Hydrocoleum sp. CS-953

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Hydrocoleum; unclassified Hydrocoleum

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261KVW2|A0A261KVW2_9CYAN Uncharacterized protein OS=Hydrocoleum sp. CS-953 OX=1671698 GN=AFK68_05825 PE=4 SV=1
MM1 pKa = 7.34YY2 pKa = 10.53NGGLGVTNSGEE13 pKa = 4.41DD14 pKa = 3.93GSHH17 pKa = 6.03HH18 pKa = 6.63RR19 pKa = 11.84VDD21 pKa = 3.9NGTQVDD27 pKa = 4.03YY28 pKa = 11.6LLFEE32 pKa = 4.93FDD34 pKa = 3.92QDD36 pKa = 3.9VTVDD40 pKa = 3.56KK41 pKa = 11.46AFLEE45 pKa = 4.59YY46 pKa = 10.44VGHH49 pKa = 7.53DD50 pKa = 3.39SDD52 pKa = 3.64ISIWIGDD59 pKa = 3.86SNGAEE64 pKa = 3.88ISSLSDD70 pKa = 3.19SLLSSYY76 pKa = 10.88VKK78 pKa = 10.42EE79 pKa = 4.19NNFTHH84 pKa = 6.55SSSSRR89 pKa = 11.84WADD92 pKa = 3.36FNSANLTGDD101 pKa = 3.49TVIISLSAAGEE112 pKa = 4.17TSIGNYY118 pKa = 9.48VNIATVTAGSVSDD131 pKa = 4.36EE132 pKa = 4.28DD133 pKa = 3.55QSGYY137 pKa = 9.19TNPEE141 pKa = 4.01GEE143 pKa = 4.89PEE145 pKa = 4.26PEE147 pKa = 4.02PQQ149 pKa = 3.87

Molecular weight: 15.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261KNB2|A0A261KNB2_9CYAN Uncharacterized protein OS=Hydrocoleum sp. CS-953 OX=1671698 GN=AFK68_23835 PE=4 SV=1
MM1 pKa = 7.23FVGIPNPNQFWTPWVTVGIYY21 pKa = 9.41TPIRR25 pKa = 11.84LRR27 pKa = 11.84CQEE30 pKa = 4.29LAPRR34 pKa = 11.84IYY36 pKa = 9.57STQKK40 pKa = 9.88ILLTXVEE47 pKa = 5.26KK48 pKa = 10.51ILKK51 pKa = 9.52FRR53 pKa = 11.84IEE55 pKa = 3.96EE56 pKa = 4.0GVRR59 pKa = 11.84RR60 pKa = 11.84QKK62 pKa = 10.25FWRR65 pKa = 11.84QNRR68 pKa = 11.84CSWGFQTPTNSGHH81 pKa = 6.59HH82 pKa = 5.63GG83 pKa = 3.36

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4827

0

4827

1336359

30

3641

276.9

31.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.966 ± 0.038

0.998 ± 0.013

4.956 ± 0.034

6.926 ± 0.045

4.145 ± 0.029

6.677 ± 0.05

1.657 ± 0.02

7.445 ± 0.039

5.837 ± 0.041

10.498 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.018

5.145 ± 0.037

4.402 ± 0.032

4.862 ± 0.034

4.474 ± 0.028

6.514 ± 0.04

5.563 ± 0.038

6.342 ± 0.034

1.399 ± 0.016

3.225 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski