Faecalitalea cylindroides T2-87
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1442 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4JG29|D4JG29_9FIRM ABC-2 type transporter OS=Faecalitalea cylindroides T2-87 OX=717960 GN=EC1_19200 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.37 KK3 pKa = 9.86 IFTFFISTFMILNLAACSSNNDD25 pKa = 3.26 DD26 pKa = 3.67 YY27 pKa = 12.08 ALEE30 pKa = 4.35 FFSALDD36 pKa = 3.37 NTLEE40 pKa = 4.34 LNSGHH45 pKa = 6.16 IQGTFTSNNEE55 pKa = 3.89 DD56 pKa = 3.07 ASKK59 pKa = 10.76 IKK61 pKa = 10.52 FDD63 pKa = 3.9 LQLNQKK69 pKa = 10.75 DD70 pKa = 4.07 NLQLALDD77 pKa = 4.74 LDD79 pKa = 4.52 LEE81 pKa = 5.24 AGDD84 pKa = 3.84 NAEE87 pKa = 4.94 DD88 pKa = 3.67 NFLNFYY94 pKa = 10.68 IKK96 pKa = 10.5 DD97 pKa = 3.32 NKK99 pKa = 10.29 TYY101 pKa = 10.45 LNSYY105 pKa = 7.77 GTTSQSLLEE114 pKa = 3.93 NLGINGSEE122 pKa = 4.17 KK123 pKa = 10.65 LSVYY127 pKa = 10.82 NPFLNYY133 pKa = 9.91 TDD135 pKa = 5.72 DD136 pKa = 3.62 EE137 pKa = 4.59 LKK139 pKa = 11.1 ALFTRR144 pKa = 11.84 SSKK147 pKa = 10.93 NGNNYY152 pKa = 10.1 SFTIDD157 pKa = 3.18 GSLISSYY164 pKa = 10.27 LDD166 pKa = 3.01 SMGSVTIEE174 pKa = 4.64 DD175 pKa = 3.67 ATLDD179 pKa = 3.42 ATIEE183 pKa = 4.11 DD184 pKa = 4.26 NYY186 pKa = 9.6 ITSLTLGISGLQDD199 pKa = 3.09 VEE201 pKa = 4.41 TQQVIIDD208 pKa = 3.7 VTIEE212 pKa = 4.06 CTLDD216 pKa = 3.3 QINSLDD222 pKa = 3.82 TVTYY226 pKa = 9.37 PADD229 pKa = 3.68 LEE231 pKa = 4.53 NYY233 pKa = 9.49
Molecular weight: 25.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.516
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.859
Patrickios 1.1
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|D4JEF5|D4JEF5_9FIRM Uncharacterized protein OS=Faecalitalea cylindroides T2-87 OX=717960 GN=EC1_10460 PE=4 SV=1
MM1 pKa = 8.04 DD2 pKa = 4.82 KK3 pKa = 10.86 LFRR6 pKa = 11.84 SDD8 pKa = 5.24 QRR10 pKa = 11.84 PCDD13 pKa = 3.83 PDD15 pKa = 4.8 GGTSGAMRR23 pKa = 11.84 ARR25 pKa = 11.84 GLKK28 pKa = 9.03 DD29 pKa = 3.16 TSSRR33 pKa = 11.84 PAKK36 pKa = 10.44 DD37 pKa = 2.94 LGGTLRR43 pKa = 11.84 HH44 pKa = 6.6 TIRR47 pKa = 11.84 TMLRR51 pKa = 11.84 SYY53 pKa = 11.13 DD54 pKa = 3.46 SRR56 pKa = 11.84 ASGDD60 pKa = 3.78 LLCSHH65 pKa = 6.88 RR66 pKa = 11.84 QGGVANMASNPNRR79 pKa = 11.84 ITVAAPGFYY88 pKa = 10.09 PKK90 pKa = 10.1 ISFRR94 pKa = 11.84 LVRR97 pKa = 11.84 LFKK100 pKa = 10.96 YY101 pKa = 10.43
Molecular weight: 11.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.736
IPC_protein 10.701
Toseland 10.789
ProMoST 10.613
Dawson 10.877
Bjellqvist 10.643
Wikipedia 11.125
Rodwell 10.994
Grimsley 10.921
Solomon 11.052
Lehninger 11.008
Nozaki 10.789
DTASelect 10.628
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.818
Patrickios 10.76
IPC_peptide 11.052
IPC2_peptide 9.809
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1442
0
1442
362689
15
3253
251.5
28.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.608 ± 0.081
1.505 ± 0.029
6.057 ± 0.055
7.344 ± 0.083
4.197 ± 0.052
6.128 ± 0.058
1.935 ± 0.029
8.01 ± 0.085
7.554 ± 0.081
9.163 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.15 ± 0.043
4.755 ± 0.053
3.147 ± 0.037
3.706 ± 0.05
3.952 ± 0.072
5.948 ± 0.049
5.259 ± 0.062
6.6 ± 0.063
0.822 ± 0.022
4.161 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here