Amino acid dipepetide frequency for Hydrocoleum sp. CS-953

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.477AlaAla: 5.477 ± 0.078	0.719AlaCys: 0.719 ± 0.025	3.583AlaAsp: 3.583 ± 0.062	5.122AlaGlu: 5.122 ± 0.071	2.636AlaPhe: 2.636 ± 0.053	4.709AlaGly: 4.709 ± 0.071	1.089AlaHis: 1.089 ± 0.029	6.169AlaIle: 6.169 ± 0.082	4.327AlaLys: 4.327 ± 0.065	6.818AlaLeu: 6.818 ± 0.097	1.452AlaMet: 1.452 ± 0.034	3.582AlaAsn: 3.582 ± 0.068	2.185AlaPro: 2.185 ± 0.046	3.044AlaGln: 3.044 ± 0.063	2.82AlaArg: 2.82 ± 0.054	3.956AlaSer: 3.956 ± 0.062	4.224AlaThr: 4.224 ± 0.076	4.808AlaVal: 4.808 ± 0.067	0.823AlaTrp: 0.823 ± 0.029	2.083AlaTyr: 2.083 ± 0.046	0.034AlaXaa: 0.034 ± 0.006
Cys	0.53CysAla: 0.53 ± 0.022	0.178CysCys: 0.178 ± 0.012	0.465CysAsp: 0.465 ± 0.021	0.537CysGlu: 0.537 ± 0.023	0.449CysPhe: 0.449 ± 0.021	0.839CysGly: 0.839 ± 0.029	0.283CysHis: 0.283 ± 0.017	0.626CysIle: 0.626 ± 0.025	0.373CysLys: 0.373 ± 0.017	1.166CysLeu: 1.166 ± 0.034	0.169CysMet: 0.169 ± 0.012	0.433CysAsn: 0.433 ± 0.02	0.531CysPro: 0.531 ± 0.022	0.635CysGln: 0.635 ± 0.022	0.528CysArg: 0.528 ± 0.02	0.68CysSer: 0.68 ± 0.025	0.48CysThr: 0.48 ± 0.021	0.531CysVal: 0.531 ± 0.024	0.121CysTrp: 0.121 ± 0.009	0.427CysTyr: 0.427 ± 0.021	0.003CysXaa: 0.003 ± 0.002
Asp	3.175AspAla: 3.175 ± 0.058	0.497AspCys: 0.497 ± 0.018	2.471AspAsp: 2.471 ± 0.068	3.188AspGlu: 3.188 ± 0.054	2.567AspPhe: 2.567 ± 0.043	3.602AspGly: 3.602 ± 0.087	0.608AspHis: 0.608 ± 0.027	4.018AspIle: 4.018 ± 0.074	2.737AspLys: 2.737 ± 0.055	5.49AspLeu: 5.49 ± 0.076	0.802AspMet: 0.802 ± 0.027	2.523AspAsn: 2.523 ± 0.049	2.202AspPro: 2.202 ± 0.041	1.477AspGln: 1.477 ± 0.036	2.708AspArg: 2.708 ± 0.045	3.115AspSer: 3.115 ± 0.056	2.55AspThr: 2.55 ± 0.07	3.039AspVal: 3.039 ± 0.057	0.844AspTrp: 0.844 ± 0.025	1.926AspTyr: 1.926 ± 0.042	0.021AspXaa: 0.021 ± 0.004
Glu	5.273GluAla: 5.273 ± 0.08	0.486GluCys: 0.486 ± 0.018	2.927GluAsp: 2.927 ± 0.059	4.981GluGlu: 4.981 ± 0.084	2.689GluPhe: 2.689 ± 0.047	3.615GluGly: 3.615 ± 0.063	0.917GluHis: 0.917 ± 0.027	5.934GluIle: 5.934 ± 0.063	5.391GluLys: 5.391 ± 0.086	7.184GluLeu: 7.184 ± 0.102	1.528GluMet: 1.528 ± 0.037	3.986GluAsn: 3.986 ± 0.064	2.24GluPro: 2.24 ± 0.057	3.304GluGln: 3.304 ± 0.065	3.009GluArg: 3.009 ± 0.053	3.889GluSer: 3.889 ± 0.065	4.075GluThr: 4.075 ± 0.067	4.769GluVal: 4.769 ± 0.074	0.879GluTrp: 0.879 ± 0.026	2.141GluTyr: 2.141 ± 0.042	0.045GluXaa: 0.045 ± 0.005
Phe	2.868PheAla: 2.868 ± 0.052	0.597PheCys: 0.597 ± 0.023	2.38PheAsp: 2.38 ± 0.05	2.354PheGlu: 2.354 ± 0.044	1.879PhePhe: 1.879 ± 0.043	2.936PheGly: 2.936 ± 0.055	0.77PheHis: 0.77 ± 0.026	2.722PheIle: 2.722 ± 0.054	1.782PheLys: 1.782 ± 0.042	4.358PheLeu: 4.358 ± 0.07	0.795PheMet: 0.795 ± 0.026	2.047PheAsn: 2.047 ± 0.05	1.958PhePro: 1.958 ± 0.041	1.874PheGln: 1.874 ± 0.038	1.747PheArg: 1.747 ± 0.037	3.29PheSer: 3.29 ± 0.059	2.368PheThr: 2.368 ± 0.048	2.438PheVal: 2.438 ± 0.047	0.741PheTrp: 0.741 ± 0.025	1.512PheTyr: 1.512 ± 0.033	0.034PheXaa: 0.034 ± 0.005
Gly	4.424GlyAla: 4.424 ± 0.08	0.822GlyCys: 0.822 ± 0.028	3.523GlyAsp: 3.523 ± 0.097	4.55GlyGlu: 4.55 ± 0.075	3.01GlyPhe: 3.01 ± 0.051	4.984GlyGly: 4.984 ± 0.117	1.164GlyHis: 1.164 ± 0.036	5.454GlyIle: 5.454 ± 0.08	4.761GlyLys: 4.761 ± 0.07	6.529GlyLeu: 6.529 ± 0.086	1.577GlyMet: 1.577 ± 0.042	3.588GlyAsn: 3.588 ± 0.105	1.379GlyPro: 1.379 ± 0.04	2.499GlyGln: 2.499 ± 0.049	2.912GlyArg: 2.912 ± 0.052	3.967GlySer: 3.967 ± 0.067	3.6GlyThr: 3.6 ± 0.07	4.564GlyVal: 4.564 ± 0.066	1.112GlyTrp: 1.112 ± 0.032	2.315GlyTyr: 2.315 ± 0.042	0.037GlyXaa: 0.037 ± 0.006
His	0.833HisAla: 0.833 ± 0.028	0.239HisCys: 0.239 ± 0.014	0.611HisAsp: 0.611 ± 0.024	0.789HisGlu: 0.789 ± 0.027	0.76HisPhe: 0.76 ± 0.027	1.077HisGly: 1.077 ± 0.036	0.543HisHis: 0.543 ± 0.029	1.155HisIle: 1.155 ± 0.029	0.87HisLys: 0.87 ± 0.028	1.976HisLeu: 1.976 ± 0.041	0.159HisMet: 0.159 ± 0.01	0.775HisAsn: 0.775 ± 0.023	1.262HisPro: 1.262 ± 0.03	1.07HisGln: 1.07 ± 0.032	0.924HisArg: 0.924 ± 0.028	1.107HisSer: 1.107 ± 0.034	0.789HisThr: 0.789 ± 0.026	0.69HisVal: 0.69 ± 0.027	0.292HisTrp: 0.292 ± 0.016	0.642HisTyr: 0.642 ± 0.021	0.007HisXaa: 0.007 ± 0.002
Ile	6.064IleAla: 6.064 ± 0.072	0.831IleCys: 0.831 ± 0.028	4.167IleAsp: 4.167 ± 0.057	4.928IleGlu: 4.928 ± 0.07	3.278IlePhe: 3.278 ± 0.056	4.749IleGly: 4.749 ± 0.072	1.184IleHis: 1.184 ± 0.035	5.204IleIle: 5.204 ± 0.077	3.931IleLys: 3.931 ± 0.062	7.355IleLeu: 7.355 ± 0.087	1.119IleMet: 1.119 ± 0.032	4.263IleAsn: 4.263 ± 0.078	3.745IlePro: 3.745 ± 0.062	2.984IleGln: 2.984 ± 0.053	2.995IleArg: 2.995 ± 0.051	5.575IleSer: 5.575 ± 0.083	4.145IleThr: 4.145 ± 0.077	4.512IleVal: 4.512 ± 0.066	0.969IleTrp: 0.969 ± 0.031	2.413IleTyr: 2.413 ± 0.049	0.046IleXaa: 0.046 ± 0.006
Lys	3.994LysAla: 3.994 ± 0.066	0.459LysCys: 0.459 ± 0.02	2.505LysAsp: 2.505 ± 0.047	3.649LysGlu: 3.649 ± 0.06	2.463LysPhe: 2.463 ± 0.048	2.988LysGly: 2.988 ± 0.05	0.864LysHis: 0.864 ± 0.026	4.859LysIle: 4.859 ± 0.073	3.658LysLys: 3.658 ± 0.072	6.658LysLeu: 6.658 ± 0.097	1.231LysMet: 1.231 ± 0.034	3.161LysAsn: 3.161 ± 0.059	2.656LysPro: 2.656 ± 0.051	2.993LysGln: 2.993 ± 0.05	2.463LysArg: 2.463 ± 0.051	3.891LysSer: 3.891 ± 0.07	3.319LysThr: 3.319 ± 0.061	3.85LysVal: 3.85 ± 0.07	0.706LysTrp: 0.706 ± 0.022	1.973LysTyr: 1.973 ± 0.044	0.031LysXaa: 0.031 ± 0.005
Leu	8.318LeuAla: 8.318 ± 0.098	0.943LeuCys: 0.943 ± 0.03	5.283LeuAsp: 5.283 ± 0.075	8.026LeuGlu: 8.026 ± 0.104	3.834LeuPhe: 3.834 ± 0.066	7.26LeuGly: 7.26 ± 0.103	1.631LeuHis: 1.631 ± 0.043	7.169LeuIle: 7.169 ± 0.105	6.185LeuLys: 6.185 ± 0.082	10.351LeuLeu: 10.351 ± 0.13	2.104LeuMet: 2.104 ± 0.043	4.906LeuAsn: 4.906 ± 0.066	5.375LeuPro: 5.375 ± 0.077	4.723LeuGln: 4.723 ± 0.078	4.687LeuArg: 4.687 ± 0.065	7.382LeuSer: 7.382 ± 0.089	6.178LeuThr: 6.178 ± 0.069	6.557LeuVal: 6.557 ± 0.081	1.348LeuTrp: 1.348 ± 0.039	2.667LeuTyr: 2.667 ± 0.041	0.047LeuXaa: 0.047 ± 0.006
Met	1.684MetAla: 1.684 ± 0.039	0.11MetCys: 0.11 ± 0.008	0.736MetAsp: 0.736 ± 0.026	1.225MetGlu: 1.225 ± 0.03	0.658MetPhe: 0.658 ± 0.025	1.453MetGly: 1.453 ± 0.03	0.23MetHis: 0.23 ± 0.013	1.209MetIle: 1.209 ± 0.032	1.21MetLys: 1.21 ± 0.034	1.914MetLeu: 1.914 ± 0.043	0.481MetMet: 0.481 ± 0.018	0.95MetAsn: 0.95 ± 0.028	0.914MetPro: 0.914 ± 0.025	0.768MetGln: 0.768 ± 0.024	0.908MetArg: 0.908 ± 0.024	1.469MetSer: 1.469 ± 0.036	1.308MetThr: 1.308 ± 0.031	1.31MetVal: 1.31 ± 0.032	0.174MetTrp: 0.174 ± 0.011	0.406MetTyr: 0.406 ± 0.019	0.022MetXaa: 0.022 ± 0.004
Asn	2.838AsnAla: 2.838 ± 0.061	0.586AsnCys: 0.586 ± 0.02	2.13AsnAsp: 2.13 ± 0.057	2.274AsnGlu: 2.274 ± 0.043	2.552AsnPhe: 2.552 ± 0.052	2.995AsnGly: 2.995 ± 0.077	0.995AsnHis: 0.995 ± 0.029	4.144AsnIle: 4.144 ± 0.093	2.356AsnLys: 2.356 ± 0.05	6.569AsnLeu: 6.569 ± 0.101	0.843AsnMet: 0.843 ± 0.024	2.975AsnAsn: 2.975 ± 0.063	2.852AsnPro: 2.852 ± 0.046	3.214AsnGln: 3.214 ± 0.051	2.204AsnArg: 2.204 ± 0.042	3.978AsnSer: 3.978 ± 0.072	2.538AsnThr: 2.538 ± 0.049	2.33AsnVal: 2.33 ± 0.047	0.887AsnTrp: 0.887 ± 0.028	2.156AsnTyr: 2.156 ± 0.05	0.032AsnXaa: 0.032 ± 0.005
Pro	2.418ProAla: 2.418 ± 0.053	0.351ProCys: 0.351 ± 0.017	2.772ProAsp: 2.772 ± 0.055	4.387ProGlu: 4.387 ± 0.079	1.69ProPhe: 1.69 ± 0.04	2.865ProGly: 2.865 ± 0.061	0.777ProHis: 0.777 ± 0.027	3.15ProIle: 3.15 ± 0.056	2.377ProLys: 2.377 ± 0.048	4.198ProLeu: 4.198 ± 0.062	0.723ProMet: 0.723 ± 0.023	2.367ProAsn: 2.367 ± 0.043	2.029ProPro: 2.029 ± 0.054	2.199ProGln: 2.199 ± 0.045	1.581ProArg: 1.581 ± 0.041	2.52ProSer: 2.52 ± 0.045	2.758ProThr: 2.758 ± 0.056	2.89ProVal: 2.89 ± 0.057	0.575ProTrp: 0.575 ± 0.025	1.375ProTyr: 1.375 ± 0.035	0.019ProXaa: 0.019 ± 0.004
Gln	3.486GlnAla: 3.486 ± 0.061	0.314GlnCys: 0.314 ± 0.018	2.058GlnAsp: 2.058 ± 0.036	3.932GlnGlu: 3.932 ± 0.062	1.589GlnPhe: 1.589 ± 0.044	2.954GlnGly: 2.954 ± 0.054	0.719GlnHis: 0.719 ± 0.024	3.578GlnIle: 3.578 ± 0.056	3.442GlnLys: 3.442 ± 0.054	5.157GlnLeu: 5.157 ± 0.077	0.916GlnMet: 0.916 ± 0.028	2.454GlnAsn: 2.454 ± 0.046	2.199GlnPro: 2.199 ± 0.05	3.148GlnGln: 3.148 ± 0.08	2.219GlnArg: 2.219 ± 0.045	2.474GlnSer: 2.474 ± 0.047	2.842GlnThr: 2.842 ± 0.055	3.334GlnVal: 3.334 ± 0.055	0.58GlnTrp: 0.58 ± 0.021	1.212GlnTyr: 1.212 ± 0.03	0.019GlnXaa: 0.019 ± 0.004
Arg	2.589ArgAla: 2.589 ± 0.046	0.462ArgCys: 0.462 ± 0.02	2.239ArgAsp: 2.239 ± 0.043	3.183ArgGlu: 3.183 ± 0.054	1.887ArgPhe: 1.887 ± 0.039	2.78ArgGly: 2.78 ± 0.052	0.791ArgHis: 0.791 ± 0.023	3.078ArgIle: 3.078 ± 0.047	2.55ArgLys: 2.55 ± 0.049	4.99ArgLeu: 4.99 ± 0.071	0.933ArgMet: 0.933 ± 0.03	2.133ArgAsn: 2.133 ± 0.041	1.754ArgPro: 1.754 ± 0.044	2.677ArgGln: 2.677 ± 0.049	2.537ArgArg: 2.537 ± 0.06	2.796ArgSer: 2.796 ± 0.049	2.056ArgThr: 2.056 ± 0.043	2.905ArgVal: 2.905 ± 0.052	0.673ArgTrp: 0.673 ± 0.023	1.71ArgTyr: 1.71 ± 0.035	0.02ArgXaa: 0.02 ± 0.004
Ser	3.938SerAla: 3.938 ± 0.055	0.687SerCys: 0.687 ± 0.024	3.369SerAsp: 3.369 ± 0.051	4.464SerGlu: 4.464 ± 0.068	2.751SerPhe: 2.751 ± 0.054	4.859SerGly: 4.859 ± 0.086	1.237SerHis: 1.237 ± 0.032	4.336SerIle: 4.336 ± 0.061	3.32SerLys: 3.32 ± 0.058	7.262SerLeu: 7.262 ± 0.073	1.265SerMet: 1.265 ± 0.034	3.183SerAsn: 3.183 ± 0.06	3.059SerPro: 3.059 ± 0.053	3.73SerGln: 3.73 ± 0.062	3.036SerArg: 3.036 ± 0.055	4.481SerSer: 4.481 ± 0.075	3.332SerThr: 3.332 ± 0.064	3.78SerVal: 3.78 ± 0.061	0.962SerTrp: 0.962 ± 0.028	2.068SerTyr: 2.068 ± 0.04	0.025SerXaa: 0.025 ± 0.005
Thr	4.06ThrAla: 4.06 ± 0.074	0.449ThrCys: 0.449 ± 0.019	2.716ThrAsp: 2.716 ± 0.05	3.965ThrGlu: 3.965 ± 0.064	2.166ThrPhe: 2.166 ± 0.041	4.28ThrGly: 4.28 ± 0.079	0.878ThrHis: 0.878 ± 0.029	4.286ThrIle: 4.286 ± 0.067	2.922ThrLys: 2.922 ± 0.049	5.661ThrLeu: 5.661 ± 0.066	0.863ThrMet: 0.863 ± 0.027	2.816ThrAsn: 2.816 ± 0.059	3.137ThrPro: 3.137 ± 0.069	2.456ThrGln: 2.456 ± 0.048	2.017ThrArg: 2.017 ± 0.037	3.287ThrSer: 3.287 ± 0.057	3.536ThrThr: 3.536 ± 0.078	3.831ThrVal: 3.831 ± 0.074	0.651ThrTrp: 0.651 ± 0.027	1.616ThrTyr: 1.616 ± 0.036	0.031ThrXaa: 0.031 ± 0.005
Val	5.121ValAla: 5.121 ± 0.078	0.668ValCys: 0.668 ± 0.024	3.415ValAsp: 3.415 ± 0.062	4.764ValGlu: 4.764 ± 0.072	2.454ValPhe: 2.454 ± 0.048	4.672ValGly: 4.672 ± 0.066	0.892ValHis: 0.892 ± 0.029	4.485ValIle: 4.485 ± 0.062	3.874ValLys: 3.874 ± 0.067	5.817ValLeu: 5.817 ± 0.078	1.369ValMet: 1.369 ± 0.031	3.165ValAsn: 3.165 ± 0.048	2.502ValPro: 2.502 ± 0.053	2.3ValGln: 2.3 ± 0.051	2.799ValArg: 2.799 ± 0.049	4.188ValSer: 4.188 ± 0.061	3.52ValThr: 3.52 ± 0.063	4.682ValVal: 4.682 ± 0.069	0.842ValTrp: 0.842 ± 0.033	1.864ValTyr: 1.864 ± 0.034	0.024ValXaa: 0.024 ± 0.004
Trp	0.762TrpAla: 0.762 ± 0.025	0.163TrpCys: 0.163 ± 0.011	0.637TrpAsp: 0.637 ± 0.026	1.037TrpGlu: 1.037 ± 0.03	0.58TrpPhe: 0.58 ± 0.022	0.98TrpGly: 0.98 ± 0.032	0.301TrpHis: 0.301 ± 0.014	0.829TrpIle: 0.829 ± 0.027	0.757TrpLys: 0.757 ± 0.026	1.756TrpLeu: 1.756 ± 0.046	0.321TrpMet: 0.321 ± 0.017	0.648TrpAsn: 0.648 ± 0.021	0.363TrpPro: 0.363 ± 0.018	1.143TrpGln: 1.143 ± 0.033	0.742TrpArg: 0.742 ± 0.026	0.873TrpSer: 0.873 ± 0.025	0.475TrpThr: 0.475 ± 0.019	0.959TrpVal: 0.959 ± 0.029	0.224TrpTrp: 0.224 ± 0.013	0.433TrpTyr: 0.433 ± 0.018	0.007TrpXaa: 0.007 ± 0.002
Tyr	1.786TyrAla: 1.786 ± 0.036	0.414TyrCys: 0.414 ± 0.018	1.56TyrAsp: 1.56 ± 0.036	1.887TyrGlu: 1.887 ± 0.043	1.522TyrPhe: 1.522 ± 0.041	2.157TyrGly: 2.157 ± 0.045	0.691TyrHis: 0.691 ± 0.023	2.013TyrIle: 2.013 ± 0.041	1.515TyrLys: 1.515 ± 0.04	3.711TyrLeu: 3.711 ± 0.054	0.464TyrMet: 0.464 ± 0.021	1.462TyrAsn: 1.462 ± 0.039	1.656TyrPro: 1.656 ± 0.04	2.365TyrGln: 2.365 ± 0.053	1.881TyrArg: 1.881 ± 0.041	2.179TyrSer: 2.179 ± 0.043	1.509TyrThr: 1.509 ± 0.035	1.604TyrVal: 1.604 ± 0.034	0.575TyrTrp: 0.575 ± 0.021	1.291TyrTyr: 1.291 ± 0.037	0.009TyrXaa: 0.009 ± 0.002
Xaa	0.022XaaAla: 0.022 ± 0.004	0.007XaaCys: 0.007 ± 0.002	0.014XaaAsp: 0.014 ± 0.003	0.021XaaGlu: 0.021 ± 0.004	0.034XaaPhe: 0.034 ± 0.006	0.02XaaGly: 0.02 ± 0.004	0.009XaaHis: 0.009 ± 0.003	0.034XaaIle: 0.034 ± 0.005	0.035XaaLys: 0.035 ± 0.005	0.057XaaLeu: 0.057 ± 0.007	0.025XaaMet: 0.025 ± 0.004	0.031XaaAsn: 0.031 ± 0.005	0.025XaaPro: 0.025 ± 0.004	0.021XaaGln: 0.021 ± 0.004	0.023XaaArg: 0.023 ± 0.004	0.046XaaSer: 0.046 ± 0.006	0.024XaaThr: 0.024 ± 0.004	0.033XaaVal: 0.033 ± 0.005	0.011XaaTrp: 0.011 ± 0.003	0.021XaaTyr: 0.021 ± 0.004	0.011XaaXaa: 0.011 ± 0.005

Statistics based on 4827 proteins (1336359 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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