Changjiang polero-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KED2|A0A1L3KED2_9VIRU RNA-directed RNA polymerase OS=Changjiang polero-like virus 1 OX=1922799 PE=4 SV=1
MM1 pKa = 7.26NSSYY5 pKa = 10.62IDD7 pKa = 3.92SRR9 pKa = 11.84PLSYY13 pKa = 11.64VNMYY17 pKa = 9.74KK18 pKa = 9.63WEE20 pKa = 4.19DD21 pKa = 3.86EE22 pKa = 4.17KK23 pKa = 11.08WSTVHH28 pKa = 6.24LTAGYY33 pKa = 9.78SRR35 pKa = 11.84NDD37 pKa = 3.51GEE39 pKa = 4.8EE40 pKa = 3.67IQPYY44 pKa = 8.87MVIPASKK51 pKa = 10.44GKK53 pKa = 9.76FRR55 pKa = 11.84VYY57 pKa = 10.42LEE59 pKa = 4.72CEE61 pKa = 3.53GFMAVKK67 pKa = 10.45SIGGKK72 pKa = 10.0ADD74 pKa = 3.55GCWAGLMAYY83 pKa = 10.17DD84 pKa = 4.05STKK87 pKa = 10.77SGWSVSEE94 pKa = 3.82YY95 pKa = 10.91VGAKK99 pKa = 7.34ITNIQANTNFVCGHH113 pKa = 7.31PDD115 pKa = 3.19VKK117 pKa = 11.3LNDD120 pKa = 3.5CTFKK124 pKa = 10.8KK125 pKa = 10.53NRR127 pKa = 11.84GIEE130 pKa = 4.03CDD132 pKa = 4.16FVCSFHH138 pKa = 7.53LDD140 pKa = 3.49CDD142 pKa = 4.23DD143 pKa = 5.97EE144 pKa = 5.57DD145 pKa = 4.79GKK147 pKa = 10.28WLLYY151 pKa = 9.2PPPVQKK157 pKa = 10.79SDD159 pKa = 3.39DD160 pKa = 3.54FNYY163 pKa = 9.85IVSYY167 pKa = 11.69SNFTDD172 pKa = 3.94KK173 pKa = 10.45WLEE176 pKa = 3.55WGMISISIDD185 pKa = 3.64EE186 pKa = 4.49INSPSSLQGLPRR198 pKa = 11.84RR199 pKa = 11.84RR200 pKa = 11.84LTQQLTTPEE209 pKa = 4.51GQPDD213 pKa = 3.92TNPAIGEE220 pKa = 4.27QATGPAPILSPNPALGEE237 pKa = 4.09EE238 pKa = 4.29KK239 pKa = 10.76LEE241 pKa = 4.73SIQRR245 pKa = 11.84WLSFSPSDD253 pKa = 3.93SEE255 pKa = 4.3QDD257 pKa = 2.97IPAPPDD263 pKa = 3.4PEE265 pKa = 5.37RR266 pKa = 11.84PTLPQEE272 pKa = 4.45VFRR275 pKa = 11.84VPPSNSIPSVIAPSLAGGSLRR296 pKa = 11.84GSLFGGSLKK305 pKa = 10.3PRR307 pKa = 11.84SLDD310 pKa = 3.39SGKK313 pKa = 10.58LPEE316 pKa = 4.21TTPRR320 pKa = 11.84DD321 pKa = 3.82NVSVAGSLTGSLVGGSLRR339 pKa = 11.84GGALKK344 pKa = 10.54SPRR347 pKa = 11.84LDD349 pKa = 2.97TAQAKK354 pKa = 8.46EE355 pKa = 3.75HH356 pKa = 6.35ARR358 pKa = 11.84ILKK361 pKa = 9.93QLGKK365 pKa = 8.1TAAAEE370 pKa = 3.8YY371 pKa = 10.15LEE373 pKa = 4.43RR374 pKa = 11.84VRR376 pKa = 11.84ASQSSGQDD384 pKa = 3.09FKK386 pKa = 11.88

Molecular weight: 42.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEC6|A0A1L3KEC6_9VIRU Uncharacterized protein OS=Changjiang polero-like virus 1 OX=1922799 PE=4 SV=1
MM1 pKa = 7.34VIAISWMFGGLPGFLFGVALWLLKK25 pKa = 10.77SGWRR29 pKa = 11.84VLTSGPSYY37 pKa = 10.1MKK39 pKa = 9.8EE40 pKa = 3.84KK41 pKa = 10.69AVAGYY46 pKa = 10.38LSYY49 pKa = 10.4TIPQEE54 pKa = 4.03PPKK57 pKa = 10.57KK58 pKa = 10.5SIVLIQNSDD67 pKa = 3.35GSHH70 pKa = 6.46AGYY73 pKa = 7.72GTCVRR78 pKa = 11.84LYY80 pKa = 10.64NGEE83 pKa = 4.26TALLTADD90 pKa = 3.91HH91 pKa = 6.77VYY93 pKa = 10.96DD94 pKa = 3.93PALLVVSARR103 pKa = 11.84NGTKK107 pKa = 10.07VPLSQFQPVVRR118 pKa = 11.84GKK120 pKa = 8.78EE121 pKa = 3.57TDD123 pKa = 4.22LIILRR128 pKa = 11.84GPPNWEE134 pKa = 3.49GLLATKK140 pKa = 10.14AVQTVPVSQLAKK152 pKa = 10.45SKK154 pKa = 10.51FSLYY158 pKa = 10.83SFDD161 pKa = 4.95GAWQATNGEE170 pKa = 4.35LVGVEE175 pKa = 4.12GGFATTLCNTTPGFSGTPLFSGKK198 pKa = 9.73RR199 pKa = 11.84VVGVHH204 pKa = 5.67VGASKK209 pKa = 10.47KK210 pKa = 11.03ANVNLMAPIPSIAGLTSPQYY230 pKa = 10.93VFEE233 pKa = 4.4TTAPTGRR240 pKa = 11.84LFTSDD245 pKa = 3.25EE246 pKa = 4.06AVQLSEE252 pKa = 4.26TFQVLHH258 pKa = 6.61SEE260 pKa = 4.29LEE262 pKa = 3.97KK263 pKa = 11.17VLNFKK268 pKa = 10.29SQSGKK273 pKa = 8.72QWSEE277 pKa = 3.58MLDD280 pKa = 3.19IEE282 pKa = 4.66TTPTAPTPAVKK293 pKa = 10.47APVPAIQGNEE303 pKa = 3.55PRR305 pKa = 11.84GADD308 pKa = 2.87RR309 pKa = 11.84VINGEE314 pKa = 3.91ATVDD318 pKa = 3.49SGSTVATRR326 pKa = 11.84RR327 pKa = 11.84TSPVDD332 pKa = 3.43GEE334 pKa = 5.12DD335 pKa = 3.24ILAKK339 pKa = 10.75LMASLMSKK347 pKa = 10.39VDD349 pKa = 3.3VKK351 pKa = 11.08SIEE354 pKa = 3.99KK355 pKa = 9.88EE356 pKa = 3.95AIRR359 pKa = 11.84MISEE363 pKa = 4.32KK364 pKa = 10.62ALKK367 pKa = 9.88APRR370 pKa = 11.84RR371 pKa = 11.84RR372 pKa = 11.84GPRR375 pKa = 11.84KK376 pKa = 9.16PRR378 pKa = 11.84TSRR381 pKa = 11.84PSSIPTTPGKK391 pKa = 10.16YY392 pKa = 9.31VPPQRR397 pKa = 11.84RR398 pKa = 11.84VTHH401 pKa = 5.32QQASSGPANSPASTTLNKK419 pKa = 9.68EE420 pKa = 4.19AKK422 pKa = 9.73QSGGKK427 pKa = 9.43HH428 pKa = 5.06SPGTIQRR435 pKa = 11.84WVRR438 pKa = 11.84KK439 pKa = 9.54SEE441 pKa = 3.85ASAGRR446 pKa = 11.84CSAPKK451 pKa = 10.24QSS453 pKa = 3.47

Molecular weight: 48.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1220

381

453

406.7

44.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.295 ± 0.686

1.393 ± 0.325

4.754 ± 0.94

5.656 ± 0.363

3.279 ± 0.255

7.787 ± 0.758

1.967 ± 0.636

3.934 ± 0.438

5.246 ± 0.824

9.344 ± 1.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.885 ± 0.182

3.607 ± 0.335

7.049 ± 0.768

4.18 ± 0.124

5.164 ± 0.242

10.082 ± 0.535

5.656 ± 1.031

7.295 ± 0.889

1.967 ± 0.198

2.459 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski