Erysipelatoclostridium ramosum DSM 1402

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelatoclostridium; Erysipelatoclostridium ramosum

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0N0N1|B0N0N1_9FIRM PhoH family protein OS=Erysipelatoclostridium ramosum DSM 1402 OX=445974 GN=CLORAM_00126 PE=4 SV=1
MM1 pKa = 7.45FSKK4 pKa = 10.91LYY6 pKa = 9.01ITIKK10 pKa = 10.8ASDD13 pKa = 4.13KK14 pKa = 10.86FDD16 pKa = 4.68DD17 pKa = 5.15SIIDD21 pKa = 4.35PISFILIDD29 pKa = 3.51WGVDD33 pKa = 3.06GNLIDD38 pKa = 3.57WDD40 pKa = 4.13IYY42 pKa = 9.82PSRR45 pKa = 11.84AKK47 pKa = 10.99LEE49 pKa = 4.26INTEE53 pKa = 3.66NDD55 pKa = 2.67NYY57 pKa = 8.98QTYY60 pKa = 10.34SITYY64 pKa = 9.84QGMQTIMEE72 pKa = 4.74LCYY75 pKa = 10.24EE76 pKa = 4.62CEE78 pKa = 3.88FVMTIIDD85 pKa = 3.73NDD87 pKa = 3.98EE88 pKa = 4.09KK89 pKa = 11.19SEE91 pKa = 4.28SYY93 pKa = 7.75TTYY96 pKa = 10.71FSRR99 pKa = 11.84EE100 pKa = 3.49NSNNFEE106 pKa = 4.15TDD108 pKa = 2.57ISEE111 pKa = 4.37LLEE114 pKa = 4.14

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0N292|B0N292_9FIRM Permease IIC component OS=Erysipelatoclostridium ramosum DSM 1402 OX=445974 GN=CLORAM_00728 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.02RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3166

0

3166

959423

39

4095

303.0

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.133 ± 0.047

1.329 ± 0.019

5.927 ± 0.042

6.588 ± 0.047

4.4 ± 0.035

6.2 ± 0.045

1.813 ± 0.021

9.242 ± 0.053

7.753 ± 0.037

9.46 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.024

5.887 ± 0.037

2.838 ± 0.023

3.334 ± 0.024

3.273 ± 0.03

5.753 ± 0.033

5.262 ± 0.043

6.552 ± 0.038

0.723 ± 0.012

4.713 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski