Liberibacter crescens (strain BT-1)
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1376 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0EVL0|L0EVL0_LIBCB UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase OS=Liberibacter crescens (strain BT-1) OX=1215343 GN=murG PE=3 SV=1
MM1 pKa = 8.05 DD2 pKa = 4.35 NCSINSSVNVNNDD15 pKa = 3.13 MEE17 pKa = 4.69 VDD19 pKa = 3.58 PLYY22 pKa = 11.0 CSIRR26 pKa = 11.84 AYY28 pKa = 10.35 EE29 pKa = 4.08 EE30 pKa = 4.23 ACDD33 pKa = 3.76 FLNYY37 pKa = 9.9 TDD39 pKa = 5.02 IEE41 pKa = 4.42 TDD43 pKa = 3.05 NSEE46 pKa = 4.11 FFSNNVISQQISVLEE61 pKa = 4.1 SWTFAVKK68 pKa = 8.95 TAQSALDD75 pKa = 3.78 ALRR78 pKa = 11.84 LAIKK82 pKa = 10.05 EE83 pKa = 4.01 MEE85 pKa = 4.4 YY86 pKa = 11.21 VDD88 pKa = 4.66 VDD90 pKa = 3.72 PLIASMIRR98 pKa = 11.84 AAFGYY103 pKa = 10.26 FSMHH107 pKa = 5.52 IQQ109 pKa = 3.17
Molecular weight: 12.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.961
Patrickios 0.54
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|L0EY55|L0EY55_LIBCB Biotin synthase OS=Liberibacter crescens (strain BT-1) OX=1215343 GN=bioB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.64 RR3 pKa = 11.84 TFNPSNVIRR12 pKa = 11.84 KK13 pKa = 8.64 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.26 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.39 VISARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1376
0
1376
419917
37
2830
305.2
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.818 ± 0.059
1.122 ± 0.02
5.09 ± 0.057
5.995 ± 0.069
4.724 ± 0.057
6.291 ± 0.056
2.156 ± 0.027
8.982 ± 0.069
6.424 ± 0.053
9.929 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.479 ± 0.027
4.874 ± 0.053
3.836 ± 0.041
3.393 ± 0.04
4.968 ± 0.053
7.737 ± 0.065
4.746 ± 0.045
6.358 ± 0.063
0.999 ± 0.022
3.08 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here