Avon-Heathcote Estuary associated circular virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBF7|A0A0C5IBF7_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 1 OX=1618232 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 9.89TRR4 pKa = 11.84LWCGTQYY11 pKa = 11.73DD12 pKa = 4.01LGFNFEE18 pKa = 4.61KK19 pKa = 10.89LVDD22 pKa = 4.24DD23 pKa = 5.75EE24 pKa = 4.35ILTYY28 pKa = 9.85VAWGDD33 pKa = 3.91EE34 pKa = 4.37VCPTTEE40 pKa = 4.09RR41 pKa = 11.84KK42 pKa = 9.37HH43 pKa = 5.32KK44 pKa = 10.37QFWCMFKK51 pKa = 10.59SPRR54 pKa = 11.84GSMKK58 pKa = 10.28QVGKK62 pKa = 10.96VFTSWPNKK70 pKa = 8.72GMQGKK75 pKa = 8.27VFPCSGSIKK84 pKa = 10.79SNEE87 pKa = 4.12EE88 pKa = 3.67YY89 pKa = 10.44CKK91 pKa = 10.8KK92 pKa = 10.02EE93 pKa = 3.91GSYY96 pKa = 10.63HH97 pKa = 6.95KK98 pKa = 10.45YY99 pKa = 10.23GKK101 pKa = 10.18EE102 pKa = 3.69PEE104 pKa = 3.86QGKK107 pKa = 10.46RR108 pKa = 11.84NDD110 pKa = 4.03IIGCMKK116 pKa = 9.66MVKK119 pKa = 9.91EE120 pKa = 4.27GAAEE124 pKa = 3.97LEE126 pKa = 4.13IAEE129 pKa = 4.46TSPHH133 pKa = 6.48LWCQYY138 pKa = 8.31GRR140 pKa = 11.84RR141 pKa = 11.84FEE143 pKa = 4.28EE144 pKa = 4.15YY145 pKa = 9.98RR146 pKa = 11.84ALLQPDD152 pKa = 4.09RR153 pKa = 11.84DD154 pKa = 3.61WVPEE158 pKa = 3.98VKK160 pKa = 10.29VWWGEE165 pKa = 3.81PGSGKK170 pKa = 10.15SRR172 pKa = 11.84AARR175 pKa = 11.84EE176 pKa = 3.78WLGEE180 pKa = 3.95YY181 pKa = 9.95DD182 pKa = 3.66VCTYY186 pKa = 9.52TAGGFFIGYY195 pKa = 9.05KK196 pKa = 8.51NHH198 pKa = 6.45EE199 pKa = 4.62SVLLEE204 pKa = 4.42DD205 pKa = 5.19FDD207 pKa = 4.79FKK209 pKa = 11.68SMPRR213 pKa = 11.84DD214 pKa = 3.34VFLQCTDD221 pKa = 3.19RR222 pKa = 11.84YY223 pKa = 9.53KK224 pKa = 10.92HH225 pKa = 5.44VVNVKK230 pKa = 10.03NGEE233 pKa = 4.31RR234 pKa = 11.84NWNPRR239 pKa = 11.84RR240 pKa = 11.84IAITSNFDD248 pKa = 3.21PEE250 pKa = 4.3EE251 pKa = 4.2WYY253 pKa = 10.2KK254 pKa = 10.74WGDD257 pKa = 3.39PKK259 pKa = 11.27AVTRR263 pKa = 11.84RR264 pKa = 11.84ITSITKK270 pKa = 9.14MEE272 pKa = 4.42HH273 pKa = 5.77KK274 pKa = 10.63

Molecular weight: 32.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBF7|A0A0C5IBF7_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 1 OX=1618232 PE=4 SV=1
MM1 pKa = 7.75PLGRR5 pKa = 11.84LHH7 pKa = 7.66PYY9 pKa = 10.17RR10 pKa = 11.84RR11 pKa = 11.84GTAARR16 pKa = 11.84KK17 pKa = 8.32IQAAFRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84TKK28 pKa = 10.63KK29 pKa = 9.68RR30 pKa = 11.84FQRR33 pKa = 11.84DD34 pKa = 3.47KK35 pKa = 10.75IKK37 pKa = 9.73TAGDD41 pKa = 3.08VRR43 pKa = 11.84RR44 pKa = 11.84AVQQTSVSSVVVQEE58 pKa = 4.27TQAQGLSTTPTVIHH72 pKa = 6.33NLSKK76 pKa = 10.71IEE78 pKa = 4.13FNNSNDD84 pKa = 3.04TLYY87 pKa = 11.07ARR89 pKa = 11.84KK90 pKa = 9.84SKK92 pKa = 10.69QITVGHH98 pKa = 6.01FKK100 pKa = 10.37FRR102 pKa = 11.84AKK104 pKa = 10.89VNVADD109 pKa = 3.74KK110 pKa = 10.46TNLIRR115 pKa = 11.84IMVVRR120 pKa = 11.84NKK122 pKa = 10.91DD123 pKa = 3.25PATTNAFDD131 pKa = 4.23PADD134 pKa = 3.69MFAMNNSQGLPADD147 pKa = 3.65NMFAEE152 pKa = 4.65PNLRR156 pKa = 11.84NVEE159 pKa = 4.13VKK161 pKa = 10.3YY162 pKa = 11.12DD163 pKa = 3.51RR164 pKa = 11.84VFNLQATSEE173 pKa = 4.48TIPAVNPQSVYY184 pKa = 6.7WTFDD188 pKa = 3.12VPVRR192 pKa = 11.84EE193 pKa = 4.08TFKK196 pKa = 11.33YY197 pKa = 10.57FGTTNGTSEE206 pKa = 4.0QTRR209 pKa = 11.84NMKK212 pKa = 10.25DD213 pKa = 3.27YY214 pKa = 10.8YY215 pKa = 11.41LLGFSDD221 pKa = 4.15SSLITHH227 pKa = 6.73PTIQVVSYY235 pKa = 10.27VWFKK239 pKa = 11.47NVGNTAA245 pKa = 3.19

Molecular weight: 27.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

519

245

274

259.5

30.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.588 ± 1.509

1.734 ± 1.207

5.01 ± 0.078

6.166 ± 2.303

5.202 ± 0.356

6.358 ± 1.585

1.927 ± 0.205

3.854 ± 0.159

8.285 ± 1.221

4.624 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.173

5.588 ± 1.509

4.624 ± 0.094

4.046 ± 0.877

7.129 ± 0.72

5.588 ± 0.372

7.322 ± 1.722

7.707 ± 1.17

2.697 ± 1.31

3.854 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski