Abyssibacter profundi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Salinisphaerales; Salinisphaeraceae; Abyssibacter

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3331 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A383XPS3|A0A383XPS3_9GAMM Uncharacterized protein OS=Abyssibacter profundi OX=2182787 GN=DEH80_16535 PE=4 SV=1
MM1 pKa = 7.3FEE3 pKa = 4.18SARR6 pKa = 11.84SRR8 pKa = 11.84VLIAGIASTCSLSALAQQSGPTACDD33 pKa = 2.84IAFGAVEE40 pKa = 4.3SGAVAAAIDD49 pKa = 4.01PALQPGKK56 pKa = 9.08FQRR59 pKa = 11.84IVLTFADD66 pKa = 3.82AAARR70 pKa = 11.84DD71 pKa = 3.66AALEE75 pKa = 4.0RR76 pKa = 11.84VRR78 pKa = 11.84LANFLPVDD86 pKa = 4.03VARR89 pKa = 11.84DD90 pKa = 3.83AYY92 pKa = 10.78AMRR95 pKa = 11.84HH96 pKa = 5.25LNFVVLPALTVSTDD110 pKa = 4.29LLDD113 pKa = 3.48QLAALGRR120 pKa = 11.84DD121 pKa = 3.26LGLISVSTDD130 pKa = 3.05PEE132 pKa = 4.14VRR134 pKa = 11.84SLIDD138 pKa = 3.04STGPLIGVNAARR150 pKa = 11.84AAFGEE155 pKa = 4.46GKK157 pKa = 10.06EE158 pKa = 4.02PAGYY162 pKa = 7.95TVTGDD167 pKa = 3.9GIGVAVLDD175 pKa = 4.13TGWDD179 pKa = 3.24KK180 pKa = 10.16TQGDD184 pKa = 4.51FEE186 pKa = 4.5HH187 pKa = 7.19LEE189 pKa = 4.1TFGNVRR195 pKa = 11.84MVGPQAIEE203 pKa = 3.63FVPPVEE209 pKa = 4.5NSEE212 pKa = 4.31TNQGHH217 pKa = 5.43GTHH220 pKa = 6.31IVGTIAGNGAQSDD233 pKa = 3.86GRR235 pKa = 11.84IVGLAPQVNMLSVAVDD251 pKa = 3.74AGASYY256 pKa = 11.58AFLLDD261 pKa = 3.4GFDD264 pKa = 3.97YY265 pKa = 11.07VLEE268 pKa = 4.32VQDD271 pKa = 4.82DD272 pKa = 3.98YY273 pKa = 11.88NIRR276 pKa = 11.84VTNHH280 pKa = 6.04SYY282 pKa = 11.19GPAVGSAFRR291 pKa = 11.84FDD293 pKa = 4.1PSTASSQAIRR303 pKa = 11.84AFYY306 pKa = 9.37QAGIIPVFAAGNAGPDD322 pKa = 3.71EE323 pKa = 4.4DD324 pKa = 5.29TISADD329 pKa = 3.39AQNPCAIGVAAGTRR343 pKa = 11.84ALTLTDD349 pKa = 4.42FSSRR353 pKa = 11.84GRR355 pKa = 11.84SDD357 pKa = 3.21NAIAGPDD364 pKa = 2.94ITAPGDD370 pKa = 3.91AITASRR376 pKa = 11.84AINGFTSTSIPDD388 pKa = 4.03LDD390 pKa = 3.83NPAYY394 pKa = 9.53ATISGTSMAAPHH406 pKa = 5.74VVASIALILEE416 pKa = 4.41ADD418 pKa = 3.63EE419 pKa = 4.58SLSFEE424 pKa = 4.6DD425 pKa = 4.14VYY427 pKa = 11.71EE428 pKa = 5.29LITSTATPMKK438 pKa = 10.37RR439 pKa = 11.84ADD441 pKa = 3.36QTAYY445 pKa = 10.26EE446 pKa = 4.09PFEE449 pKa = 4.25VGFGYY454 pKa = 10.1IDD456 pKa = 3.36TAAAIAKK463 pKa = 9.87LLGEE467 pKa = 4.29QKK469 pKa = 10.33PEE471 pKa = 3.52RR472 pKa = 11.84LQPVEE477 pKa = 4.47CPAPGEE483 pKa = 4.06SSVINFAATAAAVYY497 pKa = 7.41STPAVSFSPSGFEE510 pKa = 3.78DD511 pKa = 3.62SIYY514 pKa = 10.91QFLLEE519 pKa = 4.63PSCALASMTVEE530 pKa = 4.41IEE532 pKa = 3.81WSATLPEE539 pKa = 5.75DD540 pKa = 4.85IDD542 pKa = 4.37LEE544 pKa = 4.71LIGPEE549 pKa = 4.09GGVIGASTNRR559 pKa = 11.84QLEE562 pKa = 4.24EE563 pKa = 3.76GAEE566 pKa = 3.98PSEE569 pKa = 4.0AVTIEE574 pKa = 4.11SAGGGLYY581 pKa = 9.53TSRR584 pKa = 11.84VFGYY588 pKa = 10.51RR589 pKa = 11.84NNNLPYY595 pKa = 9.02TGTVTLVAEE604 pKa = 4.42QNINTAPVAALSVPATAGVDD624 pKa = 3.54EE625 pKa = 5.71EE626 pKa = 4.67ITVSAAASSDD636 pKa = 3.59PDD638 pKa = 3.72GDD640 pKa = 3.81ALNYY644 pKa = 10.37QFDD647 pKa = 4.57LGDD650 pKa = 3.78GTQTTVSTSPTVTHH664 pKa = 7.34RR665 pKa = 11.84YY666 pKa = 7.28ATAGDD671 pKa = 3.88YY672 pKa = 10.93VVTVTVSDD680 pKa = 3.55ASGATDD686 pKa = 3.73SASQTITVAEE696 pKa = 4.47DD697 pKa = 3.35GGGNTAGPGSTIDD710 pKa = 3.32AVLAVSGDD718 pKa = 3.62GAEE721 pKa = 4.24SDD723 pKa = 3.63GDD725 pKa = 4.1AVNGDD730 pKa = 3.75AEE732 pKa = 4.76TVFTFDD738 pKa = 5.08ASDD741 pKa = 3.08SGYY744 pKa = 9.34TDD746 pKa = 4.58AEE748 pKa = 4.41GSDD751 pKa = 3.72LYY753 pKa = 10.52YY754 pKa = 10.78TFVFGDD760 pKa = 3.56EE761 pKa = 4.21ASEE764 pKa = 4.2EE765 pKa = 4.69EE766 pKa = 4.59YY767 pKa = 11.12ASTTSDD773 pKa = 4.15PIATHH778 pKa = 6.63SYY780 pKa = 9.35GAAGTYY786 pKa = 7.26EE787 pKa = 4.3AYY789 pKa = 10.8VIVSDD794 pKa = 4.82AFGNSDD800 pKa = 3.39TSNTVTITTTITITVEE816 pKa = 3.83GDD818 pKa = 2.93NGTVAQLTVDD828 pKa = 4.03EE829 pKa = 4.76TSGTAPLQVVFDD841 pKa = 4.49GSQSFTSEE849 pKa = 4.07GEE851 pKa = 4.36TITEE855 pKa = 4.07YY856 pKa = 11.19CFDD859 pKa = 4.58FDD861 pKa = 5.92DD862 pKa = 6.4GEE864 pKa = 4.53APQCGTEE871 pKa = 4.01DD872 pKa = 3.45TATYY876 pKa = 10.56VYY878 pKa = 9.26TRR880 pKa = 11.84PGSYY884 pKa = 9.97EE885 pKa = 3.58PSLTVTASDD894 pKa = 4.06EE895 pKa = 4.31STATAKK901 pKa = 9.97ATVSVGGTGSNPGTPSQPAASTGGGSGALGGLLLLPLL938 pKa = 4.51

Molecular weight: 96.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A383XR84|A0A383XR84_9GAMM TetR/AcrR family transcriptional regulator OS=Abyssibacter profundi OX=2182787 GN=DEH80_14020 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 9.53QTFQPHH8 pKa = 5.02AVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.53RR14 pKa = 11.84THH16 pKa = 5.8GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.18VIKK32 pKa = 10.13ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.81GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LTPP44 pKa = 3.95

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3331

0

3331

1138768

33

4044

341.9

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.074 ± 0.058

0.948 ± 0.014

6.336 ± 0.046

5.723 ± 0.039

3.308 ± 0.024

8.296 ± 0.047

2.184 ± 0.021

4.432 ± 0.03

2.252 ± 0.033

10.579 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.018

2.509 ± 0.023

5.263 ± 0.036

4.214 ± 0.03

7.429 ± 0.045

5.528 ± 0.033

5.425 ± 0.04

7.503 ± 0.034

1.458 ± 0.019

2.324 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski