ± 0.043
0.734HisSer: 0.734 ± 0.039
0.765HisThr: 0.765 ± 0.04
1.049HisVal: 1.049 ± 0.046
0.176HisTrp: 0.176 ± 0.019
0.753HisTyr: 0.753 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
4.792IleAla: 4.792 ± 0.107
0.546IleCys: 0.546 ± 0.036
3.423IleAsp: 3.423 ± 0.104
5.931IleGlu: 5.931 ± 0.129
3.397IlePhe: 3.397 ± 0.088
4.589IleGly: 4.589 ± 0.107
1.157IleHis: 1.157 ± 0.043
4.62IleIle: 4.62 ± 0.12
6.947IleLys: 6.947 ± 0.119
8.168IleLeu: 8.168 ± 0.15
1.25IleMet: 1.25 ± 0.049
2.947IleAsn: 2.947 ± 0.08
4.088IlePro: 4.088 ± 0.093
1.695IleGln: 1.695 ± 0.057
3.423IleArg: 3.423 ± 0.096
4.213IleSer: 4.213 ± 0.105
3.775IleThr: 3.775 ± 0.095
5.051IleVal: 5.051 ± 0.106
0.452IleTrp: 0.452 ± 0.033
2.818IleTyr: 2.818 ± 0.085
0.0IleXaa: 0.0 ± 0.0
Lys
5.695LysAla: 5.695 ± 0.126
0.458LysCys: 0.458 ± 0.031
4.499LysAsp: 4.499 ± 0.101
12.806LysGlu: 12.806 ± 0.218
3.628LysPhe: 3.628 ± 0.08
6.765LysGly: 6.765 ± 0.148
1.325LysHis: 1.325 ± 0.054
6.372LysIle: 6.372 ± 0.12
9.25LysLys: 9.25 ± 0.162
8.937LysLeu: 8.937 ± 0.139
1.466LysMet: 1.466 ± 0.055
3.837LysAsn: 3.837 ± 0.09
3.489LysPro: 3.489 ± 0.087
1.421LysGln: 1.421 ± 0.052
4.865LysArg: 4.865 ± 0.113
3.425LysSer: 3.425 ± 0.082
3.671LysThr: 3.671 ± 0.095
8.432LysVal: 8.432 ± 0.141
0.761LysTrp: 0.761 ± 0.043
3.311LysTyr: 3.311 ± 0.082
0.0LysXaa: 0.0 ± 0.0
Leu
6.254LeuAla: 6.254 ± 0.124
0.685LeuCys: 0.685 ± 0.039
4.139LeuAsp: 4.139 ± 0.099
9.577LeuGlu: 9.577 ± 0.165
5.082LeuPhe: 5.082 ± 0.142
7.381LeuGly: 7.381 ± 0.122
1.499LeuHis: 1.499 ± 0.053
7.898LeuIle: 7.898 ± 0.148
11.882LeuLys: 11.882 ± 0.201
10.563LeuLeu: 10.563 ± 0.21
2.205LeuMet: 2.205 ± 0.068
3.853LeuAsn: 3.853 ± 0.106
4.323LeuPro: 4.323 ± 0.092
2.153LeuGln: 2.153 ± 0.067
5.244LeuArg: 5.244 ± 0.111
6.947LeuSer: 6.947 ± 0.155
4.409LeuThr: 4.409 ± 0.095
6.593LeuVal: 6.593 ± 0.111
1.037LeuTrp: 1.037 ± 0.051
4.074LeuTyr: 4.074 ± 0.098
0.0LeuXaa: 0.0 ± 0.0
Met
1.356MetAla: 1.356 ± 0.06
0.143MetCys: 0.143 ± 0.018
0.957MetAsp: 0.957 ± 0.046
1.963MetGlu: 1.963 ± 0.067
0.845MetPhe: 0.845 ± 0.04
1.464MetGly: 1.464 ± 0.069
0.342MetHis: 0.342 ± 0.026
1.127MetIle: 1.127 ± 0.048
2.035MetLys: 2.035 ± 0.07
1.736MetLeu: 1.736 ± 0.06
0.372MetMet: 0.372 ± 0.03
0.789MetAsn: 0.789 ± 0.039
0.843MetPro: 0.843 ± 0.043
0.342MetGln: 0.342 ± 0.029
1.096MetArg: 1.096 ± 0.047
0.996MetSer: 0.996 ± 0.046
0.753MetThr: 0.753 ± 0.045
1.321MetVal: 1.321 ± 0.049
0.205MetTrp: 0.205 ± 0.018
0.601MetTyr: 0.601 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
2.168AsnAla: 2.168 ± 0.062
0.376AsnCys: 0.376 ± 0.032
1.074AsnAsp: 1.074 ± 0.049
2.548AsnGlu: 2.548 ± 0.079
2.333AsnPhe: 2.333 ± 0.071
1.976AsnGly: 1.976 ± 0.068
0.44AsnHis: 0.44 ± 0.03
2.358AsnIle: 2.358 ± 0.076
2.573AsnLys: 2.573 ± 0.071
4.998AsnLeu: 4.998 ± 0.108
0.667AsnMet: 0.667 ± 0.033
0.957AsnAsn: 0.957 ± 0.047
2.683AsnPro: 2.683 ± 0.08
0.72AsnGln: 0.72 ± 0.045
1.634AsnArg: 1.634 ± 0.057
1.501AsnSer: 1.501 ± 0.051
1.759AsnThr: 1.759 ± 0.062
3.176AsnVal: 3.176 ± 0.074
0.423AsnTrp: 0.423 ± 0.031
1.777AsnTyr: 1.777 ± 0.061
0.0AsnXaa: 0.0 ± 0.0
Pro
2.0ProAla: 2.0 ± 0.075
0.309ProCys: 0.309 ± 0.026
1.982ProAsp: 1.982 ± 0.063
4.933ProGlu: 4.933 ± 0.118
2.217ProPhe: 2.217 ± 0.073
2.102ProGly: 2.102 ± 0.074
0.798ProHis: 0.798 ± 0.038
2.468ProIle: 2.468 ± 0.074
3.423ProLys: 3.423 ± 0.081
3.865ProLeu: 3.865 ± 0.102
0.718ProMet: 0.718 ± 0.041
1.415ProAsn: 1.415 ± 0.061
1.879ProPro: 1.879 ± 0.07
1.133ProGln: 1.133 ± 0.056
1.548ProArg: 1.548 ± 0.054
2.098ProSer: 2.098 ± 0.068
1.82ProThr: 1.82 ± 0.068
3.503ProVal: 3.503 ± 0.086
0.391ProTrp: 0.391 ± 0.026
2.021ProTyr: 2.021 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
1.284GlnAla: 1.284 ± 0.05
0.143GlnCys: 0.143 ± 0.017
0.843GlnAsp: 0.843 ± 0.04
2.196GlnGlu: 2.196 ± 0.076
0.81GlnPhe: 0.81 ± 0.045
1.354GlnGly: 1.354 ± 0.049
0.276GlnHis: 0.276 ± 0.025
1.671GlnIle: 1.671 ± 0.065
2.219GlnLys: 2.219 ± 0.083
1.677GlnLeu: 1.677 ± 0.059
0.436GlnMet: 0.436 ± 0.032
0.996GlnAsn: 0.996 ± 0.042
0.671GlnPro: 0.671 ± 0.038
0.44GlnGln: 0.44 ± 0.035
1.272GlnArg: 1.272 ± 0.054
0.777GlnSer: 0.777 ± 0.035
1.023GlnThr: 1.023 ± 0.047
1.638GlnVal: 1.638 ± 0.064
0.164GlnTrp: 0.164 ± 0.019
0.599GlnTyr: 0.599 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
3.043ArgAla: 3.043 ± 0.082
0.309ArgCys: 0.309 ± 0.027
2.407ArgAsp: 2.407 ± 0.071
6.09ArgGlu: 6.09 ± 0.153
2.274ArgPhe: 2.274 ± 0.074
3.055ArgGly: 3.055 ± 0.082
0.667ArgHis: 0.667 ± 0.041
4.076ArgIle: 4.076 ± 0.089
4.781ArgLys: 4.781 ± 0.106
4.509ArgLeu: 4.509 ± 0.099
0.982ArgMet: 0.982 ± 0.046
1.853ArgAsn: 1.853 ± 0.059
1.458ArgPro: 1.458 ± 0.059
0.975ArgGln: 0.975 ± 0.049
2.683ArgArg: 2.683 ± 0.089
1.808ArgSer: 1.808 ± 0.068
1.914ArgThr: 1.914 ± 0.069
3.922ArgVal: 3.922 ± 0.087
0.517ArgTrp: 0.517 ± 0.035
1.843ArgTyr: 1.843 ± 0.062
0.002ArgXaa: 0.002 ± 0.002
Ser
2.869SerAla: 2.869 ± 0.079
0.479SerCys: 0.479 ± 0.033
1.902SerAsp: 1.902 ± 0.061
3.967SerGlu: 3.967 ± 0.089
3.014SerPhe: 3.014 ± 0.092
3.37SerGly: 3.37 ± 0.089
0.785SerHis: 0.785 ± 0.038
3.17SerIle: 3.17 ± 0.098
3.565SerLys: 3.565 ± 0.093
5.969SerLeu: 5.969 ± 0.123
1.031SerMet: 1.031 ± 0.051
1.354SerAsn: 1.354 ± 0.049
2.258SerPro: 2.258 ± 0.068
1.219SerGln: 1.219 ± 0.054
2.088SerArg: 2.088 ± 0.059
2.634SerSer: 2.634 ± 0.092
2.092SerThr: 2.092 ± 0.063
3.877SerVal: 3.877 ± 0.086
0.429SerTrp: 0.429 ± 0.029
1.998SerTyr: 1.998 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
2.789ThrAla: 2.789 ± 0.082
0.378ThrCys: 0.378 ± 0.031
1.881ThrAsp: 1.881 ± 0.069
2.937ThrGlu: 2.937 ± 0.075
2.534ThrPhe: 2.534 ± 0.072
3.403ThrGly: 3.403 ± 0.099
0.793ThrHis: 0.793 ± 0.04
2.495ThrIle: 2.495 ± 0.078
3.055ThrLys: 3.055 ± 0.084
5.264ThrLeu: 5.264 ± 0.113
0.787ThrMet: 0.787 ± 0.044
1.325ThrAsn: 1.325 ± 0.053
2.434ThrPro: 2.434 ± 0.072
1.018ThrGln: 1.018 ± 0.044
1.538ThrArg: 1.538 ± 0.059
1.967ThrSer: 1.967 ± 0.062
2.051ThrThr: 2.051 ± 0.066
3.26ThrVal: 3.26 ± 0.09
0.35ThrTrp: 0.35 ± 0.033
1.869ThrTyr: 1.869 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.712ValAla: 4.712 ± 0.105
0.718ValCys: 0.718 ± 0.042
3.62ValAsp: 3.62 ± 0.076
7.072ValGlu: 7.072 ± 0.137
4.098ValPhe: 4.098 ± 0.095
5.061ValGly: 5.061 ± 0.097
1.194ValHis: 1.194 ± 0.057
6.468ValIle: 6.468 ± 0.126
8.076ValLys: 8.076 ± 0.153
8.023ValLeu: 8.023 ± 0.142
1.515ValMet: 1.515 ± 0.06
3.086ValAsn: 3.086 ± 0.075
3.129ValPro: 3.129 ± 0.082
1.538ValGln: 1.538 ± 0.061
3.951ValArg: 3.951 ± 0.097
4.035ValSer: 4.035 ± 0.1
2.143ValThr: 2.143 ± 0.069
6.415ValVal: 6.415 ± 0.142
0.703ValTrp: 0.703 ± 0.045
3.567ValTyr: 3.567 ± 0.08
0.0ValXaa: 0.0 ± 0.0
Trp
0.593TrpAla: 0.593 ± 0.04
0.086TrpCys: 0.086 ± 0.014
0.534TrpAsp: 0.534 ± 0.033
1.104TrpGlu: 1.104 ± 0.055
0.44TrpPhe: 0.44 ± 0.031
0.636TrpGly: 0.636 ± 0.036
0.162TrpHis: 0.162 ± 0.018
0.703TrpIle: 0.703 ± 0.046
1.014TrpLys: 1.014 ± 0.046
0.793TrpLeu: 0.793 ± 0.042
0.176TrpMet: 0.176 ± 0.019
0.387TrpAsn: 0.387 ± 0.032
0.164TrpPro: 0.164 ± 0.018
0.221TrpGln: 0.221 ± 0.02
0.489TrpArg: 0.489 ± 0.03
0.384TrpSer: 0.384 ± 0.033
0.323TrpThr: 0.323 ± 0.026
0.74TrpVal: 0.74 ± 0.039
0.123TrpTrp: 0.123 ± 0.015
0.307TrpTyr: 0.307 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.571TyrAla: 2.571 ± 0.081
0.376TyrCys: 0.376 ± 0.026
2.002TyrAsp: 2.002 ± 0.061
4.076TyrGlu: 4.076 ± 0.103
2.129TyrPhe: 2.129 ± 0.075
2.894TyrGly: 2.894 ± 0.071
0.673TyrHis: 0.673 ± 0.036
2.579TyrIle: 2.579 ± 0.079
3.117TyrLys: 3.117 ± 0.088
4.387TyrLeu: 4.387 ± 0.095
0.728TyrMet: 0.728 ± 0.036
1.292TyrAsn: 1.292 ± 0.049
1.906TyrPro: 1.906 ± 0.062
0.832TyrGln: 0.832 ± 0.04
2.397TyrArg: 2.397 ± 0.08
2.084TyrSer: 2.084 ± 0.06
2.025TyrThr: 2.025 ± 0.066
3.004TyrVal: 3.004 ± 0.087
0.413TyrTrp: 0.413 ± 0.028
1.961TyrTyr: 1.961 ± 0.084
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.002XaaArg: 0.002 ± 0.002
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1553 proteins (488987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski