Synechococcus sp. MIT S9508

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164C9S5|A0A164C9S5_9SYNE Imidazole glycerol phosphate synthase subunit HisF OS=Synechococcus sp. MIT S9508 OX=1801629 GN=hisF PE=3 SV=1
MM1 pKa = 7.58TFTGQYY7 pKa = 11.09VNIQSHH13 pKa = 5.82SSGSQGSTTALRR25 pKa = 11.84NTSFIATSDD34 pKa = 3.07LGTRR38 pKa = 11.84IDD40 pKa = 4.41INASSNSNGGWWWNSGSSNTTGISNTSIEE69 pKa = 4.47TGSGNDD75 pKa = 3.71VININANANSNGGGWWWRR93 pKa = 11.84RR94 pKa = 11.84GGNADD99 pKa = 4.74SVGIDD104 pKa = 3.36NNSSINTGAGNDD116 pKa = 3.59SVYY119 pKa = 11.32VNAFASGAATNAWAIRR135 pKa = 11.84DD136 pKa = 3.65SSIDD140 pKa = 3.45VGSGDD145 pKa = 5.04DD146 pKa = 3.87VVSLNATTFQTQRR159 pKa = 11.84RR160 pKa = 11.84YY161 pKa = 10.67DD162 pKa = 3.83PAWGSEE168 pKa = 3.81NSSIDD173 pKa = 3.54LGSGNDD179 pKa = 3.2RR180 pKa = 11.84LFINANASGQGEE192 pKa = 4.08MRR194 pKa = 11.84AFGALDD200 pKa = 3.39STIDD204 pKa = 3.56AGSGDD209 pKa = 4.13DD210 pKa = 4.8AISINASANSNNGWWWWGGGNDD232 pKa = 3.67PATAKK237 pKa = 10.67GLANSDD243 pKa = 3.18VDD245 pKa = 4.47LGSGNDD251 pKa = 3.76FLSVSASTNGGISNATAVEE270 pKa = 4.29DD271 pKa = 3.77SSIDD275 pKa = 3.4AGSGNDD281 pKa = 3.59VININTNARR290 pKa = 11.84ANGGGWRR297 pKa = 11.84WWNRR301 pKa = 11.84GGNPATAKK309 pKa = 10.79GLDD312 pKa = 3.4NSEE315 pKa = 4.06ISTGDD320 pKa = 3.41GQDD323 pKa = 3.57SISINTTATSNGGGWWWGGGGNADD347 pKa = 4.71SVGIDD352 pKa = 3.36NNSSINTGADD362 pKa = 3.14NDD364 pKa = 4.29SVYY367 pKa = 11.37VNAFASGAATNAWAIRR383 pKa = 11.84DD384 pKa = 3.65SSIDD388 pKa = 3.45VGSGDD393 pKa = 5.04DD394 pKa = 3.87VVSLNATTFQTQRR407 pKa = 11.84RR408 pKa = 11.84YY409 pKa = 10.62DD410 pKa = 3.48PAYY413 pKa = 9.77GSEE416 pKa = 4.08NSSIDD421 pKa = 3.54LGSGNDD427 pKa = 3.2RR428 pKa = 11.84LFINANASGQGDD440 pKa = 3.1IRR442 pKa = 11.84AFGALDD448 pKa = 3.43TTIDD452 pKa = 3.75AGSGDD457 pKa = 4.13DD458 pKa = 4.8AISINASANNNNWGWGNAATATGIGSTEE486 pKa = 3.72ISTGSGDD493 pKa = 4.31DD494 pKa = 3.59SLSINATAHH503 pKa = 5.31GRR505 pKa = 11.84QNEE508 pKa = 4.12WSSQSSWGYY517 pKa = 10.38DD518 pKa = 3.32YY519 pKa = 10.99SYY521 pKa = 11.5SGATQWSSEE530 pKa = 4.03YY531 pKa = 10.71SHH533 pKa = 6.67SANLAFGGGWWGSSSLSYY551 pKa = 10.64GYY553 pKa = 9.57QSKK556 pKa = 10.9YY557 pKa = 11.05DD558 pKa = 3.67YY559 pKa = 10.71DD560 pKa = 3.76YY561 pKa = 11.0EE562 pKa = 4.31YY563 pKa = 11.4ANQGHH568 pKa = 6.26YY569 pKa = 10.04KK570 pKa = 10.15SSHH573 pKa = 4.92SSRR576 pKa = 11.84NVDD579 pKa = 2.72ISGGEE584 pKa = 3.92SYY586 pKa = 11.21GAINSSIDD594 pKa = 3.71LGSGDD599 pKa = 3.27NTATVNVLAGNQAVGFRR616 pKa = 11.84ATSLNSGNGDD626 pKa = 3.43DD627 pKa = 5.03SFSLSVNANGEE638 pKa = 3.84RR639 pKa = 11.84SYY641 pKa = 11.33SYY643 pKa = 10.86TSDD646 pKa = 3.5YY647 pKa = 11.17EE648 pKa = 4.43YY649 pKa = 11.23ASSGYY654 pKa = 10.55DD655 pKa = 2.66IGSNSGEE662 pKa = 4.02SSSWRR667 pKa = 11.84SYY669 pKa = 9.86QNNSRR674 pKa = 11.84AWWWGNSQSQSEE686 pKa = 4.72HH687 pKa = 5.67ASKK690 pKa = 10.34WDD692 pKa = 3.39NSWNHH697 pKa = 4.24SHH699 pKa = 6.94EE700 pKa = 4.25YY701 pKa = 9.64SNKK704 pKa = 9.64SASEE708 pKa = 3.84HH709 pKa = 7.13SNTQRR714 pKa = 11.84FGSSLGSEE722 pKa = 4.22NSTIDD727 pKa = 5.03LGNGDD732 pKa = 4.19NNAEE736 pKa = 3.92ISVKK740 pKa = 10.39GGEE743 pKa = 4.16RR744 pKa = 11.84ALGLISSDD752 pKa = 3.65LLTGSGSDD760 pKa = 3.76TISIDD765 pKa = 3.2TSAEE769 pKa = 3.8GLVEE773 pKa = 3.88YY774 pKa = 10.99SNTSKK779 pKa = 11.05GSNSNSYY786 pKa = 10.98SNIYY790 pKa = 10.16NYY792 pKa = 10.95SNSNSHH798 pKa = 6.44EE799 pKa = 3.93YY800 pKa = 10.39SYY802 pKa = 11.86SSARR806 pKa = 11.84WYY808 pKa = 10.63GHH810 pKa = 5.38NQSSSEE816 pKa = 3.75GNYY819 pKa = 10.04DD820 pKa = 3.5YY821 pKa = 11.27KK822 pKa = 11.38SQGEE826 pKa = 4.3YY827 pKa = 10.32TNQGQSNWDD836 pKa = 3.28NEE838 pKa = 3.99YY839 pKa = 10.76SRR841 pKa = 11.84TYY843 pKa = 10.73RR844 pKa = 11.84FGVSIGAEE852 pKa = 4.06DD853 pKa = 3.77SSIDD857 pKa = 4.17AGDD860 pKa = 3.84GNNSINLQSIGGEE873 pKa = 3.94LAIALAGSSITAGSGDD889 pKa = 4.31DD890 pKa = 3.41SLAIIGLAEE899 pKa = 4.75GEE901 pKa = 3.84DD902 pKa = 3.95SYY904 pKa = 12.06INRR907 pKa = 11.84KK908 pKa = 9.24KK909 pKa = 11.07SNSTWNHH916 pKa = 6.4DD917 pKa = 3.03SWNSNKK923 pKa = 9.72RR924 pKa = 11.84SEE926 pKa = 4.48EE927 pKa = 3.7YY928 pKa = 10.4SRR930 pKa = 11.84SYY932 pKa = 9.9TSAYY936 pKa = 8.42YY937 pKa = 8.88WYY939 pKa = 10.69NRR941 pKa = 11.84IEE943 pKa = 4.45EE944 pKa = 4.26YY945 pKa = 10.8SSNHH949 pKa = 5.32EE950 pKa = 4.23SEE952 pKa = 4.58SDD954 pKa = 3.5GFSISLSRR962 pKa = 11.84YY963 pKa = 6.15NQNYY967 pKa = 7.72EE968 pKa = 3.49YY969 pKa = 11.3SRR971 pKa = 11.84IKK973 pKa = 10.74RR974 pKa = 11.84FGTAYY979 pKa = 10.04GAQKK983 pKa = 10.87SSINLGNGDD992 pKa = 3.81NEE994 pKa = 4.29ASISANGGEE1003 pKa = 4.39SAEE1006 pKa = 4.18ALSDD1010 pKa = 3.73SSLEE1014 pKa = 4.09TGKK1017 pKa = 11.11GDD1019 pKa = 4.69DD1020 pKa = 4.52VISLSATALGEE1031 pKa = 3.8NSLINKK1037 pKa = 9.33NEE1039 pKa = 3.76YY1040 pKa = 10.33LSEE1043 pKa = 4.22HH1044 pKa = 6.4SSVYY1048 pKa = 8.35SHH1050 pKa = 6.95KK1051 pKa = 10.71SEE1053 pKa = 3.88YY1054 pKa = 10.32SYY1056 pKa = 11.04QGEE1059 pKa = 4.27GLNQSRR1065 pKa = 11.84YY1066 pKa = 8.86GWGWWNPTSEE1076 pKa = 4.76SSHH1079 pKa = 6.46SYY1081 pKa = 9.56NNKK1084 pKa = 9.61GNHH1087 pKa = 5.78NSQGNNQSLFIEE1099 pKa = 5.07DD1100 pKa = 4.35YY1101 pKa = 10.75EE1102 pKa = 4.32NSLIQKK1108 pKa = 9.51LGSAIGANRR1117 pKa = 11.84STIDD1121 pKa = 3.25TGSGDD1126 pKa = 4.33DD1127 pKa = 5.27LLTIDD1132 pKa = 3.81AKK1134 pKa = 11.21GYY1136 pKa = 8.57TEE1138 pKa = 4.92AVGIKK1143 pKa = 10.14NSSIDD1148 pKa = 3.58LGKK1151 pKa = 10.84GDD1153 pKa = 4.85DD1154 pKa = 3.73VFTISSSAFGFTSYY1168 pKa = 10.62LRR1170 pKa = 11.84QGLGSYY1176 pKa = 10.03DD1177 pKa = 3.38YY1178 pKa = 11.12SYY1180 pKa = 11.14DD1181 pKa = 3.8YY1182 pKa = 10.93QSSGSQSGEE1191 pKa = 3.49YY1192 pKa = 9.9SNSYY1196 pKa = 9.4RR1197 pKa = 11.84YY1198 pKa = 9.98SYY1200 pKa = 9.97PWWSYY1205 pKa = 8.11GRR1207 pKa = 11.84SYY1209 pKa = 10.79SGSYY1213 pKa = 9.54GSQWNYY1219 pKa = 9.9GYY1221 pKa = 11.03GYY1223 pKa = 10.12ASSGTWSYY1231 pKa = 12.11NEE1233 pKa = 4.57DD1234 pKa = 2.81SWLINTGIAKK1244 pKa = 10.15GAVNSTIDD1252 pKa = 3.35TGKK1255 pKa = 10.83GDD1257 pKa = 4.41DD1258 pKa = 4.79NISITARR1265 pKa = 11.84ASSKK1269 pKa = 9.6ATAIEE1274 pKa = 4.27DD1275 pKa = 4.17SEE1277 pKa = 4.72VLTGDD1282 pKa = 3.47GDD1284 pKa = 4.24DD1285 pKa = 3.86SVEE1288 pKa = 3.95VSAAAEE1294 pKa = 3.89EE1295 pKa = 4.32DD1296 pKa = 3.11ATAIEE1301 pKa = 4.6DD1302 pKa = 4.25SEE1304 pKa = 4.87VSTGDD1309 pKa = 3.15GDD1311 pKa = 4.8DD1312 pKa = 3.78SVEE1315 pKa = 3.95VSAAAEE1321 pKa = 3.89EE1322 pKa = 4.32DD1323 pKa = 3.11ATAIEE1328 pKa = 4.6DD1329 pKa = 4.25SEE1331 pKa = 4.86VSTGDD1336 pKa = 3.5GEE1338 pKa = 4.84DD1339 pKa = 3.85LIEE1342 pKa = 4.33VEE1344 pKa = 4.69STAEE1348 pKa = 4.05SSDD1351 pKa = 3.5EE1352 pKa = 4.11ASDD1355 pKa = 3.47EE1356 pKa = 4.33GVAKK1360 pKa = 10.7GLVNTTIDD1368 pKa = 4.04AGADD1372 pKa = 3.35DD1373 pKa = 5.45DD1374 pKa = 4.44ALLMEE1379 pKa = 4.74VTGEE1383 pKa = 3.92TLAIGMEE1390 pKa = 3.93NSTLNVGDD1398 pKa = 4.12SDD1400 pKa = 5.83DD1401 pKa = 4.6IIDD1404 pKa = 4.63VIVEE1408 pKa = 4.05ATNEE1412 pKa = 3.9EE1413 pKa = 4.23DD1414 pKa = 4.07GEE1416 pKa = 4.62AIGLSGSTISSGGGDD1431 pKa = 3.27DD1432 pKa = 4.13SIYY1435 pKa = 10.44VEE1437 pKa = 4.22VWGEE1441 pKa = 3.97SSAKK1445 pKa = 10.8AMVDD1449 pKa = 3.46SLIEE1453 pKa = 4.44AGDD1456 pKa = 3.73GDD1458 pKa = 5.27DD1459 pKa = 5.05YY1460 pKa = 11.3ITIDD1464 pKa = 4.22GEE1466 pKa = 4.55IEE1468 pKa = 4.08NSTIDD1473 pKa = 4.27AGDD1476 pKa = 3.76GDD1478 pKa = 5.1DD1479 pKa = 5.31VVDD1482 pKa = 5.71LYY1484 pKa = 11.16GTGDD1488 pKa = 3.27ATVQGGAGEE1497 pKa = 4.72DD1498 pKa = 3.82EE1499 pKa = 4.71LNGDD1503 pKa = 4.23EE1504 pKa = 6.03GNDD1507 pKa = 3.76TLLGGADD1514 pKa = 3.5NDD1516 pKa = 3.83ILNGYY1521 pKa = 8.79SGNDD1525 pKa = 3.38TLKK1528 pKa = 10.91GGEE1531 pKa = 4.43GDD1533 pKa = 5.07DD1534 pKa = 4.57ILDD1537 pKa = 4.3AGIGLDD1543 pKa = 3.81KK1544 pKa = 11.0LYY1546 pKa = 11.16GGDD1549 pKa = 4.31GEE1551 pKa = 5.03DD1552 pKa = 3.72TFILNLGEE1560 pKa = 4.67GYY1562 pKa = 8.87ATIYY1566 pKa = 10.91DD1567 pKa = 3.81FVIGEE1572 pKa = 4.93DD1573 pKa = 3.03ILEE1576 pKa = 4.32FSDD1579 pKa = 3.49MGDD1582 pKa = 2.39ITMEE1586 pKa = 4.12YY1587 pKa = 10.09KK1588 pKa = 10.69DD1589 pKa = 4.52KK1590 pKa = 11.61NSLFYY1595 pKa = 11.12SGTDD1599 pKa = 3.38YY1600 pKa = 11.42LGVANGVQLTRR1611 pKa = 11.84QEE1613 pKa = 4.69DD1614 pKa = 3.71EE1615 pKa = 4.32TFAA1618 pKa = 5.62

Molecular weight: 170.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A164CU86|A0A164CU86_9SYNE Uncharacterized protein OS=Synechococcus sp. MIT S9508 OX=1801629 GN=MITS9508_00087 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 8.87RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 5.95TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.64RR38 pKa = 11.84GRR40 pKa = 11.84SRR42 pKa = 11.84LSVV45 pKa = 3.08

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2817

0

2817

737073

29

2175

261.7

28.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.825 ± 0.062

1.293 ± 0.017

5.447 ± 0.041

5.857 ± 0.048

3.183 ± 0.039

8.002 ± 0.046

2.077 ± 0.025

4.577 ± 0.039

3.015 ± 0.042

12.312 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.021

2.859 ± 0.036

5.228 ± 0.036

4.786 ± 0.036

7.062 ± 0.051

6.904 ± 0.046

4.762 ± 0.034

6.99 ± 0.046

1.752 ± 0.033

1.918 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski