Achromobacter phage Motura
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 344 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514CT02|A0A514CT02_9CAUD Uncharacterized protein OS=Achromobacter phage Motura OX=2591403 PE=4 SV=1
MM1 pKa = 7.67 ACYY4 pKa = 10.16 DD5 pKa = 3.65 QIIEE9 pKa = 4.59 NFLEE13 pKa = 4.53 HH14 pKa = 6.58 NPEE17 pKa = 4.24 PSDD20 pKa = 3.77 EE21 pKa = 4.13 QVHH24 pKa = 6.06 AFAHH28 pKa = 5.89 ALGVDD33 pKa = 3.61 KK34 pKa = 11.31 EE35 pKa = 4.22 EE36 pKa = 5.0 LEE38 pKa = 4.14 SDD40 pKa = 4.8 FYY42 pKa = 11.92 RR43 pKa = 11.84 MLSEE47 pKa = 3.84 RR48 pKa = 11.84 LGYY51 pKa = 9.99 DD52 pKa = 3.34 VQASPPSLYY61 pKa = 10.43 QGTTYY66 pKa = 10.54 QPQNYY71 pKa = 7.41 KK72 pKa = 9.53 TEE74 pKa = 4.21 PFRR77 pKa = 11.84 VNSSARR83 pKa = 11.84 LHH85 pKa = 6.85 ALTLDD90 pKa = 3.32 QRR92 pKa = 11.84 INDD95 pKa = 3.63 GDD97 pKa = 4.19 YY98 pKa = 11.37 DD99 pKa = 4.31 PEE101 pKa = 3.99 TTPAVKK107 pKa = 9.1 NTINDD112 pKa = 4.13 GEE114 pKa = 4.37 TSPSKK119 pKa = 10.62 NYY121 pKa = 10.04 EE122 pKa = 4.18 MQNVLYY128 pKa = 10.25 DD129 pKa = 4.12 DD130 pKa = 4.5 GGEE133 pKa = 4.26 TEE135 pKa = 5.69 DD136 pKa = 5.14 DD137 pKa = 4.33 LGDD140 pKa = 3.49 VLYY143 pKa = 11.27 NDD145 pKa = 4.55 GEE147 pKa = 4.64 PNVFLDD153 pKa = 3.62
Molecular weight: 17.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.745
ProMoST 4.05
Dawson 3.923
Bjellqvist 4.101
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.062
Patrickios 1.138
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|A0A514CSU7|A0A514CSU7_9CAUD Polynucleotide kinase OS=Achromobacter phage Motura OX=2591403 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.07 SKK4 pKa = 10.83 RR5 pKa = 11.84 DD6 pKa = 3.27 ISILNKK12 pKa = 10.08 QMNDD16 pKa = 2.96 VEE18 pKa = 4.57 KK19 pKa = 10.91 VLRR22 pKa = 11.84 LPKK25 pKa = 10.21 QFNHH29 pKa = 6.04 WLKK32 pKa = 10.83 QEE34 pKa = 4.0 GLTEE38 pKa = 3.73 EE39 pKa = 4.27 RR40 pKa = 11.84 FRR42 pKa = 11.84 GFRR45 pKa = 11.84 RR46 pKa = 11.84 GHH48 pKa = 3.89 NHH50 pKa = 3.72 YY51 pKa = 9.14 WKK53 pKa = 10.7 GRR55 pKa = 11.84 GRR57 pKa = 11.84 YY58 pKa = 7.35 WRR60 pKa = 11.84 INCFGVFEE68 pKa = 4.29 CSEE71 pKa = 4.12 RR72 pKa = 11.84 FDD74 pKa = 4.71 TFDD77 pKa = 2.75 RR78 pKa = 11.84 WANSNPQVGVSIEE91 pKa = 3.78 WMANRR96 pKa = 11.84 AQFRR100 pKa = 11.84 RR101 pKa = 11.84 AFLQMLRR108 pKa = 11.84 DD109 pKa = 3.68 VRR111 pKa = 11.84 AKK113 pKa = 10.89 GFF115 pKa = 3.48
Molecular weight: 14.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.633
IPC_protein 10.496
Toseland 10.906
ProMoST 10.657
Dawson 10.95
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 11.125
Grimsley 10.994
Solomon 11.14
Lehninger 11.111
Nozaki 10.891
DTASelect 10.687
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.906
Patrickios 10.877
IPC_peptide 11.155
IPC2_peptide 9.75
IPC2.peptide.svr19 8.743
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
344
0
344
71610
31
1528
208.2
23.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.09 ± 0.181
0.947 ± 0.057
5.878 ± 0.197
6.271 ± 0.174
3.803 ± 0.082
6.625 ± 0.173
1.959 ± 0.086
5.044 ± 0.085
6.514 ± 0.274
8.302 ± 0.109
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.673 ± 0.077
4.268 ± 0.098
4.445 ± 0.105
4.376 ± 0.095
4.98 ± 0.116
6.128 ± 0.141
6.413 ± 0.185
7.26 ± 0.13
1.504 ± 0.066
3.522 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here