Hubei narna-like virus 21
Average proteome isoelectric point is 9.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIL8|A0A1L3KIL8_9VIRU Uncharacterized protein OS=Hubei narna-like virus 21 OX=1922952 PE=4 SV=1
MM1 pKa = 7.2 GRR3 pKa = 11.84 EE4 pKa = 3.97 EE5 pKa = 3.88 TVKK8 pKa = 10.39 RR9 pKa = 11.84 VKK11 pKa = 10.36 QYY13 pKa = 11.64 AEE15 pKa = 3.8 YY16 pKa = 10.59 CRR18 pKa = 11.84 ARR20 pKa = 11.84 SIDD23 pKa = 3.28 ARR25 pKa = 11.84 QAEE28 pKa = 4.53 AGPPRR33 pKa = 11.84 GFPFHH38 pKa = 6.95 ALEE41 pKa = 4.76 GGSFKK46 pKa = 11.1 ALLRR50 pKa = 11.84 CPVQGPAALAQVARR64 pKa = 11.84 LGRR67 pKa = 11.84 SMPLPTHH74 pKa = 6.13 KK75 pKa = 10.49 QQGRR79 pKa = 11.84 ALIEE83 pKa = 4.14 HH84 pKa = 6.71 CNEE87 pKa = 3.73 LTAGPHH93 pKa = 5.17 EE94 pKa = 5.03 EE95 pKa = 4.37 IPVEE99 pKa = 3.89 LLDD102 pKa = 4.84 AFYY105 pKa = 11.09 YY106 pKa = 7.15 WTRR109 pKa = 11.84 TWGAGKK115 pKa = 9.99 GAALRR120 pKa = 11.84 DD121 pKa = 3.65 EE122 pKa = 4.46 VTVNFSSAAAEE133 pKa = 4.28 SVSRR137 pKa = 11.84 ARR139 pKa = 11.84 GGQRR143 pKa = 11.84 EE144 pKa = 4.27 EE145 pKa = 4.35 LRR147 pKa = 11.84 LLAAPHH153 pKa = 6.26 FEE155 pKa = 5.0 AIRR158 pKa = 11.84 EE159 pKa = 4.06 ALAIDD164 pKa = 5.25 DD165 pKa = 3.19 IDD167 pKa = 3.93 FRR169 pKa = 11.84 VEE171 pKa = 3.59 EE172 pKa = 4.34 GDD174 pKa = 3.52 YY175 pKa = 11.25 AGIYY179 pKa = 9.96 SEE181 pKa = 5.16 EE182 pKa = 4.84 DD183 pKa = 3.16 DD184 pKa = 5.91 LEE186 pKa = 6.46 IITQAAIEE194 pKa = 4.21 RR195 pKa = 11.84 VRR197 pKa = 11.84 ADD199 pKa = 2.57 IRR201 pKa = 11.84 AGRR204 pKa = 11.84 RR205 pKa = 11.84 PDD207 pKa = 2.92 VRR209 pKa = 11.84 AAVVPEE215 pKa = 4.7 LGAKK219 pKa = 9.58 SRR221 pKa = 11.84 IVTAGEE227 pKa = 3.83 AHH229 pKa = 6.23 WVVIGDD235 pKa = 5.38 AIRR238 pKa = 11.84 KK239 pKa = 7.73 CLWPALEE246 pKa = 4.35 AEE248 pKa = 4.25 PRR250 pKa = 11.84 IDD252 pKa = 3.27 LSGRR256 pKa = 11.84 RR257 pKa = 11.84 DD258 pKa = 3.58 VAGTAAHH265 pKa = 6.36 LHH267 pKa = 6.55 DD268 pKa = 5.03 EE269 pKa = 4.37 VAKK272 pKa = 10.78 ALTGAEE278 pKa = 3.67 AVEE281 pKa = 5.55 FYY283 pKa = 11.1 SADD286 pKa = 3.28 LTAATDD292 pKa = 4.14 LMPHH296 pKa = 7.0 DD297 pKa = 6.14 LIQAIWRR304 pKa = 11.84 GLMEE308 pKa = 4.63 GLGVSDD314 pKa = 5.23 DD315 pKa = 4.87 DD316 pKa = 5.53 LMFTAGLALLGPVNLHH332 pKa = 6.81 HH333 pKa = 7.73 PDD335 pKa = 4.22 LDD337 pKa = 4.18 DD338 pKa = 4.92 LDD340 pKa = 5.53 RR341 pKa = 11.84 PGKK344 pKa = 8.52 YY345 pKa = 10.16 AYY347 pKa = 10.14 GVTHH351 pKa = 7.35 KK352 pKa = 10.34 GCMMGFPLSWYY363 pKa = 9.76 ILNLYY368 pKa = 9.93 NLACADD374 pKa = 3.99 LALSAEE380 pKa = 4.29 RR381 pKa = 11.84 GRR383 pKa = 11.84 FLAEE387 pKa = 3.44 VAPRR391 pKa = 11.84 LVGAAPAVVRR401 pKa = 11.84 GDD403 pKa = 4.28 DD404 pKa = 3.66 LCSAHH409 pKa = 6.23 TPAEE413 pKa = 3.78 ADD415 pKa = 3.25 RR416 pKa = 11.84 YY417 pKa = 9.66 EE418 pKa = 4.58 RR419 pKa = 11.84 IIRR422 pKa = 11.84 ATGGRR427 pKa = 11.84 ANLSKK432 pKa = 10.56 SYY434 pKa = 10.5 RR435 pKa = 11.84 SRR437 pKa = 11.84 KK438 pKa = 9.69 GFILAEE444 pKa = 4.03 RR445 pKa = 11.84 TFLVTTARR453 pKa = 11.84 TKK455 pKa = 10.8 CSAARR460 pKa = 11.84 SRR462 pKa = 11.84 LGTVVVRR469 pKa = 11.84 PGVHH473 pKa = 6.08 PRR475 pKa = 11.84 APLLTRR481 pKa = 11.84 APDD484 pKa = 3.58 AFEE487 pKa = 4.83 SGALGYY493 pKa = 10.53 RR494 pKa = 11.84 SIDD497 pKa = 3.09 PRR499 pKa = 11.84 SRR501 pKa = 11.84 RR502 pKa = 11.84 RR503 pKa = 11.84 GAATVVGLTMLSDD516 pKa = 3.65 VPVRR520 pKa = 11.84 HH521 pKa = 6.4 LMPSPDD527 pKa = 3.94 SEE529 pKa = 4.24 GLPAYY534 pKa = 8.35 VTLPPAASAVVDD546 pKa = 4.54 EE547 pKa = 5.01 IKK549 pKa = 10.52 DD550 pKa = 3.37 IKK552 pKa = 10.46 RR553 pKa = 11.84 RR554 pKa = 11.84 EE555 pKa = 4.24 AICYY559 pKa = 9.76 GILSVNAPLVRR570 pKa = 11.84 QFNRR574 pKa = 11.84 FKK576 pKa = 10.92 IPIFYY581 pKa = 9.95 PRR583 pKa = 11.84 EE584 pKa = 3.71 LGGAGFPHH592 pKa = 7.06 PKK594 pKa = 10.38 GFGSAIRR601 pKa = 11.84 SAGGMGHH608 pKa = 7.38 LRR610 pKa = 11.84 ATLAMTTFTRR620 pKa = 11.84 RR621 pKa = 11.84 ARR623 pKa = 11.84 QAYY626 pKa = 9.46 QLDD629 pKa = 4.18 RR630 pKa = 11.84 DD631 pKa = 3.62 PWICDD636 pKa = 3.05 RR637 pKa = 11.84 QVEE640 pKa = 4.64 FEE642 pKa = 4.12 RR643 pKa = 11.84 SRR645 pKa = 11.84 AQTGLIAEE653 pKa = 4.42 EE654 pKa = 3.82 RR655 pKa = 11.84 RR656 pKa = 11.84 AAEE659 pKa = 4.12 SGQLGLATSVPLEE672 pKa = 4.26 DD673 pKa = 4.27 AVAARR678 pKa = 11.84 AAEE681 pKa = 4.24 GQLWAEE687 pKa = 4.13 VMLPQSRR694 pKa = 11.84 EE695 pKa = 3.72 LASARR700 pKa = 11.84 RR701 pKa = 11.84 GRR703 pKa = 11.84 RR704 pKa = 11.84 GPRR707 pKa = 11.84 LSSVRR712 pKa = 11.84 KK713 pKa = 9.52 GLSDD717 pKa = 3.96 VFNALAKK724 pKa = 10.03 VHH726 pKa = 6.71 AKK728 pKa = 10.08 FDD730 pKa = 4.19 PKK732 pKa = 10.62 FLANPRR738 pKa = 11.84 IDD740 pKa = 3.38 KK741 pKa = 7.32 EE742 pKa = 4.25 TWMDD746 pKa = 3.62 RR747 pKa = 11.84 VAMAKK752 pKa = 10.27 GSSTVYY758 pKa = 9.97 RR759 pKa = 11.84 VVRR762 pKa = 11.84 PSGRR766 pKa = 11.84 RR767 pKa = 11.84 VVMHH771 pKa = 7.19 GIRR774 pKa = 11.84 DD775 pKa = 3.56 THH777 pKa = 7.54 RR778 pKa = 11.84 EE779 pKa = 3.47 IRR781 pKa = 11.84 AEE783 pKa = 3.9 RR784 pKa = 11.84 EE785 pKa = 3.78 MLKK788 pKa = 10.82 GGWTFGGQRR797 pKa = 11.84 DD798 pKa = 3.88 LRR800 pKa = 11.84 QNTAAGLEE808 pKa = 4.25 GGEE811 pKa = 4.17 IAVPVPTQRR820 pKa = 11.84 AGATLWDD827 pKa = 4.06 VLSVSCKK834 pKa = 9.19 RR835 pKa = 11.84 ARR837 pKa = 11.84 KK838 pKa = 8.94 RR839 pKa = 11.84 KK840 pKa = 9.47 KK841 pKa = 10.33 GNPRR845 pKa = 11.84 LL846 pKa = 3.73
Molecular weight: 92.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.945
IPC2_protein 8.814
IPC_protein 8.858
Toseland 9.428
ProMoST 9.297
Dawson 9.736
Bjellqvist 9.545
Wikipedia 9.94
Rodwell 9.882
Grimsley 9.809
Solomon 9.78
Lehninger 9.736
Nozaki 9.648
DTASelect 9.472
Thurlkill 9.589
EMBOSS 9.882
Sillero 9.721
Patrickios 5.27
IPC_peptide 9.765
IPC2_peptide 8.404
IPC2.peptide.svr19 7.805
Protein with the highest isoelectric point:
>tr|A0A1L3KIL8|A0A1L3KIL8_9VIRU Uncharacterized protein OS=Hubei narna-like virus 21 OX=1922952 PE=4 SV=1
MM1 pKa = 7.64 LLQAGVAFLPFPSALTAHH19 pKa = 6.37 TEE21 pKa = 4.08 YY22 pKa = 10.49 IPEE25 pKa = 4.52 CSSSSLCRR33 pKa = 11.84 YY34 pKa = 10.14 RR35 pKa = 11.84 NGNLPSFQTCRR46 pKa = 11.84 GVLPQVALAPKK57 pKa = 10.08 CPPTLQHH64 pKa = 6.71 LSLRR68 pKa = 11.84 TDD70 pKa = 4.2 FPVRR74 pKa = 11.84 IPNSMHH80 pKa = 6.59 NDD82 pKa = 2.98 APAARR87 pKa = 11.84 SNNPINGTRR96 pKa = 11.84 TLRR99 pKa = 11.84 HH100 pKa = 6.31 CNAVHH105 pKa = 7.08 PGLLVNPRR113 pKa = 11.84 IGKK116 pKa = 9.15 KK117 pKa = 10.12 LRR119 pKa = 11.84 IEE121 pKa = 4.03 LRR123 pKa = 11.84 VDD125 pKa = 4.13 FSEE128 pKa = 4.69 GVEE131 pKa = 4.27 HH132 pKa = 6.76 VRR134 pKa = 11.84 EE135 pKa = 4.19 PLPHH139 pKa = 6.35 RR140 pKa = 11.84 RR141 pKa = 11.84 EE142 pKa = 3.98 ARR144 pKa = 11.84 TATPAPRR151 pKa = 11.84 GGQLAGLRR159 pKa = 11.84 QHH161 pKa = 6.58 NLGPEE166 pKa = 3.98 LSLRR170 pKa = 11.84 RR171 pKa = 11.84 SRR173 pKa = 11.84 GHH175 pKa = 6.66 RR176 pKa = 11.84 VLQRR180 pKa = 11.84 HH181 pKa = 5.18 RR182 pKa = 11.84 RR183 pKa = 11.84 GEE185 pKa = 4.19 SEE187 pKa = 4.27 LSAFSGAALFRR198 pKa = 11.84 DD199 pKa = 3.94 QSGLRR204 pKa = 11.84 AATFKK209 pKa = 11.2 FNLTVAYY216 pKa = 9.05 PRR218 pKa = 11.84 IAIEE222 pKa = 3.92 LVRR225 pKa = 11.84 LSRR228 pKa = 11.84 SAGEE232 pKa = 3.89 GRR234 pKa = 11.84 HH235 pKa = 5.45 CKK237 pKa = 9.85 RR238 pKa = 11.84 RR239 pKa = 11.84 SQMTHH244 pKa = 4.86 TSRR247 pKa = 11.84 GADD250 pKa = 3.27 GASEE254 pKa = 4.37 SLRR257 pKa = 11.84 MRR259 pKa = 11.84 KK260 pKa = 9.3 PGSPEE265 pKa = 3.3 LSRR268 pKa = 11.84 IEE270 pKa = 4.3 DD271 pKa = 3.56 RR272 pKa = 11.84 NLEE275 pKa = 4.64 PIEE278 pKa = 4.91 LPHH281 pKa = 6.01 QRR283 pKa = 11.84 SVYY286 pKa = 7.05 TQNPITDD293 pKa = 4.47 RR294 pKa = 11.84 LPPLYY299 pKa = 10.18 ILDD302 pKa = 4.15 FVHH305 pKa = 7.18 HH306 pKa = 6.02 STSCRR311 pKa = 11.84 RR312 pKa = 11.84 KK313 pKa = 9.84 RR314 pKa = 11.84 DD315 pKa = 3.28 IRR317 pKa = 11.84 WQPFRR322 pKa = 11.84 VWRR325 pKa = 11.84 RR326 pKa = 11.84 HH327 pKa = 4.43 QMPDD331 pKa = 2.9 RR332 pKa = 11.84 NVAQHH337 pKa = 6.71 RR338 pKa = 11.84 EE339 pKa = 3.69 AHH341 pKa = 5.81 NRR343 pKa = 11.84 GCAPPTAARR352 pKa = 11.84 IDD354 pKa = 3.55 RR355 pKa = 11.84 TVPKK359 pKa = 8.76 GTRR362 pKa = 11.84 FEE364 pKa = 4.6 RR365 pKa = 11.84 IRR367 pKa = 11.84 RR368 pKa = 11.84 ARR370 pKa = 11.84 QQGSPRR376 pKa = 11.84 MNPRR380 pKa = 11.84 SHH382 pKa = 7.72 DD383 pKa = 3.86 DD384 pKa = 3.55 CPQTGSCRR392 pKa = 11.84 GTFGPRR398 pKa = 11.84 CGHH401 pKa = 5.7 QEE403 pKa = 4.0 GPLSKK408 pKa = 10.92 DD409 pKa = 3.26 EE410 pKa = 5.16 AFTTPVRR417 pKa = 11.84 LAEE420 pKa = 4.24 VRR422 pKa = 11.84 SSPCCSDD429 pKa = 3.9 DD430 pKa = 3.46 PLIAVRR436 pKa = 11.84 LCRR439 pKa = 11.84 RR440 pKa = 11.84 VRR442 pKa = 11.84 RR443 pKa = 11.84 AQIISPDD450 pKa = 3.37 NCRR453 pKa = 11.84 SSAHH457 pKa = 5.27 QPRR460 pKa = 11.84 SYY462 pKa = 10.89 LRR464 pKa = 11.84 EE465 pKa = 3.52 KK466 pKa = 10.58 AAALGRR472 pKa = 11.84 QSQVSTGKK480 pKa = 9.94 VVEE483 pKa = 4.42 VKK485 pKa = 10.57 NIPRR489 pKa = 11.84 KK490 pKa = 10.0 RR491 pKa = 11.84 EE492 pKa = 3.63 AHH494 pKa = 6.06 HH495 pKa = 6.2 AAFMGNPIGIFTRR508 pKa = 11.84 TIQIVEE514 pKa = 3.77 VRR516 pKa = 11.84 MMQVHH521 pKa = 6.76 RR522 pKa = 11.84 SEE524 pKa = 4.04 QSKK527 pKa = 9.97 PSCEE531 pKa = 4.08 HH532 pKa = 5.02 QVVVGNAQTFHH543 pKa = 6.64 EE544 pKa = 4.75 PSPNGLNKK552 pKa = 9.64 VMRR555 pKa = 11.84 HH556 pKa = 4.48 EE557 pKa = 4.04 VRR559 pKa = 11.84 GRR561 pKa = 11.84 GQVCRR566 pKa = 11.84 VEE568 pKa = 4.58 LDD570 pKa = 3.35 SFRR573 pKa = 11.84 AGQGFGNLIVKK584 pKa = 8.19 MSRR587 pKa = 11.84 RR588 pKa = 11.84 SSDD591 pKa = 2.41 IATSRR596 pKa = 11.84 EE597 pKa = 3.87 VNPWLRR603 pKa = 11.84 LQSGPQTLPDD613 pKa = 4.08 RR614 pKa = 11.84 VTDD617 pKa = 3.61 DD618 pKa = 4.08 HH619 pKa = 6.83 PVCFTRR625 pKa = 11.84 SDD627 pKa = 3.6 NAGLCSQLRR636 pKa = 11.84 NHH638 pKa = 7.45 RR639 pKa = 11.84 GPHH642 pKa = 5.68 IGATACADD650 pKa = 3.14 IRR652 pKa = 11.84 ADD654 pKa = 3.74 ALDD657 pKa = 3.74 GGLRR661 pKa = 11.84 YY662 pKa = 10.04 DD663 pKa = 4.21 FKK665 pKa = 11.14 IILLRR670 pKa = 11.84 VNAGVVSFFDD680 pKa = 3.61 PKK682 pKa = 10.95 VDD684 pKa = 3.56 VVDD687 pKa = 4.06 RR688 pKa = 11.84 QGLSDD693 pKa = 3.48 RR694 pKa = 11.84 FKK696 pKa = 11.17 VRR698 pKa = 11.84 RR699 pKa = 11.84 CKK701 pKa = 10.4 QPEE704 pKa = 4.02 FLALTTPGPAHH715 pKa = 7.04 RR716 pKa = 11.84 FGSRR720 pKa = 11.84 GRR722 pKa = 11.84 EE723 pKa = 4.22 VNCHH727 pKa = 7.04 LIAEE731 pKa = 4.71 RR732 pKa = 11.84 CSLTCPPCSRR742 pKa = 11.84 PVIEE746 pKa = 4.83 RR747 pKa = 11.84 VEE749 pKa = 4.02 EE750 pKa = 3.91 FHH752 pKa = 7.38 WNFLVGSGGEE762 pKa = 4.27 FIAVLDD768 pKa = 3.87 EE769 pKa = 4.34 CAPLLLMCRR778 pKa = 11.84 QRR780 pKa = 11.84 HH781 pKa = 5.59 RR782 pKa = 11.84 SPQSGNLSEE791 pKa = 5.46 GRR793 pKa = 11.84 GALNRR798 pKa = 11.84 TPKK801 pKa = 10.45 EE802 pKa = 3.98 SLKK805 pKa = 10.71 RR806 pKa = 11.84 PSLEE810 pKa = 3.8 CVEE813 pKa = 4.86 RR814 pKa = 11.84 EE815 pKa = 4.06 TPRR818 pKa = 11.84 RR819 pKa = 11.84 TRR821 pKa = 11.84 LGLPGVDD828 pKa = 3.07 RR829 pKa = 11.84 ARR831 pKa = 11.84 ATVLCVLFNTLDD843 pKa = 3.85 GFFSAHH849 pKa = 6.9 KK850 pKa = 10.52 GPGLFQPFAEE860 pKa = 4.56 LQEE863 pKa = 4.61 SLCSSPMARR872 pKa = 11.84 IRR874 pKa = 11.84 QFPSAPLQGWEE885 pKa = 4.12 KK886 pKa = 10.77 RR887 pKa = 11.84 EE888 pKa = 3.7 YY889 pKa = 10.09 DD890 pKa = 3.81 QPEE893 pKa = 4.37 TVPNTVDD900 pKa = 3.21 CVEE903 pKa = 4.49 IFDD906 pKa = 5.21 AGWAQICRR914 pKa = 11.84 LLGAFRR920 pKa = 11.84 GQSRR924 pKa = 11.84 GGGRR928 pKa = 11.84 TPLLLVGPLRR938 pKa = 11.84 GDD940 pKa = 3.24 QNRR943 pKa = 11.84 PVSKK947 pKa = 10.8 SRR949 pKa = 11.84 WW950 pKa = 3.45
Molecular weight: 106.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.341
IPC_protein 10.16
Toseland 10.613
ProMoST 10.394
Dawson 10.687
Bjellqvist 10.467
Wikipedia 10.921
Rodwell 10.701
Grimsley 10.716
Solomon 10.862
Lehninger 10.833
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.613
EMBOSS 11.038
Sillero 10.643
Patrickios 10.394
IPC_peptide 10.877
IPC2_peptide 9.94
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1796
846
950
898.0
99.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.134 ± 2.463
2.394 ± 0.753
4.677 ± 0.523
5.902 ± 0.412
3.452 ± 0.342
7.962 ± 0.459
3.062 ± 0.481
3.842 ± 0.203
2.951 ± 0.247
8.964 ± 0.176
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.615 ± 0.19
2.561 ± 0.787
6.849 ± 0.809
3.563 ± 0.826
12.194 ± 0.664
6.347 ± 0.87
4.51 ± 0.013
6.514 ± 0.154
1.002 ± 0.205
1.503 ± 0.511
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here