Aspergillus novoparasiticus
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14181 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6EB95|A0A5N6EB95_9EURO Uncharacterized protein OS=Aspergillus novoparasiticus OX=986946 GN=BDV33DRAFT_42236 PE=4 SV=1
MM1 pKa = 7.7 ADD3 pKa = 3.51 EE4 pKa = 5.26 AGSIYY9 pKa = 10.71 DD10 pKa = 3.85 EE11 pKa = 4.95 IEE13 pKa = 4.34 IEE15 pKa = 5.79 DD16 pKa = 3.79 MTFDD20 pKa = 6.24 PITQLYY26 pKa = 9.73 HH27 pKa = 5.63 YY28 pKa = 7.55 PCPCGDD34 pKa = 3.67 RR35 pKa = 11.84 FEE37 pKa = 5.7 IMIDD41 pKa = 3.49 DD42 pKa = 4.19 LRR44 pKa = 11.84 DD45 pKa = 3.58 GEE47 pKa = 4.73 EE48 pKa = 3.84 IAVCPSCSLKK58 pKa = 10.28 IRR60 pKa = 11.84 VIFDD64 pKa = 3.02 VDD66 pKa = 4.13 DD67 pKa = 3.69 LHH69 pKa = 9.25 KK70 pKa = 10.81 DD71 pKa = 3.63 DD72 pKa = 4.29 QQQGPSAVAVQAA84 pKa = 4.09
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A5N6EQQ9|A0A5N6EQQ9_9EURO Uncharacterized protein OS=Aspergillus novoparasiticus OX=986946 GN=BDV33DRAFT_204481 PE=4 SV=1
MM1 pKa = 7.45 NSEE4 pKa = 4.73 FSNKK8 pKa = 9.0 QPLSNLSRR16 pKa = 11.84 NSGWTFLLWSVLYY29 pKa = 10.38 NVLGVFVRR37 pKa = 11.84 KK38 pKa = 9.94 RR39 pKa = 11.84 EE40 pKa = 4.07 CFRR43 pKa = 11.84 LRR45 pKa = 11.84 FRR47 pKa = 11.84 GRR49 pKa = 11.84 VPSEE53 pKa = 3.45 DD54 pKa = 3.32 HH55 pKa = 6.6 PRR57 pKa = 11.84 RR58 pKa = 11.84 SCSSDD63 pKa = 2.92 NAKK66 pKa = 10.61 GGWVLGPHH74 pKa = 6.03 GRR76 pKa = 11.84 SLSTRR81 pKa = 11.84 DD82 pKa = 3.64 SYY84 pKa = 11.93 CHH86 pKa = 5.02 VV87 pKa = 3.64
Molecular weight: 10.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.575
IPC_protein 10.526
Toseland 10.613
ProMoST 10.394
Dawson 10.716
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.804
Grimsley 10.774
Solomon 10.862
Lehninger 10.833
Nozaki 10.643
DTASelect 10.467
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.877
IPC2_peptide 9.853
IPC2.peptide.svr19 8.502
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14181
0
14181
6421159
49
7742
452.8
50.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.167 ± 0.017
1.383 ± 0.008
5.576 ± 0.013
6.028 ± 0.021
3.859 ± 0.011
6.797 ± 0.019
2.465 ± 0.009
5.194 ± 0.016
4.562 ± 0.016
9.244 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.172 ± 0.007
3.729 ± 0.009
5.838 ± 0.024
4.003 ± 0.014
5.99 ± 0.019
8.259 ± 0.022
5.944 ± 0.013
6.264 ± 0.017
1.546 ± 0.007
2.979 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here