Mycolicibacterium insubricum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X0CTG4|A0A1X0CTG4_9MYCO Pseudouridine synthase OS=Mycolicibacterium insubricum OX=444597 GN=BST26_20480 PE=3 SV=1
MM1 pKa = 7.76ARR3 pKa = 11.84RR4 pKa = 11.84TPEE7 pKa = 3.95TVVGVDD13 pKa = 3.86WPPVIPPLWDD23 pKa = 3.68PAPEE27 pKa = 4.51CGYY30 pKa = 11.28DD31 pKa = 3.74DD32 pKa = 5.61AADD35 pKa = 4.03LEE37 pKa = 4.77DD38 pKa = 4.58WVPLPPDD45 pKa = 4.11HH46 pKa = 6.98EE47 pKa = 4.59WPALDD52 pKa = 4.91PDD54 pKa = 5.0LEE56 pKa = 5.3ALLLGRR62 pKa = 11.84GWVLATHH69 pKa = 7.29AGDD72 pKa = 3.91CDD74 pKa = 4.8FYY76 pKa = 11.47DD77 pKa = 4.0WPASEE82 pKa = 5.53HH83 pKa = 7.08DD84 pKa = 4.06FGDD87 pKa = 3.77TEE89 pKa = 4.67LEE91 pKa = 4.04ISAMTYY97 pKa = 8.6IGVMIPEE104 pKa = 4.39SEE106 pKa = 4.22SEE108 pKa = 3.93EE109 pKa = 3.87LHH111 pKa = 6.4YY112 pKa = 10.83DD113 pKa = 3.4VHH115 pKa = 8.01HH116 pKa = 6.71VGPEE120 pKa = 3.92VGSSRR125 pKa = 11.84SYY127 pKa = 10.09TDD129 pKa = 3.1RR130 pKa = 11.84AALIADD136 pKa = 4.54LDD138 pKa = 4.6DD139 pKa = 4.54IEE141 pKa = 4.33AVRR144 pKa = 11.84YY145 pKa = 9.83VPGGQQ150 pKa = 3.06

Molecular weight: 16.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X0DHE0|A0A1X0DHE0_9MYCO NUDIX hydrolase OS=Mycolicibacterium insubricum OX=444597 GN=BST26_07010 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSGRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.81GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4200

0

4200

1350420

27

3072

321.5

34.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.499 ± 0.053

0.796 ± 0.01

6.386 ± 0.032

5.042 ± 0.037

2.954 ± 0.017

9.16 ± 0.038

2.107 ± 0.016

4.248 ± 0.022

2.06 ± 0.025

9.875 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.017

2.294 ± 0.024

5.983 ± 0.033

2.815 ± 0.022

7.149 ± 0.038

5.174 ± 0.024

6.257 ± 0.036

8.557 ± 0.034

1.473 ± 0.014

2.149 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski