Fowl aviadenovirus D
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096ZH25|A0A096ZH25_9ADEN dUTP diphosphatase OS=Fowl aviadenovirus D OX=190064 PE=3 SV=1
MM1 pKa = 7.52 AKK3 pKa = 8.76 STPFTFSMGQHH14 pKa = 6.21 SSRR17 pKa = 11.84 KK18 pKa = 9.39 RR19 pKa = 11.84 PADD22 pKa = 3.44 SEE24 pKa = 4.19 NTQNASKK31 pKa = 10.45 VAKK34 pKa = 8.38 TQTSATRR41 pKa = 11.84 AGVDD45 pKa = 3.89 GNDD48 pKa = 4.54 DD49 pKa = 4.1 LNLVYY54 pKa = 9.94 PFWLQNSTSGGGGGGSGGNPSLNPPFIDD82 pKa = 3.73 PNGPLYY88 pKa = 10.31 VQNSLLYY95 pKa = 10.35 VKK97 pKa = 7.76 TTAPIEE103 pKa = 4.16 VEE105 pKa = 4.18 NKK107 pKa = 10.19 SLALAYY113 pKa = 10.24 DD114 pKa = 3.34 SSLDD118 pKa = 3.52 VDD120 pKa = 4.26 AQNQLQVKK128 pKa = 9.2 VDD130 pKa = 3.45 AEE132 pKa = 4.46 GPIRR136 pKa = 11.84 ISPDD140 pKa = 3.08 GLDD143 pKa = 3.18 IAVDD147 pKa = 4.11 PSTLEE152 pKa = 4.49 VDD154 pKa = 4.89 DD155 pKa = 4.35 EE156 pKa = 4.52 WEE158 pKa = 4.23 LTVKK162 pKa = 10.5 LDD164 pKa = 3.59 PAGPISSSSAGINIRR179 pKa = 11.84 VDD181 pKa = 3.33 DD182 pKa = 4.2 TLLIEE187 pKa = 5.93 DD188 pKa = 5.57 DD189 pKa = 3.94 DD190 pKa = 4.28 TAQVKK195 pKa = 9.74 EE196 pKa = 4.6 LGVHH200 pKa = 6.68 LNPNGPITADD210 pKa = 3.16 QDD212 pKa = 4.14 GLDD215 pKa = 4.68 LEE217 pKa = 5.14 VDD219 pKa = 3.83 PQTLTVTTSGATGGVLGVLLKK240 pKa = 10.67 PSGGLQTSIQGIGVAVADD258 pKa = 4.18 TLTISSNTGTVEE270 pKa = 4.52 VKK272 pKa = 9.83 TDD274 pKa = 3.75 PNGSIGSSSNGIAVVTDD291 pKa = 3.45 PAGPLTTSSNGLSLKK306 pKa = 8.58 LTPNGSIQSSSTGLSVQTDD325 pKa = 3.58 PAGPITSGANGLSLSYY341 pKa = 9.5 DD342 pKa = 3.29 TSDD345 pKa = 3.48 FTVSQGMLSIIRR357 pKa = 11.84 NPSAYY362 pKa = 8.95 PDD364 pKa = 3.84 AYY366 pKa = 10.95 LEE368 pKa = 4.65 SGTNLLNNYY377 pKa = 7.05 TAYY380 pKa = 10.66 AEE382 pKa = 4.05 NSSNYY387 pKa = 9.63 KK388 pKa = 9.97 FNCAYY393 pKa = 10.3 FLQSWYY399 pKa = 11.27 SNGLVTSSLYY409 pKa = 11.07 LKK411 pKa = 10.39 INRR414 pKa = 11.84 DD415 pKa = 3.68 NLTSLPSGQLSEE427 pKa = 3.98 NAKK430 pKa = 10.55 YY431 pKa = 8.24 FTFWVPTYY439 pKa = 11.17 EE440 pKa = 5.04 SMNLSNVATPTITPSSVPWGAFLPAQNCTSNPAFKK475 pKa = 10.69 YY476 pKa = 10.75 YY477 pKa = 9.51 LTQPPSIYY485 pKa = 10.32 FEE487 pKa = 4.53 PEE489 pKa = 3.66 SGSVQTFQPVLTGDD503 pKa = 3.49 WDD505 pKa = 4.24 TNTYY509 pKa = 10.85 NPGTVQVCILPQTVVGGQSTFVNMTCYY536 pKa = 10.52 NFRR539 pKa = 11.84 CQNPGIFKK547 pKa = 10.24 VAASSGTFTIGPIFYY562 pKa = 10.31 SCPTNKK568 pKa = 8.58 LTQPP572 pKa = 3.58
Molecular weight: 60.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.879
IPC2_protein 4.126
IPC_protein 4.139
Toseland 3.923
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.05
Rodwell 3.961
Grimsley 3.834
Solomon 4.126
Lehninger 4.075
Nozaki 4.228
DTASelect 4.482
Thurlkill 3.961
EMBOSS 4.062
Sillero 4.253
Patrickios 1.977
IPC_peptide 4.113
IPC2_peptide 4.24
IPC2.peptide.svr19 4.141
Protein with the highest isoelectric point:
>tr|A0A096ZH39|A0A096ZH39_9ADEN Isoform of A0A096ZH27 Pre-hexon-linking protein VIII OS=Fowl aviadenovirus D OX=190064 GN=L4 PE=2 SV=1
MM1 pKa = 8.23 DD2 pKa = 4.06 YY3 pKa = 11.11 AALSPHH9 pKa = 5.83 VGGWALRR16 pKa = 11.84 DD17 pKa = 3.88 GYY19 pKa = 11.25 LGDD22 pKa = 3.42 SSLRR26 pKa = 11.84 GGAINWSNVGSRR38 pKa = 11.84 LSSALGTAGRR48 pKa = 11.84 WMFNQGNRR56 pKa = 11.84 FLNSNTFGQIKK67 pKa = 10.24 QGFKK71 pKa = 10.64 DD72 pKa = 3.17 SGVIRR77 pKa = 11.84 NVANLAGEE85 pKa = 4.3 TLGALTDD92 pKa = 3.46 IGRR95 pKa = 11.84 LKK97 pKa = 10.28 IQQDD101 pKa = 3.8 LEE103 pKa = 4.06 KK104 pKa = 10.67 LRR106 pKa = 11.84 RR107 pKa = 11.84 KK108 pKa = 9.96 ALGEE112 pKa = 4.63 DD113 pKa = 4.23 GPATQAEE120 pKa = 4.55 LQALIQALQAQVAAGEE136 pKa = 4.64 SVPTTAPTVGPSAPAEE152 pKa = 4.31 VPRR155 pKa = 11.84 PTTRR159 pKa = 11.84 PIPEE163 pKa = 4.15 MVTEE167 pKa = 3.93 VRR169 pKa = 11.84 PPVTSSAPAVPEE181 pKa = 3.78 TDD183 pKa = 2.94 RR184 pKa = 11.84 PTTLEE189 pKa = 3.83 MRR191 pKa = 11.84 PPPAKK196 pKa = 9.84 RR197 pKa = 11.84 RR198 pKa = 11.84 RR199 pKa = 11.84 KK200 pKa = 9.85 RR201 pKa = 11.84 PAPGSWRR208 pKa = 11.84 TRR210 pKa = 11.84 LNSLSGNGVAVSRR223 pKa = 11.84 RR224 pKa = 11.84 RR225 pKa = 11.84 MCYY228 pKa = 9.89
Molecular weight: 24.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.663
IPC_protein 10.774
Toseland 10.935
ProMoST 10.979
Dawson 10.994
Bjellqvist 10.804
Wikipedia 11.301
Rodwell 11.008
Grimsley 11.038
Solomon 11.257
Lehninger 11.199
Nozaki 10.921
DTASelect 10.804
Thurlkill 10.935
EMBOSS 11.374
Sillero 10.95
Patrickios 10.745
IPC_peptide 11.272
IPC2_peptide 9.999
IPC2.peptide.svr19 8.844
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
1
19
9142
163
1306
481.2
53.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.412 ± 0.413
1.499 ± 0.241
5.699 ± 0.331
6.147 ± 0.722
4.135 ± 0.236
6.519 ± 0.434
2.428 ± 0.3
3.861 ± 0.127
3.927 ± 0.436
8.127 ± 0.326
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.286 ± 0.201
4.299 ± 0.531
6.651 ± 0.258
3.828 ± 0.314
7.132 ± 0.616
7.001 ± 0.499
6.137 ± 0.419
6.683 ± 0.318
1.258 ± 0.126
3.971 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here