Meiothermus luteus
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2850 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A399F4D6|A0A399F4D6_9DEIN Uncharacterized protein OS=Meiothermus luteus OX=2026184 GN=Mlute_00041 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 EE3 pKa = 4.09 EE4 pKa = 4.95 LCPEE8 pKa = 4.15 CQGDD12 pKa = 4.47 GYY14 pKa = 10.02 VTVACDD20 pKa = 3.93 YY21 pKa = 10.53 YY22 pKa = 10.16 SASYY26 pKa = 10.36 GYY28 pKa = 10.05 YY29 pKa = 10.2 LQDD32 pKa = 3.65 EE33 pKa = 4.72 RR34 pKa = 11.84 PVPCRR39 pKa = 11.84 MCGGSGYY46 pKa = 10.04 VEE48 pKa = 4.03 VMEE51 pKa = 5.34 AEE53 pKa = 4.48 VAEE56 pKa = 4.48 EE57 pKa = 3.86 AAA59 pKa = 4.45
Molecular weight: 6.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.961
IPC2_protein 3.986
IPC_protein 3.795
Toseland 3.643
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.643
Grimsley 3.579
Solomon 3.719
Lehninger 3.668
Nozaki 3.897
DTASelect 3.948
Thurlkill 3.694
EMBOSS 3.656
Sillero 3.91
Patrickios 0.006
IPC_peptide 3.719
IPC2_peptide 3.884
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A399EAS6|A0A399EAS6_9DEIN 30S ribosomal protein S7 OS=Meiothermus luteus OX=2026184 GN=rpsG PE=3 SV=1
MM1 pKa = 7.22 IRR3 pKa = 11.84 VSGTSSMRR11 pKa = 11.84 ALKK14 pKa = 5.88 TTPWARR20 pKa = 11.84 ARR22 pKa = 11.84 PMTTHH27 pKa = 7.93 PITNQRR33 pKa = 11.84 FIPNHH38 pKa = 6.06 LLHH41 pKa = 6.29 FTAPKK46 pKa = 9.9 VRR48 pKa = 11.84 PATRR52 pKa = 3.24
Molecular weight: 5.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2850
0
2850
849109
29
1852
297.9
32.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.059 ± 0.049
0.575 ± 0.012
3.695 ± 0.028
7.364 ± 0.051
3.596 ± 0.034
8.953 ± 0.04
1.937 ± 0.023
3.59 ± 0.034
3.054 ± 0.033
13.146 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.792 ± 0.02
2.183 ± 0.027
6.075 ± 0.036
3.865 ± 0.027
7.988 ± 0.051
4.53 ± 0.031
4.292 ± 0.033
7.806 ± 0.043
1.614 ± 0.019
2.884 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here