Perkinsus marinus (strain ATCC 50983 / TXsc)
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23114 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5LP84|C5LP84_PERM5 Transposable element tc3 transposase putative OS=Perkinsus marinus (strain ATCC 50983 / TXsc) OX=423536 GN=Pmar_PMAR009612 PE=4 SV=1
MM1 pKa = 7.72 SSTLDD6 pKa = 3.26 YY7 pKa = 11.14 SAGVNPLACDD17 pKa = 3.97 YY18 pKa = 11.43 DD19 pKa = 3.73 WLADD23 pKa = 3.57 LTLPEE28 pKa = 4.45 FQCGFPEE35 pKa = 5.26 GSDD38 pKa = 3.27 DD39 pKa = 4.15 SSWMDD44 pKa = 3.17 THH46 pKa = 7.02 PCDD49 pKa = 4.95 SPLSPSTTASEE60 pKa = 4.49 VSSLDD65 pKa = 3.25 TALLIQQLLAASSSADD81 pKa = 2.76 IAAAHH86 pKa = 6.32 MSQCEE91 pKa = 4.02 DD92 pKa = 3.72 VEE94 pKa = 4.55 TGATDD99 pKa = 5.18 GISVRR104 pKa = 11.84 KK105 pKa = 9.84 VLDD108 pKa = 3.19 VLIARR113 pKa = 11.84 KK114 pKa = 9.7 GLL116 pKa = 3.57
Molecular weight: 12.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.49
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|C5KKC1|C5KKC1_PERM5 Oxidoreductase putative OS=Perkinsus marinus (strain ATCC 50983 / TXsc) OX=423536 GN=Pmar_PMAR023357 PE=4 SV=1
MM1 pKa = 7.54 CCSEE5 pKa = 4.89 NIGSTAARR13 pKa = 11.84 TWNIRR18 pKa = 11.84 PTAHH22 pKa = 6.77 AGIFVIHH29 pKa = 7.19 AIATTGYY36 pKa = 9.6 RR37 pKa = 11.84 IAEE40 pKa = 4.28 IVTHH44 pKa = 7.13 EE45 pKa = 3.88 ITTISRR51 pKa = 11.84 CCGTRR56 pKa = 11.84 SGNCRR61 pKa = 11.84 TRR63 pKa = 11.84 SSARR67 pKa = 11.84 SSSRR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 TRR75 pKa = 11.84 SSARR79 pKa = 11.84 SSRR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 TRR87 pKa = 11.84 SSARR91 pKa = 11.84 SSRR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 TRR98 pKa = 11.84 SSARR102 pKa = 11.84 CSRR105 pKa = 11.84 RR106 pKa = 11.84 RR107 pKa = 11.84 GRR109 pKa = 11.84 TRR111 pKa = 11.84 SSARR115 pKa = 11.84 SSGGRR120 pKa = 11.84 RR121 pKa = 11.84 GRR123 pKa = 11.84 TRR125 pKa = 11.84 SSARR129 pKa = 11.84 SSGGRR134 pKa = 11.84 RR135 pKa = 11.84 RR136 pKa = 11.84 TRR138 pKa = 11.84 SSARR142 pKa = 11.84 SSGGRR147 pKa = 11.84 RR148 pKa = 11.84 RR149 pKa = 11.84 TRR151 pKa = 11.84 SSARR155 pKa = 11.84 SSGGRR160 pKa = 11.84 RR161 pKa = 11.84 RR162 pKa = 11.84 TRR164 pKa = 11.84 SSARR168 pKa = 11.84 CSRR171 pKa = 11.84 RR172 pKa = 11.84 RR173 pKa = 11.84 GRR175 pKa = 11.84 TRR177 pKa = 11.84 SSARR181 pKa = 11.84 CSRR184 pKa = 11.84 RR185 pKa = 11.84 RR186 pKa = 11.84 GRR188 pKa = 11.84 TRR190 pKa = 11.84 SSARR194 pKa = 11.84 SSRR197 pKa = 11.84 RR198 pKa = 11.84 RR199 pKa = 11.84 GRR201 pKa = 11.84 TRR203 pKa = 11.84 SSARR207 pKa = 11.84 CSGGRR212 pKa = 11.84 RR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 SSARR220 pKa = 11.84 CSRR223 pKa = 11.84 RR224 pKa = 11.84 RR225 pKa = 11.84 GRR227 pKa = 11.84 TRR229 pKa = 11.84 SSARR233 pKa = 11.84 SSGGRR238 pKa = 11.84 RR239 pKa = 11.84 RR240 pKa = 11.84 TRR242 pKa = 11.84 SSARR246 pKa = 11.84 SSGGRR251 pKa = 11.84 RR252 pKa = 11.84 RR253 pKa = 11.84 TRR255 pKa = 11.84 SSARR259 pKa = 11.84 SSGGRR264 pKa = 11.84 RR265 pKa = 11.84 GRR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 TRR271 pKa = 11.84 SSARR275 pKa = 11.84 SSGGRR280 pKa = 11.84 RR281 pKa = 11.84 GRR283 pKa = 11.84 RR284 pKa = 11.84 RR285 pKa = 11.84 TRR287 pKa = 11.84 SSARR291 pKa = 11.84 SSGGRR296 pKa = 11.84 RR297 pKa = 11.84 GRR299 pKa = 11.84 RR300 pKa = 11.84 RR301 pKa = 11.84 TRR303 pKa = 11.84 RR304 pKa = 11.84 AKK306 pKa = 9.55 SSRR309 pKa = 11.84 RR310 pKa = 11.84 RR311 pKa = 11.84 TRR313 pKa = 11.84 RR314 pKa = 11.84 RR315 pKa = 11.84 TRR317 pKa = 11.84 SSARR321 pKa = 11.84 SSRR324 pKa = 11.84 RR325 pKa = 11.84 RR326 pKa = 11.84 TRR328 pKa = 11.84 SSARR332 pKa = 11.84 SSGGRR337 pKa = 11.84 RR338 pKa = 11.84 GRR340 pKa = 11.84 RR341 pKa = 11.84 RR342 pKa = 11.84 TRR344 pKa = 11.84 RR345 pKa = 11.84 AKK347 pKa = 9.55 SSRR350 pKa = 11.84 RR351 pKa = 11.84 RR352 pKa = 11.84 TRR354 pKa = 11.84 RR355 pKa = 11.84 RR356 pKa = 11.84 TRR358 pKa = 11.84 SSARR362 pKa = 11.84 SSRR365 pKa = 11.84 RR366 pKa = 11.84 RR367 pKa = 11.84 TRR369 pKa = 11.84 SSARR373 pKa = 11.84 SSARR377 pKa = 11.84 NSRR380 pKa = 11.84 RR381 pKa = 11.84 RR382 pKa = 11.84 TRR384 pKa = 11.84 SSRR387 pKa = 11.84 RR388 pKa = 11.84 RR389 pKa = 11.84 TRR391 pKa = 11.84 SNRR394 pKa = 11.84 RR395 pKa = 11.84 RR396 pKa = 11.84 TRR398 pKa = 11.84 SSRR401 pKa = 11.84 RR402 pKa = 11.84 RR403 pKa = 11.84 TRR405 pKa = 11.84 SSRR408 pKa = 11.84 RR409 pKa = 11.84 RR410 pKa = 11.84 TRR412 pKa = 11.84 SSRR415 pKa = 11.84 RR416 pKa = 11.84 RR417 pKa = 11.84 TRR419 pKa = 11.84 SSRR422 pKa = 11.84 RR423 pKa = 11.84 RR424 pKa = 11.84 SARR427 pKa = 11.84 SSRR430 pKa = 11.84 RR431 pKa = 11.84 RR432 pKa = 11.84 TRR434 pKa = 11.84 RR435 pKa = 11.84 RR436 pKa = 11.84 TRR438 pKa = 11.84 SSARR442 pKa = 11.84 SSRR445 pKa = 11.84 HH446 pKa = 4.01 RR447 pKa = 11.84 RR448 pKa = 11.84 TRR450 pKa = 11.84 SGMSMRR456 pKa = 11.84 APGIIPLYY464 pKa = 10.56 KK465 pKa = 10.35 SITSFSVIPYY475 pKa = 10.56 DD476 pKa = 5.2 KK477 pKa = 10.19 ITT479 pKa = 3.41
Molecular weight: 55.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.242
IPC_protein 12.808
Toseland 12.969
ProMoST 13.466
Dawson 12.969
Bjellqvist 12.969
Wikipedia 13.451
Rodwell 12.471
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.969
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.179
IPC_peptide 13.466
IPC2_peptide 12.457
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22958
156
23114
7493757
15
7897
324.2
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.411 ± 0.014
1.891 ± 0.009
5.976 ± 0.013
6.717 ± 0.02
3.383 ± 0.009
7.02 ± 0.016
2.245 ± 0.009
4.702 ± 0.013
5.054 ± 0.018
8.984 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.008
3.298 ± 0.011
5.038 ± 0.014
3.507 ± 0.01
6.256 ± 0.018
7.745 ± 0.019
5.744 ± 0.024
7.285 ± 0.014
1.318 ± 0.007
2.793 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here