Amino acid dipepetide frequency for Synechococcus sp. MIT S9508

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.349AlaAla: 11.349 ± 0.187
1.439AlaCys: 1.439 ± 0.045
5.694AlaAsp: 5.694 ± 0.099
6.252AlaGlu: 6.252 ± 0.114
3.078AlaPhe: 3.078 ± 0.066
7.866AlaGly: 7.866 ± 0.127
1.625AlaHis: 1.625 ± 0.052
4.957AlaIle: 4.957 ± 0.092
2.869AlaLys: 2.869 ± 0.084
12.51AlaLeu: 12.51 ± 0.164
2.737AlaMet: 2.737 ± 0.064
2.313AlaAsn: 2.313 ± 0.058
4.206AlaPro: 4.206 ± 0.102
3.69AlaGln: 3.69 ± 0.08
5.86AlaArg: 5.86 ± 0.087
6.632AlaSer: 6.632 ± 0.087
4.339AlaThr: 4.339 ± 0.08
7.448AlaVal: 7.448 ± 0.103
1.563AlaTrp: 1.563 ± 0.049
1.823AlaTyr: 1.823 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.969CysAla: 0.969 ± 0.034
0.286CysCys: 0.286 ± 0.02
0.788CysAsp: 0.788 ± 0.036
0.689CysGlu: 0.689 ± 0.032
0.482CysPhe: 0.482 ± 0.023
1.212CysGly: 1.212 ± 0.048
0.323CysHis: 0.323 ± 0.02
0.525CysIle: 0.525 ± 0.032
0.365CysLys: 0.365 ± 0.025
1.517CysLeu: 1.517 ± 0.051
0.259CysMet: 0.259 ± 0.023
0.335CysAsn: 0.335 ± 0.023
0.72CysPro: 0.72 ± 0.033
0.44CysGln: 0.44 ± 0.027
1.159CysArg: 1.159 ± 0.041
1.058CysSer: 1.058 ± 0.04
0.53CysThr: 0.53 ± 0.027
0.723CysVal: 0.723 ± 0.038
0.309CysTrp: 0.309 ± 0.02
0.236CysTyr: 0.236 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
5.272AspAla: 5.272 ± 0.092
0.727AspCys: 0.727 ± 0.037
2.88AspAsp: 2.88 ± 0.069
2.943AspGlu: 2.943 ± 0.069
1.625AspPhe: 1.625 ± 0.055
4.681AspGly: 4.681 ± 0.089
1.296AspHis: 1.296 ± 0.044
1.837AspIle: 1.837 ± 0.052
1.27AspLys: 1.27 ± 0.044
7.481AspLeu: 7.481 ± 0.096
0.894AspMet: 0.894 ± 0.034
1.283AspAsn: 1.283 ± 0.039
3.743AspPro: 3.743 ± 0.071
3.373AspGln: 3.373 ± 0.073
4.086AspArg: 4.086 ± 0.098
3.236AspSer: 3.236 ± 0.077
1.986AspThr: 1.986 ± 0.055
3.504AspVal: 3.504 ± 0.067
1.126AspTrp: 1.126 ± 0.04
1.229AspTyr: 1.229 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
6.849GluAla: 6.849 ± 0.106
0.529GluCys: 0.529 ± 0.029
2.618GluAsp: 2.618 ± 0.058
3.431GluGlu: 3.431 ± 0.081
1.381GluPhe: 1.381 ± 0.042
4.308GluGly: 4.308 ± 0.073
1.262GluHis: 1.262 ± 0.047
2.684GluIle: 2.684 ± 0.063
1.889GluLys: 1.889 ± 0.064
7.816GluLeu: 7.816 ± 0.116
1.171GluMet: 1.171 ± 0.042
1.387GluAsn: 1.387 ± 0.047
2.791GluPro: 2.791 ± 0.07
3.789GluGln: 3.789 ± 0.087
5.029GluArg: 5.029 ± 0.103
3.47GluSer: 3.47 ± 0.06
2.822GluThr: 2.822 ± 0.071
3.826GluVal: 3.826 ± 0.079
0.78GluTrp: 0.78 ± 0.032
0.742GluTyr: 0.742 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
2.895PheAla: 2.895 ± 0.058
0.528PheCys: 0.528 ± 0.028
2.005PheAsp: 2.005 ± 0.054
1.856PheGlu: 1.856 ± 0.051
1.138PhePhe: 1.138 ± 0.044
2.831PheGly: 2.831 ± 0.058
0.654PheHis: 0.654 ± 0.031
1.416PheIle: 1.416 ± 0.047
0.981PheLys: 0.981 ± 0.046
3.314PheLeu: 3.314 ± 0.076
0.646PheMet: 0.646 ± 0.029
1.197PheAsn: 1.197 ± 0.042
1.384PhePro: 1.384 ± 0.045
1.319PheGln: 1.319 ± 0.046
1.975PheArg: 1.975 ± 0.056
2.606PheSer: 2.606 ± 0.061
1.661PheThr: 1.661 ± 0.053
2.032PheVal: 2.032 ± 0.061
0.601PheTrp: 0.601 ± 0.032
0.786PheTyr: 0.786 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
6.728GlyAla: 6.728 ± 0.118
1.296GlyCys: 1.296 ± 0.046
4.289GlyAsp: 4.289 ± 0.086
4.381GlyGlu: 4.381 ± 0.08
3.171GlyPhe: 3.171 ± 0.071
6.505GlyGly: 6.505 ± 0.138
1.802GlyHis: 1.802 ± 0.054
3.991GlyIle: 3.991 ± 0.087
2.471GlyLys: 2.471 ± 0.066
10.227GlyLeu: 10.227 ± 0.142
1.931GlyMet: 1.931 ± 0.063
2.091GlyAsn: 2.091 ± 0.061
3.112GlyPro: 3.112 ± 0.068
3.388GlyGln: 3.388 ± 0.076
5.634GlyArg: 5.634 ± 0.098
5.667GlySer: 5.667 ± 0.105
3.844GlyThr: 3.844 ± 0.083
5.822GlyVal: 5.822 ± 0.101
1.772GlyTrp: 1.772 ± 0.057
1.899GlyTyr: 1.899 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.765HisAla: 1.765 ± 0.054
0.425HisCys: 0.425 ± 0.026
1.039HisAsp: 1.039 ± 0.039
0.954HisGlu: 0.954 ± 0.035
0.715HisPhe: 0.715 ± 0.033
1.86HisGly: 1.86 ± 0.06
0.771HisHis: 0.771 ± 0.04
0.672HisIle: 0.672 ± 0.031
0.425HisLys: 0.425 ± 0.029
2.541HisLeu: 2.541 ± 0.078
0.282HisMet: 0.282 ± 0.021
0.559HisAsn: 0.559 ± 0.029
1.571HisPro: 1.571 ± 0.049
1.175HisGln: 1.175 ± 0.045
1.777HisArg: 1.777 ± 0.055
1.275HisSer: 1.275 ± 0.045
0.75HisThr: 0.75 ± 0.033
1.123HisVal: 1.123 ± 0.043
0.567HisTrp: 0.567 ± 0.029
0.524HisTyr: 0.524 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
4.91IleAla: 4.91 ± 0.087
0.63IleCys: 0.63 ± 0.035
3.059IleAsp: 3.059 ± 0.069
3.129IleGlu: 3.129 ± 0.073
1.266IlePhe: 1.266 ± 0.05
4.332IleGly: 4.332 ± 0.101
1.022IleHis: 1.022 ± 0.04
1.401IleIle: 1.401 ± 0.05
1.556IleLys: 1.556 ± 0.049
3.734IleLeu: 3.734 ± 0.083
0.455IleMet: 0.455 ± 0.024
1.761IleAsn: 1.761 ± 0.065
2.618IlePro: 2.618 ± 0.058
2.011IleGln: 2.011 ± 0.051
3.039IleArg: 3.039 ± 0.066
3.508IleSer: 3.508 ± 0.074
2.856IleThr: 2.856 ± 0.064
2.737IleVal: 2.737 ± 0.067
0.738IleTrp: 0.738 ± 0.037
1.011IleTyr: 1.011 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
3.544LysAla: 3.544 ± 0.082
0.228LysCys: 0.228 ± 0.017
1.677LysAsp: 1.677 ± 0.051
1.804LysGlu: 1.804 ± 0.056
0.685LysPhe: 0.685 ± 0.03
2.391LysGly: 2.391 ± 0.062
0.541LysHis: 0.541 ± 0.031
1.278LysIle: 1.278 ± 0.045
1.214LysLys: 1.214 ± 0.056
3.153LysLeu: 3.153 ± 0.086
0.506LysMet: 0.506 ± 0.027
0.851LysAsn: 0.851 ± 0.035
1.884LysPro: 1.884 ± 0.063
1.606LysGln: 1.606 ± 0.05
2.315LysArg: 2.315 ± 0.056
1.937LysSer: 1.937 ± 0.061
1.658LysThr: 1.658 ± 0.05
2.088LysVal: 2.088 ± 0.058
0.26LysTrp: 0.26 ± 0.021
0.528LysTyr: 0.528 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
12.164LeuAla: 12.164 ± 0.146
1.389LeuCys: 1.389 ± 0.049
6.557LeuAsp: 6.557 ± 0.11
8.142LeuGlu: 8.142 ± 0.145
3.418LeuPhe: 3.418 ± 0.083
8.954LeuGly: 8.954 ± 0.123
2.401LeuHis: 2.401 ± 0.076
6.284LeuIle: 6.284 ± 0.107
4.093LeuLys: 4.093 ± 0.094
15.138LeuLeu: 15.138 ± 0.222
2.872LeuMet: 2.872 ± 0.065
3.905LeuAsn: 3.905 ± 0.073
6.788LeuPro: 6.788 ± 0.116
6.176LeuGln: 6.176 ± 0.1
8.366LeuArg: 8.366 ± 0.139
8.492LeuSer: 8.492 ± 0.12
5.629LeuThr: 5.629 ± 0.084
8.779LeuVal: 8.779 ± 0.149
1.749LeuTrp: 1.749 ± 0.057
1.826LeuTyr: 1.826 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.867MetAla: 2.867 ± 0.067
0.123MetCys: 0.123 ± 0.014
1.094MetAsp: 1.094 ± 0.036
1.21MetGlu: 1.21 ± 0.042
0.491MetPhe: 0.491 ± 0.03
1.768MetGly: 1.768 ± 0.056
0.404MetHis: 0.404 ± 0.025
0.882MetIle: 0.882 ± 0.035
0.678MetLys: 0.678 ± 0.03
2.347MetLeu: 2.347 ± 0.056
0.338MetMet: 0.338 ± 0.021
0.685MetAsn: 0.685 ± 0.029
1.374MetPro: 1.374 ± 0.046
0.97MetGln: 0.97 ± 0.033
1.41MetArg: 1.41 ± 0.044
1.463MetSer: 1.463 ± 0.048
1.395MetThr: 1.395 ± 0.047
1.64MetVal: 1.64 ± 0.05
0.129MetTrp: 0.129 ± 0.014
0.236MetTyr: 0.236 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.637AsnAla: 2.637 ± 0.074
0.362AsnCys: 0.362 ± 0.023
1.594AsnAsp: 1.594 ± 0.049
1.298AsnGlu: 1.298 ± 0.042
0.942AsnPhe: 0.942 ± 0.038
2.437AsnGly: 2.437 ± 0.06
0.663AsnHis: 0.663 ± 0.032
1.115AsnIle: 1.115 ± 0.048
0.92AsnLys: 0.92 ± 0.041
3.171AsnLeu: 3.171 ± 0.078
0.491AsnMet: 0.491 ± 0.027
0.984AsnAsn: 0.984 ± 0.047
1.992AsnPro: 1.992 ± 0.049
1.471AsnGln: 1.471 ± 0.05
2.214AsnArg: 2.214 ± 0.048
2.017AsnSer: 2.017 ± 0.067
1.416AsnThr: 1.416 ± 0.044
1.662AsnVal: 1.662 ± 0.044
0.579AsnTrp: 0.579 ± 0.035
0.62AsnTyr: 0.62 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
5.141ProAla: 5.141 ± 0.092
0.585ProCys: 0.585 ± 0.031
3.314ProAsp: 3.314 ± 0.071
3.814ProGlu: 3.814 ± 0.073
1.825ProPhe: 1.825 ± 0.052
4.313ProGly: 4.313 ± 0.093
0.928ProHis: 0.928 ± 0.035
2.243ProIle: 2.243 ± 0.053
1.456ProLys: 1.456 ± 0.052
6.507ProLeu: 6.507 ± 0.111
1.195ProMet: 1.195 ± 0.044
1.319ProAsn: 1.319 ± 0.04
2.325ProPro: 2.325 ± 0.071
2.167ProGln: 2.167 ± 0.062
2.845ProArg: 2.845 ± 0.072
3.738ProSer: 3.738 ± 0.073
2.433ProThr: 2.433 ± 0.053
4.101ProVal: 4.101 ± 0.071
0.959ProTrp: 0.959 ± 0.037
1.075ProTyr: 1.075 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
4.907GlnAla: 4.907 ± 0.088
0.475GlnCys: 0.475 ± 0.027
1.975GlnAsp: 1.975 ± 0.055
2.628GlnGlu: 2.628 ± 0.069
1.174GlnPhe: 1.174 ± 0.042
3.495GlnGly: 3.495 ± 0.067
1.023GlnHis: 1.023 ± 0.042
2.047GlnIle: 2.047 ± 0.05
1.384GlnLys: 1.384 ± 0.046
6.71GlnLeu: 6.71 ± 0.112
0.986GlnMet: 0.986 ± 0.035
1.054GlnAsn: 1.054 ± 0.04
2.713GlnPro: 2.713 ± 0.064
3.325GlnGln: 3.325 ± 0.081
4.366GlnArg: 4.366 ± 0.084
2.944GlnSer: 2.944 ± 0.069
1.969GlnThr: 1.969 ± 0.052
3.222GlnVal: 3.222 ± 0.067
0.866GlnTrp: 0.866 ± 0.041
0.592GlnTyr: 0.592 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
5.035ArgAla: 5.035 ± 0.08
1.096ArgCys: 1.096 ± 0.043
3.605ArgAsp: 3.605 ± 0.071
3.854ArgGlu: 3.854 ± 0.083
2.888ArgPhe: 2.888 ± 0.066
4.533ArgGly: 4.533 ± 0.084
1.719ArgHis: 1.719 ± 0.052
3.385ArgIle: 3.385 ± 0.077
1.946ArgLys: 1.946 ± 0.057
9.45ArgLeu: 9.45 ± 0.141
1.56ArgMet: 1.56 ± 0.046
2.001ArgAsn: 2.001 ± 0.057
3.464ArgPro: 3.464 ± 0.085
3.968ArgGln: 3.968 ± 0.09
6.123ArgArg: 6.123 ± 0.12
5.172ArgSer: 5.172 ± 0.086
3.012ArgThr: 3.012 ± 0.063
4.407ArgVal: 4.407 ± 0.095
1.768ArgTrp: 1.768 ± 0.05
1.631ArgTyr: 1.631 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
6.272SerAla: 6.272 ± 0.089
0.947SerCys: 0.947 ± 0.039
3.993SerAsp: 3.993 ± 0.094
3.872SerGlu: 3.872 ± 0.077
2.367SerPhe: 2.367 ± 0.063
6.257SerGly: 6.257 ± 0.104
1.359SerHis: 1.359 ± 0.047
3.217SerIle: 3.217 ± 0.073
2.057SerLys: 2.057 ± 0.06
8.006SerLeu: 8.006 ± 0.124
1.68SerMet: 1.68 ± 0.048
2.114SerAsn: 2.114 ± 0.061
3.362SerPro: 3.362 ± 0.067
2.69SerGln: 2.69 ± 0.069
4.473SerArg: 4.473 ± 0.084
5.345SerSer: 5.345 ± 0.118
3.643SerThr: 3.643 ± 0.067
4.606SerVal: 4.606 ± 0.084
1.315SerTrp: 1.315 ± 0.044
1.46SerTyr: 1.46 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
5.143ThrAla: 5.143 ± 0.1
0.514ThrCys: 0.514 ± 0.025
2.431ThrAsp: 2.431 ± 0.064
2.35ThrGlu: 2.35 ± 0.049
1.515ThrPhe: 1.515 ± 0.049
4.183ThrGly: 4.183 ± 0.08
0.948ThrHis: 0.948 ± 0.034
2.273ThrIle: 2.273 ± 0.065
1.274ThrLys: 1.274 ± 0.041
5.857ThrLeu: 5.857 ± 0.086
0.906ThrMet: 0.906 ± 0.039
1.357ThrAsn: 1.357 ± 0.046
3.088ThrPro: 3.088 ± 0.069
1.597ThrGln: 1.597 ± 0.041
2.768ThrArg: 2.768 ± 0.065
3.267ThrSer: 3.267 ± 0.08
2.741ThrThr: 2.741 ± 0.064
3.639ThrVal: 3.639 ± 0.082
0.689ThrTrp: 0.689 ± 0.036
1.083ThrTyr: 1.083 ± 0.039
0.001ThrXaa: 0.001 ± 0.001
Val
6.881ValAla: 6.881 ± 0.117
0.775ValCys: 0.775 ± 0.036
4.054ValAsp: 4.054 ± 0.078
4.195ValGlu: 4.195 ± 0.081
2.348ValPhe: 2.348 ± 0.06
5.01ValGly: 5.01 ± 0.089
1.324ValHis: 1.324 ± 0.05
3.914ValIle: 3.914 ± 0.081
2.159ValLys: 2.159 ± 0.057
8.832ValLeu: 8.832 ± 0.133
1.773ValMet: 1.773 ± 0.051
2.249ValAsn: 2.249 ± 0.063
3.203ValPro: 3.203 ± 0.073
2.845ValGln: 2.845 ± 0.068
4.12ValArg: 4.12 ± 0.083
4.541ValSer: 4.541 ± 0.082
3.385ValThr: 3.385 ± 0.08
6.2ValVal: 6.2 ± 0.127
0.937ValTrp: 0.937 ± 0.043
1.156ValTyr: 1.156 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.256TrpAla: 1.256 ± 0.049
0.248TrpCys: 0.248 ± 0.019
0.738TrpAsp: 0.738 ± 0.03
0.745TrpGlu: 0.745 ± 0.034
0.651TrpPhe: 0.651 ± 0.032
1.137TrpGly: 1.137 ± 0.049
0.398TrpHis: 0.398 ± 0.024
0.971TrpIle: 0.971 ± 0.039
0.533TrpLys: 0.533 ± 0.031
2.642TrpLeu: 2.642 ± 0.076
0.505TrpMet: 0.505 ± 0.024
0.578TrpAsn: 0.578 ± 0.029
0.931TrpPro: 0.931 ± 0.036
0.98TrpGln: 0.98 ± 0.045
1.404TrpArg: 1.404 ± 0.048
1.258TrpSer: 1.258 ± 0.05
0.76TrpThr: 0.76 ± 0.036
1.08TrpVal: 1.08 ± 0.043
0.446TrpTrp: 0.446 ± 0.036
0.263TrpTyr: 0.263 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.667TyrAla: 1.667 ± 0.046
0.323TyrCys: 0.323 ± 0.019
1.068TyrAsp: 1.068 ± 0.042
1.027TyrGlu: 1.027 ± 0.037
0.665TyrPhe: 0.665 ± 0.03
1.947TyrGly: 1.947 ± 0.06
0.307TyrHis: 0.307 ± 0.022
0.678TyrIle: 0.678 ± 0.032
0.611TyrLys: 0.611 ± 0.03
2.168TyrLeu: 2.168 ± 0.055
0.316TyrMet: 0.316 ± 0.022
0.564TyrAsn: 0.564 ± 0.032
1.011TyrPro: 1.011 ± 0.037
0.878TyrGln: 0.878 ± 0.036
1.652TyrArg: 1.652 ± 0.05
1.408TyrSer: 1.408 ± 0.06
0.796TyrThr: 0.796 ± 0.039
1.263TyrVal: 1.263 ± 0.041
0.369TyrTrp: 0.369 ± 0.021
0.463TyrTyr: 0.463 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.005XaaXaa: 0.005 ± 0.005
Statistics based on 2817 proteins (737073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski