Rotavirus A turkey-tc/GER/03V0002E10/2003/G22P[35]

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus A isolates

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0CQJ0|L0CQJ0_9REOV Non-structural protein 5 OS=Rotavirus A turkey-tc/GER/03V0002E10/2003/G22P[35] OX=1271535 PE=4 SV=1
MM1 pKa = 7.48YY2 pKa = 9.47STEE5 pKa = 4.05CTILSIEE12 pKa = 4.27IIFYY16 pKa = 10.62CLAAILLYY24 pKa = 11.28DD25 pKa = 3.93MLCRR29 pKa = 11.84MANSPLFCFSVLIGVVTILPRR50 pKa = 11.84CYY52 pKa = 10.14AQNYY56 pKa = 7.88GINVPITGSLDD67 pKa = 3.25VTVQNQTSEE76 pKa = 4.72PIGLTSTLCIYY87 pKa = 10.87YY88 pKa = 8.33PTEE91 pKa = 4.02AANEE95 pKa = 4.11IADD98 pKa = 4.27SEE100 pKa = 4.43WKK102 pKa = 8.15QTVTQLFLTKK112 pKa = 9.98GWPTTSIYY120 pKa = 10.86LNEE123 pKa = 4.18YY124 pKa = 9.69TDD126 pKa = 4.62LQTFSNNPQLNCDD139 pKa = 3.73YY140 pKa = 11.12NIVLVKK146 pKa = 10.63YY147 pKa = 10.35DD148 pKa = 3.86GNQGLDD154 pKa = 2.95ISEE157 pKa = 4.24LAEE160 pKa = 3.99LLLYY164 pKa = 10.19EE165 pKa = 4.32WLCNEE170 pKa = 4.36MDD172 pKa = 3.29VSLYY176 pKa = 10.67YY177 pKa = 9.88YY178 pKa = 10.06QQVSEE183 pKa = 4.16ANKK186 pKa = 8.42WIAMGTDD193 pKa = 3.77CTIKK197 pKa = 10.42VCPLNTQTLGIGCKK211 pKa = 7.88TTDD214 pKa = 3.2LTTFEE219 pKa = 4.48QLTASEE225 pKa = 4.47KK226 pKa = 10.41LAIVDD231 pKa = 3.63VVDD234 pKa = 4.5GVNHH238 pKa = 6.81KK239 pKa = 9.93IDD241 pKa = 3.84YY242 pKa = 8.11TVTTCNVKK250 pKa = 10.05NCLRR254 pKa = 11.84LSQRR258 pKa = 11.84EE259 pKa = 4.01NVAIIQVGGPEE270 pKa = 4.31VIDD273 pKa = 3.73VSEE276 pKa = 5.04DD277 pKa = 3.34PMVVPKK283 pKa = 8.42MQRR286 pKa = 11.84VTRR289 pKa = 11.84INWKK293 pKa = 9.4KK294 pKa = 7.32WWQVFYY300 pKa = 10.64TIVDD304 pKa = 3.85YY305 pKa = 11.16INTIIQTMSKK315 pKa = 10.31RR316 pKa = 11.84SRR318 pKa = 11.84SLNASAYY325 pKa = 8.0YY326 pKa = 10.36FRR328 pKa = 11.84VV329 pKa = 3.31

Molecular weight: 37.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0CS97|L0CS97_9REOV Intermediate capsid protein VP6 OS=Rotavirus A turkey-tc/GER/03V0002E10/2003/G22P[35] OX=1271535 PE=3 SV=1
MM1 pKa = 6.85NHH3 pKa = 5.71LVKK6 pKa = 10.64RR7 pKa = 11.84QVLQEE12 pKa = 3.9NLLVGVSSMYY22 pKa = 10.39HH23 pKa = 5.96CPKK26 pKa = 9.77KK27 pKa = 10.27HH28 pKa = 6.21LVNTCLIGIQKK39 pKa = 8.46TLDD42 pKa = 3.36HH43 pKa = 7.23LIQHH47 pKa = 6.18QMTCPPNLLLNQMQLNQMLMLVCRR71 pKa = 11.84WMLVQQIVRR80 pKa = 11.84QEE82 pKa = 3.77AMQKK86 pKa = 8.72MKK88 pKa = 10.82SIFHH92 pKa = 5.94SLKK95 pKa = 10.27EE96 pKa = 3.95

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5987

96

1089

498.9

57.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.428 ± 0.318

1.353 ± 0.424

6.314 ± 0.274

5.345 ± 0.238

4.092 ± 0.232

3.341 ± 0.391

1.57 ± 0.278

7.717 ± 0.221

6.731 ± 0.557

9.237 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.247

6.798 ± 0.339

3.207 ± 0.294

4.343 ± 0.491

4.76 ± 0.315

7.65 ± 0.737

6.347 ± 0.415

6.648 ± 0.346

1.086 ± 0.165

5.295 ± 0.688
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski