Amino acid dipepetide frequency for Achromobacter phage Motura

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.594AlaAla: 9.594 ± 0.526
0.712AlaCys: 0.712 ± 0.105
5.055AlaAsp: 5.055 ± 0.276
6.158AlaGlu: 6.158 ± 0.368
3.435AlaPhe: 3.435 ± 0.213
5.893AlaGly: 5.893 ± 0.393
1.871AlaHis: 1.871 ± 0.16
4.106AlaIle: 4.106 ± 0.249
6.745AlaLys: 6.745 ± 0.487
7.471AlaLeu: 7.471 ± 0.351
2.737AlaMet: 2.737 ± 0.179
3.826AlaAsn: 3.826 ± 0.23
3.282AlaPro: 3.282 ± 0.252
4.175AlaGln: 4.175 ± 0.307
4.427AlaArg: 4.427 ± 0.228
5.376AlaSer: 5.376 ± 0.351
4.971AlaThr: 4.971 ± 0.419
6.982AlaVal: 6.982 ± 0.335
1.341AlaTrp: 1.341 ± 0.142
2.737AlaTyr: 2.737 ± 0.205
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.106
0.084CysCys: 0.084 ± 0.035
0.447CysAsp: 0.447 ± 0.083
0.684CysGlu: 0.684 ± 0.093
0.391CysPhe: 0.391 ± 0.067
0.936CysGly: 0.936 ± 0.139
0.293CysHis: 0.293 ± 0.056
0.573CysIle: 0.573 ± 0.095
0.559CysLys: 0.559 ± 0.094
0.573CysLeu: 0.573 ± 0.094
0.14CysMet: 0.14 ± 0.044
0.475CysAsn: 0.475 ± 0.089
0.6CysPro: 0.6 ± 0.116
0.321CysGln: 0.321 ± 0.064
0.475CysArg: 0.475 ± 0.085
0.754CysSer: 0.754 ± 0.104
0.517CysThr: 0.517 ± 0.083
0.517CysVal: 0.517 ± 0.092
0.14CysTrp: 0.14 ± 0.042
0.335CysTyr: 0.335 ± 0.069
0.0CysXaa: 0.0 ± 0.0
Asp
5.293AspAla: 5.293 ± 0.289
0.461AspCys: 0.461 ± 0.082
4.678AspAsp: 4.678 ± 0.913
5.209AspGlu: 5.209 ± 0.606
2.653AspPhe: 2.653 ± 0.186
4.231AspGly: 4.231 ± 0.219
0.81AspHis: 0.81 ± 0.117
2.723AspIle: 2.723 ± 0.196
2.988AspLys: 2.988 ± 0.226
4.832AspLeu: 4.832 ± 0.297
1.201AspMet: 1.201 ± 0.141
2.318AspAsn: 2.318 ± 0.211
3.1AspPro: 3.1 ± 0.224
2.123AspGln: 2.123 ± 0.158
2.402AspArg: 2.402 ± 0.193
3.603AspSer: 3.603 ± 0.281
2.988AspThr: 2.988 ± 0.189
3.854AspVal: 3.854 ± 0.194
1.005AspTrp: 1.005 ± 0.122
2.304AspTyr: 2.304 ± 0.197
0.0AspXaa: 0.0 ± 0.0
Glu
5.851GluAla: 5.851 ± 0.386
0.866GluCys: 0.866 ± 0.109
4.511GluAsp: 4.511 ± 0.564
3.812GluGlu: 3.812 ± 0.303
2.695GluPhe: 2.695 ± 0.171
3.365GluGly: 3.365 ± 0.194
1.452GluHis: 1.452 ± 0.128
3.128GluIle: 3.128 ± 0.215
3.463GluLys: 3.463 ± 0.304
6.2GluLeu: 6.2 ± 0.323
1.355GluMet: 1.355 ± 0.15
1.787GluAsn: 1.787 ± 0.186
2.402GluPro: 2.402 ± 0.216
2.974GluGln: 2.974 ± 0.233
3.812GluArg: 3.812 ± 0.24
3.603GluSer: 3.603 ± 0.238
3.226GluThr: 3.226 ± 0.238
4.692GluVal: 4.692 ± 0.238
1.075GluTrp: 1.075 ± 0.124
2.444GluTyr: 2.444 ± 0.217
0.0GluXaa: 0.0 ± 0.0
Phe
3.142PheAla: 3.142 ± 0.201
0.489PheCys: 0.489 ± 0.085
2.57PheAsp: 2.57 ± 0.177
2.053PheGlu: 2.053 ± 0.177
1.48PhePhe: 1.48 ± 0.157
2.5PheGly: 2.5 ± 0.234
0.866PheHis: 0.866 ± 0.119
1.843PheIle: 1.843 ± 0.158
2.765PheLys: 2.765 ± 0.209
3.114PheLeu: 3.114 ± 0.219
1.033PheMet: 1.033 ± 0.118
1.885PheAsn: 1.885 ± 0.147
1.438PhePro: 1.438 ± 0.132
1.55PheGln: 1.55 ± 0.138
1.941PheArg: 1.941 ± 0.148
2.374PheSer: 2.374 ± 0.194
2.639PheThr: 2.639 ± 0.186
2.514PheVal: 2.514 ± 0.195
0.377PheTrp: 0.377 ± 0.079
1.452PheTyr: 1.452 ± 0.133
0.0PheXaa: 0.0 ± 0.0
Gly
5.516GlyAla: 5.516 ± 0.38
0.796GlyCys: 0.796 ± 0.092
3.812GlyAsp: 3.812 ± 0.258
3.519GlyGlu: 3.519 ± 0.257
3.044GlyPhe: 3.044 ± 0.221
5.041GlyGly: 5.041 ± 0.449
1.117GlyHis: 1.117 ± 0.107
3.729GlyIle: 3.729 ± 0.233
4.385GlyLys: 4.385 ± 0.38
5.293GlyLeu: 5.293 ± 0.345
1.801GlyMet: 1.801 ± 0.16
2.961GlyAsn: 2.961 ± 0.217
2.039GlyPro: 2.039 ± 0.185
2.597GlyGln: 2.597 ± 0.214
2.807GlyArg: 2.807 ± 0.202
3.743GlySer: 3.743 ± 0.276
5.237GlyThr: 5.237 ± 0.433
5.209GlyVal: 5.209 ± 0.339
0.991GlyTrp: 0.991 ± 0.118
2.611GlyTyr: 2.611 ± 0.201
0.0GlyXaa: 0.0 ± 0.0
His
1.41HisAla: 1.41 ± 0.133
0.265HisCys: 0.265 ± 0.061
1.061HisAsp: 1.061 ± 0.148
1.257HisGlu: 1.257 ± 0.151
0.838HisPhe: 0.838 ± 0.109
1.383HisGly: 1.383 ± 0.145
0.433HisHis: 0.433 ± 0.099
0.95HisIle: 0.95 ± 0.128
1.173HisLys: 1.173 ± 0.155
1.871HisLeu: 1.871 ± 0.161
0.74HisMet: 0.74 ± 0.096
0.712HisAsn: 0.712 ± 0.094
0.95HisPro: 0.95 ± 0.119
0.824HisGln: 0.824 ± 0.101
1.019HisArg: 1.019 ± 0.125
1.369HisSer: 1.369 ± 0.131
1.117HisThr: 1.117 ± 0.119
1.285HisVal: 1.285 ± 0.138
0.209HisTrp: 0.209 ± 0.054
0.726HisTyr: 0.726 ± 0.102
0.0HisXaa: 0.0 ± 0.0
Ile
4.566IleAla: 4.566 ± 0.296
0.545IleCys: 0.545 ± 0.094
3.156IleAsp: 3.156 ± 0.24
3.784IleGlu: 3.784 ± 0.233
1.341IlePhe: 1.341 ± 0.115
3.03IleGly: 3.03 ± 0.187
1.033IleHis: 1.033 ± 0.135
2.22IleIle: 2.22 ± 0.199
3.812IleLys: 3.812 ± 0.297
3.421IleLeu: 3.421 ± 0.254
1.215IleMet: 1.215 ± 0.137
2.276IleAsn: 2.276 ± 0.183
2.611IlePro: 2.611 ± 0.191
2.304IleGln: 2.304 ± 0.225
2.458IleArg: 2.458 ± 0.201
3.044IleSer: 3.044 ± 0.199
3.784IleThr: 3.784 ± 0.247
3.715IleVal: 3.715 ± 0.221
0.642IleTrp: 0.642 ± 0.098
1.48IleTyr: 1.48 ± 0.15
0.0IleXaa: 0.0 ± 0.0
Lys
6.801LysAla: 6.801 ± 0.511
0.461LysCys: 0.461 ± 0.088
3.31LysAsp: 3.31 ± 0.258
3.868LysGlu: 3.868 ± 0.329
2.43LysPhe: 2.43 ± 0.195
3.687LysGly: 3.687 ± 0.315
1.396LysHis: 1.396 ± 0.161
2.988LysIle: 2.988 ± 0.228
5.837LysLys: 5.837 ± 0.612
5.502LysLeu: 5.502 ± 0.406
1.941LysMet: 1.941 ± 0.192
2.248LysAsn: 2.248 ± 0.186
3.058LysPro: 3.058 ± 0.284
2.779LysGln: 2.779 ± 0.194
3.282LysArg: 3.282 ± 0.225
4.092LysSer: 4.092 ± 0.418
3.84LysThr: 3.84 ± 0.251
4.092LysVal: 4.092 ± 0.304
1.047LysTrp: 1.047 ± 0.132
2.486LysTyr: 2.486 ± 0.203
0.0LysXaa: 0.0 ± 0.0
Leu
6.857LeuAla: 6.857 ± 0.27
0.782LeuCys: 0.782 ± 0.098
4.874LeuAsp: 4.874 ± 0.248
5.698LeuGlu: 5.698 ± 0.324
2.597LeuPhe: 2.597 ± 0.201
5.39LeuGly: 5.39 ± 0.265
1.606LeuHis: 1.606 ± 0.157
4.217LeuIle: 4.217 ± 0.287
5.781LeuLys: 5.781 ± 0.41
6.382LeuLeu: 6.382 ± 0.33
2.332LeuMet: 2.332 ± 0.206
3.98LeuAsn: 3.98 ± 0.305
4.203LeuPro: 4.203 ± 0.224
4.525LeuGln: 4.525 ± 0.263
4.455LeuArg: 4.455 ± 0.252
4.86LeuSer: 4.86 ± 0.229
4.999LeuThr: 4.999 ± 0.264
5.558LeuVal: 5.558 ± 0.308
0.978LeuTrp: 0.978 ± 0.116
2.947LeuTyr: 2.947 ± 0.208
0.0LeuXaa: 0.0 ± 0.0
Met
2.542MetAla: 2.542 ± 0.185
0.307MetCys: 0.307 ± 0.068
1.452MetAsp: 1.452 ± 0.147
1.508MetGlu: 1.508 ± 0.142
0.978MetPhe: 0.978 ± 0.138
1.229MetGly: 1.229 ± 0.123
0.531MetHis: 0.531 ± 0.085
1.131MetIle: 1.131 ± 0.132
1.76MetLys: 1.76 ± 0.164
2.151MetLeu: 2.151 ± 0.17
0.67MetMet: 0.67 ± 0.094
1.299MetAsn: 1.299 ± 0.133
1.313MetPro: 1.313 ± 0.138
1.327MetGln: 1.327 ± 0.15
1.424MetArg: 1.424 ± 0.133
1.913MetSer: 1.913 ± 0.172
2.178MetThr: 2.178 ± 0.162
1.787MetVal: 1.787 ± 0.155
0.293MetTrp: 0.293 ± 0.065
0.936MetTyr: 0.936 ± 0.105
0.0MetXaa: 0.0 ± 0.0
Asn
3.715AsnAla: 3.715 ± 0.268
0.475AsnCys: 0.475 ± 0.073
2.318AsnAsp: 2.318 ± 0.187
2.276AsnGlu: 2.276 ± 0.187
1.438AsnPhe: 1.438 ± 0.122
3.798AsnGly: 3.798 ± 0.311
0.698AsnHis: 0.698 ± 0.098
1.787AsnIle: 1.787 ± 0.166
2.43AsnLys: 2.43 ± 0.198
3.952AsnLeu: 3.952 ± 0.25
1.075AsnMet: 1.075 ± 0.124
1.592AsnAsn: 1.592 ± 0.133
2.611AsnPro: 2.611 ± 0.197
1.648AsnGln: 1.648 ± 0.15
2.053AsnArg: 2.053 ± 0.194
2.947AsnSer: 2.947 ± 0.209
2.891AsnThr: 2.891 ± 0.221
2.947AsnVal: 2.947 ± 0.24
0.642AsnTrp: 0.642 ± 0.083
1.383AsnTyr: 1.383 ± 0.145
0.0AsnXaa: 0.0 ± 0.0
Pro
4.008ProAla: 4.008 ± 0.26
0.209ProCys: 0.209 ± 0.053
2.639ProAsp: 2.639 ± 0.188
3.114ProGlu: 3.114 ± 0.215
1.718ProPhe: 1.718 ± 0.143
3.198ProGly: 3.198 ± 0.216
0.782ProHis: 0.782 ± 0.103
2.458ProIle: 2.458 ± 0.204
3.002ProLys: 3.002 ± 0.274
2.919ProLeu: 2.919 ± 0.217
1.117ProMet: 1.117 ± 0.144
2.178ProAsn: 2.178 ± 0.199
1.55ProPro: 1.55 ± 0.145
1.927ProGln: 1.927 ± 0.175
1.662ProArg: 1.662 ± 0.171
2.793ProSer: 2.793 ± 0.186
3.226ProThr: 3.226 ± 0.288
3.952ProVal: 3.952 ± 0.228
0.587ProTrp: 0.587 ± 0.08
1.41ProTyr: 1.41 ± 0.136
0.0ProXaa: 0.0 ± 0.0
Gln
4.692GlnAla: 4.692 ± 0.234
0.405GlnCys: 0.405 ± 0.078
1.997GlnAsp: 1.997 ± 0.197
2.178GlnGlu: 2.178 ± 0.187
1.662GlnPhe: 1.662 ± 0.17
3.002GlnGly: 3.002 ± 0.213
0.908GlnHis: 0.908 ± 0.139
2.123GlnIle: 2.123 ± 0.175
2.206GlnLys: 2.206 ± 0.186
3.715GlnLeu: 3.715 ± 0.262
1.48GlnMet: 1.48 ± 0.14
1.48GlnAsn: 1.48 ± 0.139
1.732GlnPro: 1.732 ± 0.143
2.514GlnGln: 2.514 ± 0.214
2.835GlnArg: 2.835 ± 0.215
2.597GlnSer: 2.597 ± 0.204
2.346GlnThr: 2.346 ± 0.18
3.254GlnVal: 3.254 ± 0.206
1.075GlnTrp: 1.075 ± 0.13
1.564GlnTyr: 1.564 ± 0.149
0.0GlnXaa: 0.0 ± 0.0
Arg
4.678ArgAla: 4.678 ± 0.291
0.405ArgCys: 0.405 ± 0.068
2.919ArgAsp: 2.919 ± 0.218
3.226ArgGlu: 3.226 ± 0.271
1.927ArgPhe: 1.927 ± 0.151
3.058ArgGly: 3.058 ± 0.189
0.894ArgHis: 0.894 ± 0.111
2.933ArgIle: 2.933 ± 0.214
3.254ArgLys: 3.254 ± 0.292
4.539ArgLeu: 4.539 ± 0.274
1.494ArgMet: 1.494 ± 0.141
2.388ArgAsn: 2.388 ± 0.201
1.746ArgPro: 1.746 ± 0.152
2.248ArgGln: 2.248 ± 0.18
2.751ArgArg: 2.751 ± 0.208
2.625ArgSer: 2.625 ± 0.209
2.891ArgThr: 2.891 ± 0.244
3.491ArgVal: 3.491 ± 0.215
0.587ArgTrp: 0.587 ± 0.091
1.732ArgTyr: 1.732 ± 0.166
0.0ArgXaa: 0.0 ± 0.0
Ser
5.516SerAla: 5.516 ± 0.394
0.405SerCys: 0.405 ± 0.073
4.161SerAsp: 4.161 ± 0.295
3.617SerGlu: 3.617 ± 0.236
2.276SerPhe: 2.276 ± 0.151
4.539SerGly: 4.539 ± 0.308
1.047SerHis: 1.047 ± 0.137
3.687SerIle: 3.687 ± 0.22
4.134SerLys: 4.134 ± 0.39
4.608SerLeu: 4.608 ± 0.241
1.648SerMet: 1.648 ± 0.14
2.611SerAsn: 2.611 ± 0.204
2.667SerPro: 2.667 ± 0.183
2.36SerGln: 2.36 ± 0.194
2.5SerArg: 2.5 ± 0.227
3.854SerSer: 3.854 ± 0.282
3.743SerThr: 3.743 ± 0.259
4.818SerVal: 4.818 ± 0.284
0.991SerTrp: 0.991 ± 0.113
2.095SerTyr: 2.095 ± 0.156
0.0SerXaa: 0.0 ± 0.0
Thr
5.893ThrAla: 5.893 ± 0.383
0.503ThrCys: 0.503 ± 0.085
3.254ThrAsp: 3.254 ± 0.225
3.352ThrGlu: 3.352 ± 0.234
2.29ThrPhe: 2.29 ± 0.218
4.553ThrGly: 4.553 ± 0.414
1.243ThrHis: 1.243 ± 0.13
3.352ThrIle: 3.352 ± 0.245
3.868ThrLys: 3.868 ± 0.276
6.075ThrLeu: 6.075 ± 0.316
1.466ThrMet: 1.466 ± 0.155
2.947ThrAsn: 2.947 ± 0.288
3.812ThrPro: 3.812 ± 0.26
2.304ThrGln: 2.304 ± 0.218
2.807ThrArg: 2.807 ± 0.164
3.784ThrSer: 3.784 ± 0.278
4.622ThrThr: 4.622 ± 0.488
4.72ThrVal: 4.72 ± 0.275
1.005ThrTrp: 1.005 ± 0.142
2.276ThrTyr: 2.276 ± 0.184
0.0ThrXaa: 0.0 ± 0.0
Val
6.27ValAla: 6.27 ± 0.335
0.614ValCys: 0.614 ± 0.102
3.98ValAsp: 3.98 ± 0.231
4.539ValGlu: 4.539 ± 0.256
2.751ValPhe: 2.751 ± 0.181
4.161ValGly: 4.161 ± 0.285
1.522ValHis: 1.522 ± 0.141
4.008ValIle: 4.008 ± 0.224
4.064ValLys: 4.064 ± 0.263
5.879ValLeu: 5.879 ± 0.279
1.704ValMet: 1.704 ± 0.15
3.589ValAsn: 3.589 ± 0.233
3.407ValPro: 3.407 ± 0.22
3.184ValGln: 3.184 ± 0.239
3.91ValArg: 3.91 ± 0.231
4.329ValSer: 4.329 ± 0.258
5.46ValThr: 5.46 ± 0.331
5.474ValVal: 5.474 ± 0.249
1.089ValTrp: 1.089 ± 0.121
2.667ValTyr: 2.667 ± 0.185
0.0ValXaa: 0.0 ± 0.0
Trp
0.936TrpAla: 0.936 ± 0.12
0.251TrpCys: 0.251 ± 0.069
0.712TrpAsp: 0.712 ± 0.108
0.852TrpGlu: 0.852 ± 0.104
0.67TrpPhe: 0.67 ± 0.088
0.796TrpGly: 0.796 ± 0.103
0.335TrpHis: 0.335 ± 0.066
0.782TrpIle: 0.782 ± 0.11
0.88TrpLys: 0.88 ± 0.124
1.327TrpLeu: 1.327 ± 0.158
0.6TrpMet: 0.6 ± 0.093
0.726TrpAsn: 0.726 ± 0.092
0.559TrpPro: 0.559 ± 0.103
0.628TrpGln: 0.628 ± 0.082
0.796TrpArg: 0.796 ± 0.104
1.103TrpSer: 1.103 ± 0.141
1.131TrpThr: 1.131 ± 0.131
1.229TrpVal: 1.229 ± 0.125
0.251TrpTrp: 0.251 ± 0.063
0.475TrpTyr: 0.475 ± 0.079
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.961TyrAla: 2.961 ± 0.223
0.433TyrCys: 0.433 ± 0.082
1.871TyrAsp: 1.871 ± 0.157
2.011TyrGlu: 2.011 ± 0.158
1.41TyrPhe: 1.41 ± 0.13
2.458TyrGly: 2.458 ± 0.19
0.754TyrHis: 0.754 ± 0.102
1.704TyrIle: 1.704 ± 0.174
2.039TyrLys: 2.039 ± 0.18
3.198TyrLeu: 3.198 ± 0.24
0.964TyrMet: 0.964 ± 0.113
1.396TyrAsn: 1.396 ± 0.144
1.369TyrPro: 1.369 ± 0.147
1.452TyrGln: 1.452 ± 0.15
1.983TyrArg: 1.983 ± 0.157
2.514TyrSer: 2.514 ± 0.202
2.318TyrThr: 2.318 ± 0.191
2.514TyrVal: 2.514 ± 0.159
0.712TyrTrp: 0.712 ± 0.096
1.159TyrTyr: 1.159 ± 0.157
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 344 proteins (71610 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski